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An oligonucleotide labeled with LightCycler® Fluorescein shows an excitation maximum at 494 nm and an emission maximum at 519 nm (both measured in 2 mM Tris buffer, pH 8.3).
Fig. 3: Excitation and emission spectra of an oligonucleotide which is labeled with LightCycler® Fluorescein.
Labeling Principle
LightCycler® Fluorescein CPG contains a dimethoxytrityl-protected hydroxyl function which makes it suitable for elongation with standard phosphoramidites after conventional deprotection dring the first synthesis cycle.
",
"Language": "en",
"Country": "XG",
"Code": "Principle"
},
{
"Name": "Product Description",
"Value": "Fluorescein long chain alkylamino Controlled Pore Glass (lcaa CPG, 500 Å) for labeling the 3' terminus of oligonucleotides with Fluorescein. Biochemica - LightCycler™ Hybridization Probes - 1999",
"Language": "en",
"Country": "XG",
"Code": "Product Description"
},
{
"Name": "Applications",
"Value": "LightCycler® Fluorescein CPG labels the 3' end of an oligonucleotide that is combined with a 5' LightCycler® Red-labeled oligonucleotide when using HybProbe probes for sequence-specific detection in real-time PCR. This support can be used in the same manner as standard nucleoside supports since it contains a dimethoxytrityl group.",
"Language": "en",
"Country": "XG",
"Code": "Applications"
},
{
"Name": "Content",
"Value": "
Vial/Bottle
Label
Function / Description
Content
1
LightCycler® Fluorescein CPG
Contains LightCycler® Fluorescein long chain alkylamino Controlled Pore Glass (lcaa CPG) 500 Å for labeling of oligonucleotides at the 3' end.
The reagent is supplied as a white solid. The loading of the CPG material is printed on the label.