For general laboratory use. Others LightCycler EvoScript RNA Probes Master GLU LightCycler® EvoScript RNA Probes Master 3.5.9.5.3.0 LightCycler EvoScript RNA Probes Master 04038377029566 1 kit LC EvoScript RNA Probes Master (1000) 07800096001 1,000 reactions of 20 µl final volume each Reagents, kits en The LightCycler® EvoScript RNA Probes Master as a 1-vial composition, is designed for sensitive, high-specificity, high-precision one-step RT-qPCR especially for gene expression assays. en The LightCycler® EvoScript RNA Probes Master is designed for sensitive, high-specificity, high-precision one-step RT-qPCR especially for gene expression assays.The 1-vial composition is ideally suited for easy reaction assembly, requiring only the addition of primers, probe and target RNA. The proprietary reaction buffer allows a convenient hot start RT-qPCR. In addition, the enzymes for reverse transcription and for PCR feature hot start for premium specificity(1). The master mix is optimized for hydrolysis probes, including Universal ProbeLibrary (UPL) probes and does not require optimization with Mg(OAc)2.The reverse transcription activity will be suppressed at temperatures significantly below 60°C. en The LightCycler® EvoScript RNA Probes Master consists of 2 different vials:Vial 1: LightCycler® EvoScript RNA Probes Master, 5x conc.Vial 2: Water, PCR GradeThe kit also provides sufficient numbers of vials containing Water, PCR Grade to ensure that fresh (unopened) vials can be used. This minimizes the risk of contamination of RT-qPCR reaction mixes with RNases and other substances.The resulting RT-qPCR reaction mix also has a hot start Taq DNA Polymerase, nucleotides, and additives ensuring a hot start amplification system with high specificity. The mix contains an optimized concentration of Mg(OAc)2, eliminating the need for additional adjustments. For greater convenience, the RT-qPCR mixture can be used for both RNA and/or DNA templates in parallel.