For life science research only. Not for use in diagnostic procedures. Others LightCycler Red 610-N-hydroxysuccinimide ester LSR LightCycler® Red 610-N-hydroxysuccinimide ester 15 70051125019 This product is sold for use by purchaser only. The sale or distribution of this product or any product derived from the use of this product to any third party is expressly excluded. Especially excluded is the commercial use or sale of oligonucleotides or other products labeled with this product. 3.5.5.2.1.6 03561488001 LC Red 610 N hydroxysuccinimide ester LightCycler Red 610-N-hydroxysuccinimide ester 04038377020280 Reagents, kits 1 vial for 5 x 50 nmol oligonucleotides The LightCycler® Red 610-NHS ester is used for labeling the 5' end of the downstream probe when HybProbe Probes are used as the detection format with the respective LightCycler® System. In addition, the 3' end of the probe must be phosphorylated to avoid elongation during PCR. en Spectral CharacteristicsFig. 3: LightCycler® Red 610 shows an excitation maximum at 590 nm and an emission maximum at 610 nm (in 2 mM Tris buffer, pH 8.3).Labeling Principle5'-amino-substituted-3'-phosphorylated oligonucleotides react with the LightCycler® Red 610-NHS ester in a sodium borate buffer/dimethylformamide (DMF) mixture at pH 8.5 - 9.0. en Hydroxysuccinimide ester for labeling the 5' amino terminus of oligonucleotides with LightCycler® Red 610. en