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1. The reverse transcription activity will be suppressed at temperatures significantly below 60°C.

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• Vial 1: LightCycler^® EvoScript RNA Probes Master, 5x conc.
• Vial 2: Water, PCR Grade

The kit also provides sufficient numbers of vials containing Water, PCR Grade to ensure that fresh (unopened) vials can be used. This minimizes the risk of contamination of RT-qPCR reaction mixes with RNases and other substances.
The resulting RT-qPCR reaction mix also has a hot start Taq DNA Polymerase, nucleotides, and additives ensuring a hot start amplification system with high specificity. The mix contains an optimized concentration of Mg(OAc)₂, eliminating the need for additional adjustments. For greater convenience, the RT-qPCR mixture can be used for both RNA and/or DNA templates in parallel.", "Country": "XG", "Code": "Principle", "Name": "Principle" }, { "Language": "en", "Value": "

Vial / Bottle	Cap	Label	Function / Description	Catalog Number	Content
1	blue	LightCycler® EvoScript RNA Probes Master, Master, 5x conc.	Contains enzymes for reverse transcription and PCR, RT-qPCR Reaction Buffer, dATP, dCTP, dGTP, dUTP, Mg(OAc)2, and proprietary additives.	07 800 029 001	2 vials, 440 μl each
07 800 096 001	10 vials, 440 μl each
2	colorless	LightCycler® EvoScript RNA Probes Master, Water, PCR Grade	To adjust the final reaction volume.	07 800 029 001	3 vials, 1 ml each
07 800 096 001	2 vials, 7.25 ml each

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