Vial | Cap | Label | Function | Content |
---|---|---|---|---|
1 | red | LightCycler® 480 RNA Master Hydrolysis Probes, 2.7x conc. | Contains Tth DNA Polymerase, reaction buffer and dNTP mix (with dUTP instead of dTTP). | 5 vials, 740 μl each |
2 | colorless, grey cap label | Activator, 50 mM | To adjust Mn (OAc)2 concentration. | 1 ml |
3 | colorless | Water, PCR grade | To adjust the final reaction volume. | 5 vials, 1 ml each |
4 | yellow | Enhancer, 20x conc. | To improve the amplification of difficult (e.g., GC-rich) targets. | 500 μl |
Easy-to-use Reaction Mix for One-Step RT-PCR using the LightCycler® 480 or LightCycler® 96 Real-Time PCR Systems.