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Fig. 3: LightCycler® Red 610 shows an excitation maximum at 590 nm and an emission maximum at 610 nm (in 2 mM Tris buffer, pH 8.3).
Labeling Principle
5'-amino-substituted-3'-phosphorylated oligonucleotides react with the LightCycler® Red 610-NHS ester in a sodium borate buffer/dimethylformamide (DMF) mixture at pH 8.5 - 9.0.",
"Country": "XG",
"Code": "Principle",
"Name": "Principle"
},
{
"Language": "en",
"Value": "Hydroxysuccinimide ester for labeling the 5' amino terminus of oligonucleotides with LightCycler® Red 610.",
"Country": "XG",
"Code": "Product Description",
"Name": "Product Description"
},
{
"Language": "en",
"Value": "
For Oligonucleotide Labeling
DNA synthesizer
Standard reagents for oligonucleotide synthesis (tetrazol, etc.)
Absolute, amine-free dimethylformamide (DMF)
5'-Amino modifier
3'-Phosphate CPG support
Standard phosphoramidites
0.1 M sodium borate buffer (pH 8.5)
For Ethanol Precipitation of Oligonucleotide
3 M sodium acetate buffer (pH 8.5)
Ice-cold absolute ethanol
For Oligonucleotide Purification by HPLC
HPLC
Vacuum centrifuge
POROS OligoR3 separation medium (PerSeptive Biosystems, Inc., 4.6 × 50 mm column). This separation medium is recommended to obtain optimal purification results.
100 mM Triethylammoniumacetate (pH 6.9)
Acetonitrile
Do not use Reversed Phase (RP) separation media because labeled oligonucleotides tend to stick irreversibly to the RP-material, resulting in lower yields of labeled oligonucleotides.
For Quality Control of HPLC-Purified Oligonucleotides