{ "ProductData": { "RelatedLinks": "", "RegulatoryDisclaimer1": "For life science research only. Not for use in diagnostic procedures.", "RegulatoryDisclaimer2": "", "StainLocalization": [ "" ], "ProductType": "Others", "DisclaimerGroup2": null, "ProductNameGlobal": "LightCycler FastStart DNA MasterPLUS SYBR Green I", "DisclaimerGroup1": "LSR", "ControlTissue": [ "" ], "BrandName": "LightCycler® FastStart DNA MasterPLUS SYBR Green I", "LicenseDisclaimers": [ { "Order": "67", "Identifier": "1280837236338", "LongText": "SYBR Green I is manufactured by Molecular Probes, Inc., and is provided under license from Molecular Probes, Inc., for direct research use for PCR, where the dye is present during the PCR." } ], "Isotypes": "", "RegulatoryDisclaimer3": "", "ID": "3.5.5.1.3.4", "ProductNameAddition": "Easy-to-use hot start reaction mix for PCR using the LightCycler® Carousel-Based System", "Species": [ "" ], "Clone": "" }, "ProductImageDetails": { "ImagePath": "https://pim-media.roche.com/Images/Article_03752186001_im_en.png", "ImageType": "Image main" }, "Product2Taxomony": { "Product2TaxomonyReferences": [ { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeName": "LightCycler® Carousel-based System Kits", "NodeID": "06-0022" }, { "StructureSystemIdentifier": "OWP_Product_Types", "StructureSystemName": "Product Types", "NodeName": "Assays Reagents and Strips", "NodeID": "20-000-00" }, { "StructureSystemIdentifier": "OWP_Family", "StructureSystemName": "Product Families", "NodeName": "LightCycler", "NodeID": "230" }, { "StructureSystemIdentifier": "OWP_Techniques", "StructureSystemName": "Techniques", "NodeName": "PCR/qPCR", "NodeID": "210-00" }, { "StructureSystemIdentifier": "Product_Solutions", "StructureSystemName": "Product Solutions", "NodeName": "Molecular", "NodeID": "100" }, { "StructureSystemIdentifier": "Applications", "StructureSystemName": "Applications", "NodeName": "Gene Expression Analysis", "NodeID": "08-00-00" }, { "StructureSystemIdentifier": "Lab_Type", "StructureSystemName": "Lab Types", "NodeName": "LifeScience", "NodeID": "170-00" }, { "StructureSystemIdentifier": "Health_Topics", "StructureSystemName": "Health Topics", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Pathogens", "StructureSystemName": "Pathogens", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Disease_Areas", "StructureSystemName": "Disease Areas", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" } ] }, "Product2Taxonomy": { "Product2TaxonomyReferences": [ { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeName": "LightCycler® Carousel-based System Kits", "NodeID": "06-0022" }, { "StructureSystemIdentifier": "OWP_Product_Types", "StructureSystemName": "Product Types", "NodeName": "Assays Reagents and Strips", "NodeID": "20-000-00" }, { "StructureSystemIdentifier": "OWP_Family", "StructureSystemName": "Product Families", "NodeName": "LightCycler", "NodeID": "230" }, { "StructureSystemIdentifier": "OWP_Techniques", "StructureSystemName": "Techniques", "NodeName": "PCR/qPCR", "NodeID": "210-00" }, { "StructureSystemIdentifier": "Product_Solutions", "StructureSystemName": "Product Solutions", "NodeName": "Molecular", "NodeID": "100" }, { "StructureSystemIdentifier": "Applications", "StructureSystemName": "Applications", "NodeName": "Gene Expression Analysis", "NodeID": "08-00-00" }, { "StructureSystemIdentifier": "Lab_Type", "StructureSystemName": "Lab Types", "NodeName": "LifeScience", "NodeID": "170-00" }, { "StructureSystemIdentifier": "Health_Topics", "StructureSystemName": "Health Topics", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Pathogens", "StructureSystemName": "Pathogens", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Disease_Areas", "StructureSystemName": "Disease Areas", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" } ] }, "Product2Materials": { "P2MaterialReferences": [ { "MaterialNum": "03515869001", "MaterialDescription": "LC FS DNA Master PLUS SG, 96 react.", "RegisteredProductName": "LightCycler FastStart DNA MasterPLUS SYBR Green I", "GTIN": "04038377019611", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "1 kit", "PackSizeDescPIM360": "96 reactions of 20 μl final volume each", "MaterialAnnotation": "", "ReadyForUse": "false", "OrderInformation": "" }, { "MaterialNum": "03752186001", "MaterialDescription": "LC FS DNA MasterPLUS SG, 100 µl", "RegisteredProductName": "LightCycler FastStart DNA MasterPLUS SYBR Green I", "GTIN": "04038377020303", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "1 kit", "PackSizeDescPIM360": "1,920 reactions of 20 μl or 