For general laboratory use. Others LightCycler Multiplex RNA Virus Master GLU LightCycler® Multiplex RNA Virus Master 3.5.9.5.2.7 06754155001 LC Multiplex RNA Virus Master, 200 LightCycler Multiplex RNA Virus Master 04038377028545 Reagents, kits 1 kit 200 reactions of 20 μL final volume each false 07083173001 LC Multiplex RNA Virus Master, 1000 LightCycler Multiplex RNA Virus Master 04038377029054 Reagents, kits 1 kit 1,000 reactions of 20 μL final volume each false The LightCycler® Multiplex RNA Virus Master is designed for fast, highly sensitive and specific real-time one-step RT-qPCR analysis of viral RNA.The 2-vial composition (separate RT enzyme and qPCR Mix) is ideally suited for use with RT-minus controls. The proprietary reaction buffer allows a fast and convenient hot start RT-qPCR without pre-activation of the Taq DNA Polymerase. The kit is optimized for hydrolysis probes and does not require optimization with MgCl2. en How this product worksThe LightCycler® Multiplex RNA Virus Master consists of 3 different vials:Vial 1: RT Enzyme Solution (Reverse Transcriptase)Vial 2: RT-qPCR Reaction MixVial 3: Water, PCR GradeThe separate vial of Reverse Transcriptase makes it possible to prepare a RT-qPCR reaction mix for running RT-minus controls (lacking reverse transcriptase). This is important for verifying whether the obtained results are derived from RNA transcripts or from residual (contaminating) genomic DNA. The kit also provides sufficient vials of Water, PCR Grade to ensure that fresh (unopened) vials can be used. This minimizes the risk of contamination of RT-qPCR reaction mixes by RNases and other substances.The Reverse Transcriptase provided in this kit is a recombinant reverse transcriptase with higher stability than native reverse transcriptase. This feature allows for higher reverse transcription temperatures of up to +55°C. This Roche recombinant Reverse Transcriptase also has lower affinity for DNA than other commonly used reverse transcriptases.The resulting RT-qPCR reaction mix also has AptaTaq DNA Polymerase, nucleotides, and additives, ensuring a hot start amplification system with high specificity. The mix contains an optimized concentration of MgCl2, eliminating the need for additional adjustments. For greater convenience, the RT-qPCR mixture, including Reverse Transcriptase, can be used for both RNA and/or DNA templates in parallel. en