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Vial/Bottle Label Function/Description Content
1 LightCycler® Red 610 NHS-ester for ≥250 nmol oligonuc.
  • Contains a sufficient amount of LightCycler® Red 610-NHS ester for labeling a minimum of 5 × 50 nmol oligonucleotide.
  • The reagent is supplied as a blue solid.
1 vial
", "Country": "XG", "Code": "Content", "Name": "Content" }, { "Language": "en", "Value": "
Spectral Characteristics
Fig. 1: Emission spectra of fluorescent dyes.

Labeling Principle
5'-amino-substituted-3'-phosphorylated oligonucleotides react with the LightCycler® Red 610-NHS ester in a sodium borate buffer/dimethylformamide (DMF) mixture at pH 8.5 - 9.0.", "Country": "XG", "Code": "Principle", "Name": "Principle" }, { "Language": "en", "Value": "Hydroxysuccinimide ester for labeling the 5' amino terminus of oligonucleotides with LightCycler® Red 610.", "Country": "XG", "Code": "Product Description", "Name": "Product Description" }, { "Language": "en", "Value": "
For Oligonucleotide Labeling
  • DNA synthesizer
  • Standard reagents for oligonucleotide synthesis (tetrazol, etc.)
  • Absolute, amine-free dimethylformamide (DMF)
  • 5'-Amino modifier
  • 3'-Phosphate CPG support
  • Standard phosphoramidites
  • 0.1 M sodium borate buffer (pH 8.5)

For Ethanol Precipitation of Oligonucleotide
  • 3 M sodium acetate buffer (pH 8.5)
  • Ice-cold absolute ethanol

For Oligonucleotide Purification by HPLC
  • HPLC
  • Vacuum centrifuge
  • POROS OligoR3 separation medium (PerSeptive Biosystems, Inc., 4.6 × 50 mm column). This separation medium is recommended to obtain optimal purification results.
  • 100 mM Triethylammoniumacetate (pH 6.9)
  • Acetonitrile
Do not use Reversed Phase (RP) separation media because labeled oligonucleotides tend to stick irreversibly to the RP-material, resulting in lower yields of labeled oligonucleotides.
For Quality Control of HPLC-Purified Oligonucleotides
  • Photometer
", "Country": "XG", "Code": "Additional Equipment and Reagent Required", "Name": "Additional Equipment and Reagent Required" } ] } } ] }

LightCycler® Red 610-N-hydroxysuccinimide ester

For labeling a minimum of 5 × 50 nmol oligonucleotides

LightCycler<sup><sup>®</sup></sup> Red 610-N-hydroxysuccinimide ester

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