384 reactions of 100 μl final volume each", "MaterialAnnotation": "", "ReadyForUse": "false", "OrderInformation": "" }, { "MaterialNum": "03515885001", "MaterialDescription": "LC FS DNA Master PLUS SG, 480 react.", "RegisteredProductName": "LightCycler FastStart DNA MasterPLUS SYBR Green I", "GTIN": "04038377019628", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "1 kit", "PackSizeDescPIM360": "480 reactions of 20 μl final volume each", "MaterialAnnotation": "", "ReadyForUse": "false", "OrderInformation": "" } ] }, "Product2Products": { "Product2ProductReference": [ { "ProductID": "INS_409", "BrandName": "LightCycler® 2.0 Instrument", "ProductNameAddition": "", "ReferenceType": "Instrument", "Classification": [ { "IdentifierofStructureSystem": "1", "NameofStructureSystem": "GPCH", "StructureNodeID": "416", "StructureGroupPath": "Life Science->RLS qPCR & NAP Systems->LightCycler 2.0", "StructureGroupName": "LightCycler 2.0" }, { "IdentifierofStructureSystem": "OWP_Family", "NameofStructureSystem": "Product Families", "StructureNodeID": "230", "StructureGroupPath": "LightCycler", "StructureGroupName": "LightCycler" }, { "IdentifierofStructureSystem": "OWP_Product_Types", "NameofStructureSystem": "Product Types", "StructureNodeID": "10-000-00", "StructureGroupPath": "Analyzer Instruments and Systems", "StructureGroupName": "Analyzer Instruments and Systems" }, { "IdentifierofStructureSystem": "Lab_Type", "NameofStructureSystem": "Lab Types", "StructureNodeID": "150-00", "StructureGroupPath": "Research Lab", "StructureGroupName": "Research Lab" }, { "IdentifierofStructureSystem": "Product_Solutions", "NameofStructureSystem": "Product Solutions", "StructureNodeID": "100", "StructureGroupPath": "Molecular", "StructureGroupName": "Molecular" }, { "IdentifierofStructureSystem": "OWP_Techniques", "NameofStructureSystem": "Techniques", "StructureNodeID": "210-00", "StructureGroupPath": "PCR/qPCR", "StructureGroupName": "PCR/qPCR" }, { "IdentifierofStructureSystem": "OWP_Techniques", "NameofStructureSystem": "Techniques", "StructureNodeID": "300-00", "StructureGroupPath": "RT-PCR/qRT-PCR", "StructureGroupName": "RT-PCR/qRT-PCR" }, { "IdentifierofStructureSystem": "Applications", "NameofStructureSystem": "Applications", "StructureNodeID": "99-00-00", "StructureGroupPath": "NOT APPLICABLE", "StructureGroupName": "NOT APPLICABLE" }, { "IdentifierofStructureSystem": "Pathogens", "NameofStructureSystem": "Pathogens", "StructureNodeID": "99-00-00", "StructureGroupPath": "NOT APPLICABLE", "StructureGroupName": "NOT APPLICABLE" }, { "IdentifierofStructureSystem": "Disease_Areas", "NameofStructureSystem": "Disease Areas", "StructureNodeID": "99-00-00", "StructureGroupPath": "NOT APPLICABLE", "StructureGroupName": "NOT APPLICABLE" }, { "IdentifierofStructureSystem": "Health_Topics", "NameofStructureSystem": "Health Topics", "StructureNodeID": "99-00-00", "StructureGroupPath": "NOT APPLICABLE", "StructureGroupName": "NOT APPLICABLE" }, { "IdentifierofStructureSystem": "Product_Solutions", "NameofStructureSystem": "Product Solutions", "StructureNodeID": "055", "StructureGroupPath": "Laboratory developed testing", "StructureGroupName": "Laboratory developed testing" } ] } ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Language": "en", "Value": "Store the kit at −15 to −25°C.", "Country": "XG", "Code": "Storage Conditions (Product)", "Name": "Storage Conditions (Product)" }, { "Language": "en", "Value": "kit", "Country": "XG", "Code": "Form of Supply", "Name": "Form of Supply" }, { "Language": "en", "Value": "
ProcedureAssay Time
20 μl reactions [min]
Assay Time
100 μl reactions [min]
PCR Setup1515
LightCycler®Carousel-Based System PCR run (including Melting Curve)4590
Total Assay Time60105
", "Country": "XG", "Code": "Assay Time", "Name": "Assay Time" }, { "Language": "en", "Value": "LightCycler® FastStart DNA MasterPLUS SYBR Green I is an easy-to-use hot start reaction mix for highly sensitive PCR applications in LightCycler® Capillaries, using SYBR Green I as detection format, and an ideal master mix for performing two-step RT-PCR for gene expression analysis.
Note: LightCycler® FastStart DNA MasterPLUS SYBR Green I can be used in conjunction with heat-labile Uracil DNA Glycosylase for carryover prevention during PCR.", "Country": "XG", "Code": "Applications", "Name": "Applications" }, { "Language": "en", "Value": "During cycling, Fluorescence readings for SYBR Green I should be taken at the end of elongation. In the LightCycler® Software, the Acquisition Mode should be ‘Single’ in the Elongation/Extension step.

