{ "ProductData": { "ID": "3.5.5.2.1.5", "ProductType": "Others", "BrandName": "LightCycler^® Fluorescein CPG", "ProductNameAddition": "For the synthesis of oligonucleotides labeled with fluorescein at the 3' end", "RegulatoryDisclaimer1": "For life science research only. Not for use in diagnostic procedures.", "DisclaimerGroup1": "LSR", "RegulatoryDisclaimer2": "", "DisclaimerGroup2": null, "RegulatoryDisclaimer3": "", "SampleType": [ ], "LicenseDisclaimers": [ { "Order": "15", "Identifier": "70051125019", "LongText": "This product is sold for use by purchaser only. The sale or distribution of this product or any product derived from the use of this product to any third party is expressly excluded. Especially excluded is the commercial use or sale of oligonucleotides or other products labeled with this product." } ], "RelatedLinks": "", "Clone": "", "ControlTissue": [ "" ], "ISOtypes": "", "Species": [ "" ], "StainLocalization": [ "" ], "ProductNameGlobal": "LightCycler Fluorescein CPG" }, "ProductImageDetails": { "ImagePath": "https://pim-media.roche.com/Images/Article_03138178001_im_en.png", "ImageType": "Image main" }, "Product2Taxonomy": { "Product2TaxonomyReferences": [ { "StructureSystemIdentifier": "OWP_Product_Types", "StructureSystemName": "Product Types", "StructureNodeStatus": "valid", "NodeName": "Auxiliaries", "NodeID": "95-000-00" }, { "StructureSystemIdentifier": "OWP_Family", "StructureSystemName": "Product Families", "StructureNodeStatus": "valid", "NodeName": "LightCycler", "NodeID": "230" }, { "StructureSystemIdentifier": "Lab_Type", "StructureSystemName": "Lab Types", "StructureNodeStatus": "valid", "NodeName": "Research Lab", "NodeID": "150-00" }, { "StructureSystemIdentifier": "Product_Solutions", "StructureSystemName": "Product Solutions", "StructureNodeStatus": "valid", "NodeName": "Molecular", "NodeID": "100" }, { "StructureSystemIdentifier": "OWP_Techniques", "StructureSystemName": "Techniques", "StructureNodeStatus": "valid", "NodeName": "Labeling and Detection", "NodeID": "320-00" }, { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "StructureNodeStatus": "valid", "NodeName": "LightCycler® Carousel-based System Kits", "NodeID": "06-0022" }, { "StructureSystemIdentifier": "Applications", "StructureSystemName": "Applications", "StructureNodeStatus": "valid", "NodeName": "Probe Labeling", "NodeID": "33-00-00" }, { "StructureSystemIdentifier": "Lab_Type", "StructureSystemName": "Lab Types", "StructureNodeStatus": "invalid", "NodeName": "", "NodeID": "170-00" }, { "StructureSystemIdentifier": "Disease_Areas", "StructureSystemName": "Disease Areas", "StructureNodeStatus": "invalid", "NodeName": "", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Pathogens", "StructureSystemName": "Pathogens", "StructureNodeStatus": "invalid", "NodeName": "", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Health_Topics", "StructureSystemName": "Health Topics", "StructureNodeStatus": "invalid", "NodeName": "", "NodeID": "99-00-00" } ] }, "Product2Materials": { "P2MaterialReferences": [ { "MaterialNum": "03138178001", "MaterialDescription": "LC Fluorescein CPG, 1g", "RegisteredProductName": "LightCycler Fluorescein CPG", "GTIN": "04038377017334", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "1 g", "PackSizeDescPIM360": "Not Available", "MaterialAnnotation": "", "ReadyForUse": "", "OrderInformation": "" } ] }, "Product2Products": { "Product2ProductReference": [ { "ProductID": "INS_409", "BrandName": "LightCycler^® 2.0 Instrument", "ProductNameAddition": "", "ReferenceType": "Instrument", "Classification": [ { "IdentifierofStructureSystem": "1", "NameofStructureSystem": "GPCH", "StructureNodeID": "416", "StructureGroupPath": "", "StructureGroupName": "", "StructureNodeStatus": "" }, { "IdentifierofStructureSystem": "OWP_Family", "NameofStructureSystem": "Product Families", "StructureNodeID": "230", "StructureGroupPath": "LightCycler", "StructureGroupName": "LightCycler", "StructureNodeStatus": "valid" }, { "IdentifierofStructureSystem": "OWP_Product_Types", "NameofStructureSystem": "Product Types", "StructureNodeID": "10-000-00", "StructureGroupPath": "Analyzer Instruments and Systems", "StructureGroupName": "Analyzer Instruments and Systems", "StructureNodeStatus": "valid" }, { "IdentifierofStructureSystem": "Lab_Type", "NameofStructureSystem": "Lab Types", "StructureNodeID": "150-00", "StructureGroupPath": "Research Lab", "StructureGroupName": "Research Lab", "StructureNodeStatus": "valid" }, { "IdentifierofStructureSystem": "Product_Solutions", "NameofStructureSystem": "Product Solutions", "StructureNodeID": "100", "StructureGroupPath": "Molecular", "StructureGroupName": "Molecular", "StructureNodeStatus": "valid" }, { "IdentifierofStructureSystem": "OWP_Techniques", "NameofStructureSystem": "Techniques", "StructureNodeID": "210-00", "StructureGroupPath": "PCR/qPCR", "StructureGroupName": "PCR/qPCR", "StructureNodeStatus": "valid" }, { "IdentifierofStructureSystem": "OWP_Techniques", "NameofStructureSystem": "Techniques", "StructureNodeID": "300-00", "StructureGroupPath": "RT-PCR/qRT-PCR", "StructureGroupName": "RT-PCR/qRT-PCR", "StructureNodeStatus": "valid" }, { "IdentifierofStructureSystem": "Applications", "NameofStructureSystem": "Applications", "StructureNodeID": "99-00-00", "StructureGroupPath": "", "StructureGroupName": "", "StructureNodeStatus": "invalid" }, { "IdentifierofStructureSystem": "Pathogens", "NameofStructureSystem": "Pathogens", "StructureNodeID": "99-00-00", "StructureGroupPath": "", "StructureGroupName": "", "StructureNodeStatus": "invalid" }, { "IdentifierofStructureSystem": "Disease_Areas", "NameofStructureSystem": "Disease Areas", "StructureNodeID": "99-00-00", "StructureGroupPath": "", "StructureGroupName": "", "StructureNodeStatus": "invalid" }, { "IdentifierofStructureSystem": "Health_Topics", "NameofStructureSystem": "Health Topics", "StructureNodeID": "99-00-00", "StructureGroupPath": "", "StructureGroupName": "", "StructureNodeStatus": "invalid" }, { "IdentifierofStructureSystem": "Product_Solutions", "NameofStructureSystem": "Product Solutions", "StructureNodeID": "055", "StructureGroupPath": "Laboratory developed testing", "StructureGroupName": "Laboratory developed testing", "StructureNodeStatus": "valid" } ] } ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Language": "en", "Value": "Store the product at −15 to −25°C.", "Country": "XG", "Code": "Storage Conditions (Product)", "Name": "Storage Conditions (Product)" }, { "Language": "en", "Value": "An oligonucleotide labeled with LightCycler^® Fluorescein shows an excitation maximum at 494 nm and an emission maximum at 519 nm (both measured in 2 mM Tris buffer, pH 8.3).

