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For example, a septaplex protocol using 45 cycles requires less than 75 minutes when using the LightCycler^® PRO System.", "Language": "en", "Country": "XG", "Code": "Typical run time" }, { "Name": "Storage Conditions (Product)", "Value": "Store the kit at −15 to −25°C.", "Language": "en", "Country": "XG", "Code": "Storage Conditions (Product)" }, { "Name": "Applications", "Value": "The LightCycler^® Multiplex RNA Virus Master is designed for fast, highly sensitive and specific real-time one-step RT-qPCR analysis of viral RNA.
The 2-vial composition (separate RT enzyme and qPCR Mix) is ideally suited for use with RT-minus controls. The proprietary reaction buffer allows a fast and convenient hot start RT-qPCR without pre-activation of the Taq DNA Polymerase. The kit is optimized for hydrolysis probes and does not require optimization with MgCl₂.", "Language": "en", "Country": "XG", "Code": "Applications" }, { "Name": "Additional Equipment and Reagent Required", "Value": "

• Nuclease-free pipette tips
• 1.5 mL RNase-free microcentrifuge tubes to prepare master mixes and dilutions
• To minimize risk of RNase contamination, autoclave all vessels
  Wear gloves at all times.

• Real-Time PCR systems such as the LightCycler^® PRO, LightCycler^® 480, LightCycler^® 96, LightCycler^® 2.0 Systems*, or other Real-Time PCR Systems
• LightCycler^® 480 Multiwell Plate 96, white*
• LightCycler^® 480 Multiwell Plate 384, white*
• LightCycler^® 480 Multiwell Plate 96, white, 4 bar codes*
• LightCycler^® 480 Multiwell Plate 384, white, 4 bar codes*
• Sealing Foil Applicator*
• LightCycler^® 480 Sealing Foil*
• LightCycler^® 8-Tube Strips*
• LightCycler^® 8-Tube Strip Adapter Plate*
• Centrifuge with swinging-bucket rotor

", "Language": "en", "Country": "XG", "Code": "Additional Equipment and Reagent Required" }, { "Name": "Content", "Value": "

Vial / Bottle	Cap	Label	Function / Description	Catalog Number	Content
1	blue	LightCycler® Multiplex RNA Virus Master, RT Enzyme Solution, 200x conc.	Contains Reverse Transcriptase.	06 754 155 001	1 vial, 28 μL
07 083 173 001	5 vials, 28 μL each
2	red	LightCycler® Multiplex RNA Virus Master, RT-qPCR Reaction Mix, 5x conc.	Contains RT-qPCR Reaction Buffer, AptaTaq Polymerase, dATP, dCTP, dGTP, dUTP, MgCl2, and proprietary additives.	06 754 155 001	1 vial, 880 μL
07 083 173 001	5 vials, 880 μL each
3	colorless	LightCycler® Multiplex RNA Virus Master, Water, PCR Grade	To adjust the final reaction volume.	06 754 155 001	3 vials, 1 mL each
07 083 173 001	15 vials, 1 mL each

", "Language": "en", "Country": "XG", "Code": "Content" }, { "Name": "Principle", "Value": "The LightCycler^® Multiplex RNA Virus Master consists of 3 different vials:

• Vial 1: RT Enzyme Solution (Reverse Transcriptase)
• Vial 2: RT-qPCR Reaction Mix
• Vial 3: Water, PCR Grade

The separate vial of Reverse Transcriptase makes it possible to prepare a RT-qPCR reaction mix for running RT-minus controls (lacking reverse transcriptase). This is important for verifying whether the obtained results are derived from RNA transcripts or from residual (contaminating) genomic DNA. The kit also provides sufficient vials of Water, PCR Grade to ensure that fresh (unopened) vials can be used. This minimizes the risk of contamination of RT-qPCR reaction mixes by RNases and other substances.
The Reverse Transcriptase provided in this kit is a recombinant reverse transcriptase with higher stability than native reverse transcriptase. This feature allows for higher reverse transcription temperatures of up to +55°C. This Roche recombinant Reverse Transcriptase also has lower affinity for DNA than other commonly used reverse transcriptases.
The resulting RT-qPCR reaction mix also has AptaTaq DNA Polymerase, nucleotides, and additives, ensuring a hot start amplification system with high specificity. The mix contains an optimized concentration of MgCl₂, eliminating the need for additional adjustments. For greater convenience, the RT-qPCR mixture, including Reverse Transcriptase, can be used for both RNA and/or DNA templates in parallel.", "Language": "en", "Country": "XG", "Code": "Principle" }, { "Name": "Product Purpose", "Value": "The LightCycler^® Multiplex RNA Virus Master is designed for fast, highly sensitive and specific real-time one-step RT-qPCR analysis of viral RNA.
The 2-vial composition (separate RT enzyme and qPCR Mix) is ideally suited for use with RT-minus controls. The proprietary reaction buffer allows a fast and convenient hot start RT-qPCR without pre-activation of the Taq DNA Polymerase. The kit is optimized for hydrolysis probes and does not require optimization with MgCl₂.", "Language": "en", "Country": "XG", "Code": "Product Purpose" } ] } } ] }