For Melting Curve, the Acquisition Mode can be ‘Continuous’ or ‘Step’.

In the ‘Continuous’ mode, fluorescence data is acquired continuously as the temperature changes, with the rate of data acquisition dependent on the number of capillaries.

In the ‘Step’ mode, fluorescence data is acquired at each temperature transition. For example, if the Ramp Rate is 0.5°C/sec, fluorescence data is acquired every 0.5°C for every capillary.



Channel Settings

For both online display and analysis of SYBR Green I, the channel setting should be 530.

For further information regarding Channel Settings, please refer to the LightCycler® Carousel-Based System LightCycler® Software 4.1 Addendum 1 - February 2010.



To perform Touchdown PCR with LightCycler® FastStart DNA MasterPLUS SYBR Green I, decrease the annealing temperature during the first 20 cycles by 10°C in steps of 2°C: start from an initial temperature +6 to +8°C above the melting temperature of your PCR primers down to a temperature 2°C above the final annealing temperature. Use a Ramp Rate of 0.5°C per cycle. The final 25 cycles are then run at the final annealing temperature.

An example touchdown protocol for a PCR primer set of Tm 56°C might be as follows:
95°C, 10 min 95°C, 10 s / 62°C 5 s / 72°C 10 s (x 4 cycles)
95°C, 10 s / 60°C 5 s / 72°C 10 s (x 4 cycles)
95°C, 10 s / 58°C 5 s / 72°C 10 s (x 4 cycles)
95°C, 10 s / 56°C 5 s / 72°C 10 s (x 4 cycles)
95°C, 10 s / 54°C 5 s / 72°C 10 s (x 4 cycles)
95°C, 10 s / 52°C 5 s / 72°C 10 s (x 25 cycles)

Note that elongation time depends on product length, follow the general rule: elongation time (sec) = amplicon length [bp]/25.



Sequencing of PCR products generated with SYBR Green I can be performed, after purifying with the High Pure PCR Product Purification Kit, to remove the SYBR Green I dye.



Cloning of PCR products amplified with the LightCycler® FastStart DNA MasterPLUS SYBR Green I may be done by direct T/A cloning, blunt-end cloning, or after restriction enzyme digestion. It is not necessary to purify the PCR products prior to cloning. As host, use a UNG-negative E. coli strain, as the LightCycler® Reagent Kits all contain dUTP.