Fig. 1: Emission spectra of fluorescent dyes.LightCycler^® Fluorescein CPG contains a dimethoxytrityl-protected hydroxyl function which makes it suitable for elongation with standard phosphoramidites after conventional deprotection dring the first synthesis cycle.

 ", "Country": "XG", "Code": "Principle", "Name": "Principle" }, { "Language": "en", "Value": "Fluorescein long chain alkylamino Controlled Pore Glass (lcaa CPG, 500 Å) for labeling the 3' terminus of oligonucleotides with Fluorescein.
Biochemica - LightCycler™ Hybridization Probes - 1999", "Country": "XG", "Code": "Product Description", "Name": "Product Description" }, { "Language": "en", "Value": "LightCycler^® Fluorescein CPG labels the 3' end of an oligonucleotide that is combined with a 5' LightCycler^® Red-labeled oligonucleotide when using HybProbe probes for sequence-specific detection in real-time PCR.
This support can be used in the same manner as standard nucleoside supports since it contains a dimethoxytrityl group.", "Country": "XG", "Code": "Applications", "Name": "Applications" }, { "Language": "en", "Value": "

Vial/Bottle	Label	Function / Description	Content
1	LightCycler® Fluorescein CPG	Contains LightCycler® Fluorescein long chain alkylamino Controlled Pore Glass (lcaa CPG) 500 Å for labeling of oligonucleotides at the 3' end. The reagent is supplied as a white solid. The loading of the CPG material is printed on the label.	1 bottle, 1 g

", "Country": "XG", "Code": "Content", "Name": "Content" } ] } } ] }