For life science research only. Not for use in diagnostic procedures. Others LightCycler Fluorescein CPG LSR LightCycler® Fluorescein CPG 15 70051125019 This product is sold for use by purchaser only. The sale or distribution of this product or any product derived from the use of this product to any third party is expressly excluded. Especially excluded is the commercial use or sale of oligonucleotides or other products labeled with this product. 3.5.5.2.1.5 03138178001 LC Fluorescein CPG, 1g LightCycler Fluorescein CPG 04038377017334 Reagents, kits 1 g Not Available Spectral CharacteristicsAn oligonucleotide labeled with LightCycler® Fluorescein shows an excitation maximum at 494 nm and an emission maximum at 519 nm (both measured in 2 mM Tris buffer, pH 8.3).Fig. 3: Excitation and emission spectra of an oligonucleotide which is labeled with LightCycler® Fluorescein.Labeling PrincipleLightCycler® Fluorescein CPG contains a dimethoxytrityl-protected hydroxyl function which makes it suitable for elongation with standard phosphoramidites after conventional deprotection dring the first synthesis cycle.  en Fluorescein long chain alkylamino Controlled Pore Glass (lcaa CPG, 500 Å) for labeling the 3' terminus of oligonucleotides with Fluorescein.Biochemica - LightCycler™ Hybridization Probes - 1999 en LightCycler® Fluorescein CPG labels the 3' end of an oligonucleotide that is combined with a 5' LightCycler® Red-labeled oligonucleotide when using HybProbe probes for sequence-specific detection in real-time PCR.This support can be used in the same manner as standard nucleoside supports since it contains a dimethoxytrityl group. en