LightCycler® FastStart DNA MasterPLUS SYBR Green I can be used for the study of hypermethylation. Please refer to the following articles:
  1. Florl, AR. et al. (2005). Screening for DNA Hypermethylation Using the LightCycler Instrument. Biochemica 1, 4-6.
  2. Thomassin, H. et al. (2004). MethylQuant: a sensitive method for quantifying methylation of specific cytosines within the genome. Nucleic Acids Res. 32, e168.



SYBR Green I is not suitable for detecting single point mutations.

SYBR Green I is most likely only suitable for the screening of large deletion mutations, because the SYBR Green I detection format can only resolve a single mutation in a 50 bp fragment. Customer data suggest that it is possible to detect small deletions/insertions (10 to 20 bp) with SYBR Green I. In general, Roche recommends the HybProbe Probe format for mutation analysis.", "Country": "XG", "Code": "Protocols - Help Corner", "Name": "Protocols - Help Corner" }, { "Language": "en", "Value": "
Vial / Bottle Cap Label Function/Description Catalog Number Content
1a white LC FastStart DNA MasterPLUS SYBR Green I, Enzyme Contains FastStart Taq DNA Polymerase, reaction buffer, MgCl2, SYBR Green I dye, and dNTP mix (with dUTP instead of dTTP). Ready-to-use hot start PCR Master after pipetting 14 μl from Vial 1a into one Vial 1b. 03 515 869 001 1 vial 1a and 3 vials 1b,
for 3 × 128 μl each each LC FastStart DNA MasterPLUS SYBR Green I, 5x conc.
03 515 885 001 5 vials 1a and 15 vials 1b,
for 15 × 128 μl each LC FastStart DNA MasterPLUS SYBR Green I, 5x conc.
1b green LC FastStart DNA MasterPLUS SYBR Green I, Reaction Mix Ready-to-use hot start PCR Master after pipetting 70 μl from Vial 1a into one Vial 1b. 03 752 186 001 4 vials 1a and 12 vials 1b,
for 12 × 640 μl each LC FastStart DNA MasterPLUS SYBR Green I, 5x conc.
2 colorless LC FastStart DNA MasterPLUS SYBR Green I, Water, PCR Grade To adjust the final reaction volume. 03 515 869 001 2 vials, 1 ml each
03 515 885 001 7 vials, 1 ml each
03 752 186 001 2 vials, 25 ml each
", "Country": "XG", "Code": "Content", "Name": "Content" }, { "Language": "en", "Value": "If precipitates are seen in the LightCycler® FastStart DNA MasterPLUS SYBR Green I, place the vial at +37°C and mix gently from time to time, until the precipitate is completely dissolved. This treatment does not influence the performance of PCR. Generally, precipitates should not be seen, as the buffer of the LightCycler® FastStart DNA MasterPLUS SYBR Green I was reformulated and optimized after the observation of precipitates in the LightCycler® FastStart DNA Master SYBR Green I.



Absolute fluorescence values in samples containing the same starting amount of template can vary. The most common reason is a difference in pipetting between samples. It is important to remember that absolute fluorescence readings for a sample are arbitrary and do not affect analysis of Cp (crossing point) determination or melting curve analysis in later data analysis.



During the seek process, the first capillary position is always found by the instrument, however, the capillaries thereafter must exceed a certain fluorescence background level (the \"seek threshold\") to be recognized by the instrument. With SYBR Green, the main reason why capillaries are not found during the initial seek process is that the fluorescent dye has either been omitted or degraded. To prevent degradation of the SYBR Green I dye, keep the LightCycler® FastStart DNA Master SYBR Green I away from light!", "Country": "XG", "Code": "Troubleshooting - Help Corner", "Name": "Troubleshooting - Help Corner" }, { "Language": "en", "Value": "Easy-to-use Hot Start Reaction Mix for PCR using SYBR Green I with the LightCycler® Carousel-Based System.", "Country": "XG", "Code": "Product Description", "Name": "Product Description" }, { "Language": "en", "Value": "Roche recommends the LightCycler® Probe Design Software 2.0 for designing PCR primers. This software provides precise estimations of the Tms of primers used with LightCycler® Reagent Kits.



Performing a Melting Curve analysis with SYBR Green I can identify and characterize PCR products based on their specific melting behavior. Moreover, non-specific products (primer-dimers) can also be identified using this method.



