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Spectral Characteristics
An oligonucleotide labeled with LightCycler® Fluorescein shows an excitation maximum at 494 nm and an emission maximum at 519 nm (both measured in 2 mM Tris buffer, pH 8.3).

Fig. 3: Excitation and emission spectra of an oligonucleotide which is labeled with LightCycler® Fluorescein.
Labeling Principle
LightCycler® Fluorescein CPG contains a dimethoxytrityl-protected hydroxyl function which makes it suitable for elongation with standard phosphoramidites after conventional deprotection dring the first synthesis cycle.

 ", "Language": "en", "Country": "XG", "Code": "Principle" }, { "Name": "Product Description", "Value": "Fluorescein long chain alkylamino Controlled Pore Glass (lcaa CPG, 500 Å) for labeling the 3' terminus of oligonucleotides with Fluorescein.
Biochemica - LightCycler™ Hybridization Probes - 1999", "Language": "en", "Country": "XG", "Code": "Product Description" }, { "Name": "Applications", "Value": "LightCycler® Fluorescein CPG labels the 3' end of an oligonucleotide that is combined with a 5' LightCycler® Red-labeled oligonucleotide when using HybProbe probes for sequence-specific detection in real-time PCR.
This support can be used in the same manner as standard nucleoside supports since it contains a dimethoxytrityl group.", "Language": "en", "Country": "XG", "Code": "Applications" }, { "Name": "Content", "Value": "
Vial/Bottle Label Function / Description Content
1 LightCycler® Fluorescein CPG
  • Contains LightCycler® Fluorescein long chain alkylamino Controlled Pore Glass (lcaa CPG) 500 Å for labeling of oligonucleotides at the 3' end.

  • The reagent is supplied as a white solid. The loading of the CPG material is printed on the label.
1 bottle, 1 g
", "Language": "en", "Country": "XG", "Code": "Content" } ] } } ] }

LightCycler® Fluorescein CPG

For the synthesis of oligonucleotides labeled with fluorescein at the 3' end

LSR For life science research only. Not for use in diagnostic procedures.
LightCycler<sup>®</sup> Fluorescein CPG

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