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"en", "Value": "Store the kit at −15 to −25°C.", "Country": "XG", "Code": "Storage Conditions (Product)", "Name": "Storage Conditions (Product)" }, { "Language": "en", "Value": "The master mix is optimized with a fixed concentration of Mg(OAc)2, which works with nearly all primer combinations. There is no need for adjustment.", "Country": "XG", "Code": "Mg2+ Concentration", "Name": "Mg2+ Concentration" }, { "Language": "en", "Value": "The LightCycler® EvoScript RNA Probes Master as a 1-vial composition, is designed for sensitive, high-specificity, high-precision one-step RT-qPCR especially for gene expression assays.", "Country": "XG", "Code": "Product Description", "Name": "Product Description" }, { "Language": "en", "Value": "The LightCycler® EvoScript RNA Probes Master is designed for sensitive, high-specificity, high-precision one-step RT-qPCR especially for gene expression assays.
The 1-vial composition is ideally suited for easy reaction assembly, requiring only the addition of primers, probe and target RNA. The proprietary reaction buffer allows a convenient hot start RT-qPCR. In addition, the enzymes for reverse transcription and for PCR feature hot start for premium specificity(1). The master mix is optimized for hydrolysis probes, including Universal ProbeLibrary (UPL) probes and does not require optimization with Mg(OAc)2.
  1. The reverse transcription activity will be suppressed at temperatures significantly below 60°C.
", "Country": "XG", "Code": "Applications", "Name": "Applications" }, { "Language": "en", "Value": "The LightCycler® EvoScript RNA Probes Master consists of 2 different vials:
  • Vial 1: LightCycler® EvoScript RNA Probes Master, 5x conc.
  • Vial 2: Water, PCR Grade
The kit also provides sufficient numbers of vials containing Water, PCR Grade to ensure that fresh (unopened) vials can be used. This minimizes the risk of contamination of RT-qPCR reaction mixes with RNases and other substances.
The resulting RT-qPCR reaction mix also has a hot start Taq DNA Polymerase, nucleotides, and additives ensuring a hot start amplification system with high specificity. The mix contains an optimized concentration of Mg(OAc)2, eliminating the need for additional adjustments. For greater convenience, the RT-qPCR mixture can be used for both RNA and/or DNA templates in parallel.", "Country": "XG", "Code": "Principle", "Name": "Principle" }, { "Language": "en", "Value": "
Vial / BottleCapLabelFunction / DescriptionCatalog NumberContent
1blueLightCycler® EvoScript RNA Probes Master,
Master, 5x conc.
Contains enzymes for reverse
transcription and PCR,
RT-qPCR Reaction Buffer, dATP,
dCTP, dGTP, dUTP, Mg(OAc)2,
and proprietary additives.
07 800 029 0012 vials,
440 μl each
07 800 096 00110 vials,
440 μl each
2colorlessLightCycler® EvoScript RNA Probes Master,
Water, PCR Grade
To adjust the final reaction volume.07 800 029 0013 vials,
1 ml each
07 800 096 0012 vials,
7.25 ml each
", "Country": "XG", "Code": "Content", "Name": "Content" }, { "Language": "en", "Value": "90 minutes (includes a 15 minute reverse transcription step and 45 cycles of PCR)", "Country": "XG", "Code": "Assay Time", "Name": "Assay Time" } ] } } ] }

LightCycler® EvoScript RNA Probes Master

Easy-to-use reaction mix for one-step RT-qPCR using the LightCyler® 480 or the LightCycler® 96 Real-Time PCR Systems

LightCycler<sup><sup>®</sup></sup> EvoScript RNA Probes Master

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