The principle of Melting Curve analysis is:
  1. At the beginning of a melting curve analysis, the reaction temperature is low and the fluorescence signal is high.
  2. As the temperature steadily increases, the fluorescence will suddenly drop as the reaction reaches the melting temperature (Tm) of each DNA fragment.
  3. A graph of melting behavior [plot of the first negative derivative (-dF/dT) of the fluorescence vs. temperature] reveals that pure, homogeneous PCR products produce a single, sharply defined melting curve with a narrow peak. In contrast, primer-dimers melt at relatively low temperatures and have broader peaks.



Crossing Point (Cp)
  1. In an amplification reaction, the cycle at which the fluorescence of a sample rises above the background fluorescence is called the \"crossing point\" (Cp) of the sample.
  2. The Cp of a sample appears as a sharp upward curve on the display of the experiment's fluorescence chart.
  3. The Cp is the point (PCR cycle number) at which the amplified product is first visible.
  4. A sample's Cp depends on the initial concentration of DNA in the sample. A sample with a lower initial concentration of target DNA requires more amplification cycles to reach the Cp. A sample with a higher concentration requires fewer cycles to produce the Cp.

The software uses the calculated Cps of the standard samples to generate the standard curve of Cp versus sample concentration.", "Country": "XG", "Code": "Background Information - Help Corner", "Name": "Background Information - Help Corner" }, { "Language": "en", "Value": "
Standard Laboratory Equipment
  • Nuclease-free, aerosol-resistant pipette tips
  • Pipettes with disposable, positive-displacement tips
  • Sterile reaction tubes for preparing master mixes and dilutions
For PCR
  • LightCycler® Carousel-Based System*
  • LightCycler® Capillaries*
    LightCycler® Capillaries (100 μl) can only be used with the LightCycler® 2.0 Instrument.
  • Standard benchtop microcentrifuge, containing a rotor for 2.0 ml reaction tubes.
The LightCycler® Carousel-Based System provides Centrifuge Adapters that enable LightCycler® Capillaries to be centrifuged in a standard microcentrifuge rotor.

or
  • LC Carousel Centrifuge 2.0* for use with the LightCycler® 2.0 Sample Carousel (optional)

  • LightCycler® Uracil-DNA Glycosylase* (optional)
 For prevention of carryover contamination see section Prevention of Carryover Contamination. Use LightCycler® Uracil-DNA Glycosylase in combination with LightCycler® FastStart DNA Masters only.
", "Country": "XG", "Code": "Additional Equipment and Reagent Required", "Name": "Additional Equipment and Reagent Required" }, { "Language": "en", "Value": "
Two-Step RT-PCR
LightCycler® FastStart DNA MasterPLUS SYBR Green I can also be used to perform two-step RT-PCR. In two-step RT-PCR, the reverse transcription of RNA into cDNA is separated from the other reaction steps and is performed outside the LightCycler® Carousel-Based System. Subsequent amplification and online monitoring is performed according to the standard LightCycler® Carousel-Based System procedure using the cDNA as starting sample material. One of the following reagents is required for reverse transcription of RNA into cDNA:
  • Transcriptor Reverse Transcriptase*
  • Transcriptor First Strand cDNA Synthesis Kit*

Synthesis of cDNA is performed according to the detailed instructions provided with the cDNA synthesis reagent.

