For general laboratory use. Others LightCycler PCR QC Kit GLU LightCycler® PCR QC Kit 67 1280837236338 SYBR Green I is manufactured by Molecular Probes, Inc., and is provided under license from Molecular Probes, Inc., for direct research use for PCR, where the dye is present during the PCR. 3.8.1.7.4.1 LightCycler PCR QC Kit 04038377028637 1 kit LightCycler PCR QC Kit 06746381001 for 1 test runUp to 96 reactions with 10 μL final volume each Reagents, kits en The LightCycler® PCR QC Kit contains all reagents for a test run to verify the correct function of a LightCycler® PRO or LightCycler® 96 System.The SYBR Green I detection format is used to perform amplification and melting curve analysis. The test is comprised of 48 or 96 reactions to determine temperature accuracy across the entire block by calculating Tm values. Potential instrument effects on PCR results using Cq values are also measured. In addition, there is a check for fluorescence intensity by precisely monitoring the initial fluorescence between cycles 1 to 17.The LightCycler® PCR QC Kit uses the FastStart Essential DNA Green Master, a ready-to-use reaction mix for hot start PCR in the SYBR Green I detection format. This master mix includes FastStart Taq Polymerase, a chemically modified thermostable recombinant Taq DNA Polymerase, showing no activity up to +75°C. This recombinant enzyme is active only at high temperatures at which primers can no longer bind nonspecifically. The enzyme is completely activated by removal of blocking groups in a single pre-incubation step (+95°C, 10 minutes) before PCR cycling begins. Activation does not require the extra handling steps typical of other hot start techniques. en Kit for Quality Control of the LightCycler® PRO or LightCycler® 96 Systems.