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Note: Sealing foils are also included in the LightCycler^® 480 Multiwell Plate 96, white, LightCycler^® 480 Multiwell Plate 96, clear, LightCycler^® 480 Multiwell Plate 384, white, and LightCycler^® 480 Multiwell Plate 384, clear packs.


Complete Sealing

Make sure to position the sealing foil covering the entire plate and to seal properly. The MWP (multiwell plates) need to be centrifuged (3000 rpm for 2 min) prior to the run: Roche recommends visually checking for any air bubbles or drops in the wells containing reagents (if these are found always repeat the centrifugation step).


Alternative Seal for the MTPs

Since the geometry of the MTPs is compatible with the standard dimensions of multiwell plates, any standard seal, e.g., plastic PCR caps, may in principle be used to cover the plates. However, when the MTPs are inserted in the LightCycler^® 480 Instrument, the adhesive LightCycler^® 480 Sealing Foils must be used for the following reasons:
- Transparency of the LightCycler^® 480 sealing foils is tested to give best results regarding detection of fluorescence signals.
- The foil is pressed onto the plate with a force of more than 500 N, thus producing a tight seal. The use of a different cover may lead to a leak as it may not fit the plate perfectly.

Note: Before loading the PCR multiwell plate into the LightCycler^® 480 Instrument, it has to be sealed properly with the self-adhesive sealing foil. Sealing the plate is crucial to eliminate evaporation at high temperatures. Use only the recommended foil. Always centrifuge the filled and sealed plate before loading it into the instrument. The foil should be applied at the end of the PCR setup, as repeated opening and closing will lead to cross-contamination.", "Country": "XG", "Code": "Background Information - Help Corner", "Name": "Background Information - Help Corner" }, { "Language": "en", "Value": "Each LightCycler^® 480 Sealing Foil Pack contains 50 foils.
Note: Sealing foils are also included in the LightCycler^® 480 Multiwell Plate 96, white, LightCycler^® 480 Multiwell Plate 96, clear, LightCycler^® 480 Multiwell Plate 384, white, and LightCycler^® 480 Multiwell Plate 384, clear packs.", "Country": "XG", "Code": "Content", "Name": "Content" }, { "Language": "en", "Value": "LightCycler^® 480 Sealing Foils prevent evaporation and contamination when running real-time PCR assays in LightCycler^® 480 Multiwell Plates (96 or 384 wells) using the LightCycler^® 480 Instrument.", "Country": "XG", "Code": "Applications", "Name": "Applications" }, { "Language": "en", "Value": "LightCycler^® 480 Sealing Foils are compatible with all types of LightCycler^® 480 Multiwell Plates (i.e., 96- and 384-well, white and clear). They are optically clear to facilitate qPCR and melting curve analysis based on fluorescence analysis.", "Country": "XG", "Code": "Product Description", "Name": "Product Description" } ] } } ] }