Do not use more than 8 μl of undiluted cDNA template per 20 μl final reaction volume, because greater amounts may inhibit PCR. For initial experiments, we recommend running undiluted, 1:10 diluted, and 1:100 diluted cDNA template in parallel to determine the optimal template amount.
", "Country": "XG", "Code": "General Considerations", "Name": "General Considerations" }, { "Language": "en", "Value": "Generation of PCR products can be detected by measurement of the SYBR Green I fluorescence signal. SYBR Green I intercalates into the DNA double helix. In solution, the unbound SYBR Green I dye exhibits very little fluorescence; however, fluorescence (wavelength, 530 nm) is greatly enhanced upon DNA binding. Therefore, during PCR, the increase in SYBR Green I fluorescence is proportional to the amount of double-stranded DNA generated.
As SYBR Green I dye is very stable (only 6% of the activity is lost during 30 amplification cycles) and the LightCycler® Instruments' optical filter set matches the wavelengths of excitation and emission.
The basic steps of DNA detection by SYBR Green I during real-time PCR on the LightCycler® Carousel-Based System are:
  1. At the beginning of amplification, the reaction mixture contains the denatured DNA, the primers, and the SYBR Green I dye. The unbound SYBR Green I dye molecules weakly fluoresce, producing a minimal background fluorescence signal, which is subtracted during computer analysis.
  2. After annealing of the primers, a few SYBR Green I dye molecules can bind to the double strand. DNA binding results in a dramatic increase of the SYBR Green I dye molecules to emit light upon excitation.
  3. During elongation, more and more SYBR Green I dye molecules bind to the newly synthesized DNA. If the reaction is monitored continuously, an increase in fluorescence is viewed in real time. Upon denaturation of the DNA for the next heating cycle, the SYBR Green I dye molecules are released and the fluorescence signal falls.
  4. Fluorescence measurement at the end of the elongation step of every PCR cycle is performed to monitor the increasing amount of amplified DNA.
     
To demonstrate that only your desired PCR product has been amplified, you may perform a Melting Curve analysis after PCR. In Melting Curve analysis, the reaction mixture is slowly heated to +95°C, which causes melting of double-stranded DNA and a corresponding decrease of SYBR Green I fluorescence. The Instrument continuously monitors this fluorescence decrease and displays it as melting peaks. Each melting peak represents the characteristic melting temperature (Tm) of a particular DNA product (where the DNA is 50% double-stranded and 50% single-stranded). The most important factors that determine the Tm of dsDNA are the length and the GC-content of that fragment. If PCR generated only one amplicon, Melting Curve analysis will show only one melting peak. If primer-dimers or other nonspecific products are present, they will be shown as additional melting peaks. Checking the Tm of a PCR product can thus be compared with analyzing a PCR product by length in gel electrophoresis.

How this Product Works
LightCycler® FastStart DNA MasterPLUS SYBR Green I is a ready-to-use PCR reaction mix, designed specifically for real-time PCR assays using the SYBR Green I detection format on the LightCycler® Carousel-Based System. It can be used to perform hot start PCR in 20 or 100 μl glass capillaries. Hot start PCR has been shown to significantly improve specificity and sensitivity of PCR (Chou, Q., et al., 1992; Kellogg, D.E., et al., 1994; Birch, D.E., et al., 1996), by minimizing the formation of nonspecific amplification products at the beginning of the reaction.
FastStart Taq DNA Polymerase is a chemically modified form of thermostable recombinant Taq DNA polymerase, that shows no activity up to 75°C. The enzyme is active only at high temperatures, where primers no longer bind nonspecifically. The enzyme is completely activated (by removal of blocking groups) in a single pre-incubation step (95°C, 10 minutes) before cycling begins. Activation does not require the extra handling steps typical of other hot start techniques.
The composition of the reaction mix is optimized for a fixed MgCl2 concentration. This kit achieves an efficient amplification with almost all primer combinations, without any sequence specific optimization.
LightCycler® FastStart DNA MasterPLUS SYBR Green I provides convenience, high performance, and reproducibility, as well as minimizing contamination risk. All you need to supply is primers and template DNA.

In combination with the LightCycler® Carousel-Based System and suitable PCR primers, this kit enables very sensitive detection and quantification of defined DNA sequences. Furthermore, the kit can be used to perform two-step RT-PCR, in combination with a reverse transcription kit for cDNA synthesis.

The amplicon size should not exceed 1 kb in length. For optimal results, select a product length of 700 bp or less.
", "Country": "XG", "Code": "Principle", "Name": "Principle" } ] } } ] }

LightCycler® FastStart DNA MasterPLUS SYBR Green I

Easy-to-use hot start reaction mix for PCR using the LightCycler® Carousel-Based System

LightCycler<sup><sup>®</sup></sup> FastStart DNA Master<sup>PLUS</sup> SYBR Green I

Ordering Information

Overview

Technical Documents

Access Material Data Sheets, Certificates of Analysis, and other product documentation.

After clicking above, you will be redirected to eLabDoc, where you can choose your local country.

Instruments

...
    ...
    error errorMessage
    Sorry, we couldn't find the content you are looking for
    Please try again later