{ "ProductData": { "ID": "3.5.5.2.1.1", "ProductType": "Others", "BrandName": "LightCycler^® Red 640-N-hydroxysuccinimide ester", "ProductNameAddition": "", "RegulatoryDisclaimer1": "For life science research only. Not for use in diagnostic procedures.", "DisclaimerGroup1": "LSR", "RegulatoryDisclaimer2": null, "DisclaimerGroup2": null, "RegulatoryDisclaimer3": null, "SampleType": [ "Nucleic acid preparations" ], "LicenseDisclaimers": [ { "Identifier": "70051125019", "LongText": "This product is sold for use by purchaser only. The sale or distribution of this product or any product derived from the use of this product to any third party is expressly excluded. Especially excluded is the commercial use or sale of oligonucleotides or other products labeled with this product.", "Order": "15" } ], "RelatedLinks": "", "Clone": "", "ControlTissue": [ "" ], "ISOtypes": "", "Species": [ "" ], "StainLocalization": [ "" ], "ProductNameGlobal": "LightCycler Red 640-N-hydroxysuccinimide ester" }, "ProductImageDetails": { "ImagePath": "https://pim-media.roche.com/Images/Article_12015161001_im_en.png", "ImageType": "Image main" }, "Product2Taxonomy": { "Product2TaxonomyReferences": [ { "StructureSystemIdentifier": "OWP_Product_Types", "StructureSystemName": "Product Types", "NodeID": "95-000-00", "StructureNodeStatus": "Active", "NodeName": "Auxiliaries" }, { "StructureSystemIdentifier": "OWP_Family", "StructureSystemName": "Product Families", "NodeID": "230", "StructureNodeStatus": "Active", "NodeName": "LightCycler" }, { "StructureSystemIdentifier": "Product_Solutions", "StructureSystemName": "Product Solutions", "NodeID": "100", "StructureNodeStatus": "Active", "NodeName": "Molecular" }, { "StructureSystemIdentifier": "OWP_Techniques", "StructureSystemName": "Techniques", "NodeID": "320-00", "StructureNodeStatus": "Active", "NodeName": "Labeling and Detection" }, { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeID": "06-0074", "StructureNodeStatus": "Active", "NodeName": "LightCycler Red" }, { "StructureSystemIdentifier": "Applications", "StructureSystemName": "Applications", "NodeID": "33-00-00", "StructureNodeStatus": "Active", "NodeName": "Probe Labeling" }, { "StructureSystemIdentifier": "Lab_Type", "StructureSystemName": "Lab Types", "NodeID": "170-00", "StructureNodeStatus": "Inactive", "NodeName": "" }, { "StructureSystemIdentifier": "Disease_Areas", "StructureSystemName": "Disease Areas", "NodeID": "99-00-00", "StructureNodeStatus": "Inactive", "NodeName": "" }, { "StructureSystemIdentifier": "Pathogens", "StructureSystemName": "Pathogens", "NodeID": "99-00-00", "StructureNodeStatus": "Inactive", "NodeName": "" }, { "StructureSystemIdentifier": "Health_Topics", "StructureSystemName": "Health Topics", "NodeID": "99-00-00", "StructureNodeStatus": "Inactive", "NodeName": "" } ] }, "Product2Materials": { "P2MaterialReferences": [ { "MaterialNum": "12015161001", "MaterialDescription": "LC RED 640-NHS ester", "RegisteredProductName": "LightCycler Red 640-N-hydroxysuccinimide ester", "GTIN": "04038377016467", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "1 vial", "PackSizeDescPIM360": "for 5 x 50 nmol oligonucleotides", "MaterialAnnotation": "", "ReadyForUse": "", "OrderInformation": "" } ] }, "Product2Products": { "Product2ProductReference": [ ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Name": "Storage Conditions (Product)", "Value": "Store the product at −15 to −25°C.", "Language": "en", "Country": "XG", "Code": "Storage Conditions (Product)" }, { "Name": "Product Description", "Value": "Hydroxysuccinimide ester for labeling the 5' amino terminus of oligonucleotides with LightCycler^® Red 640.", "Language": "en", "Country": "XG", "Code": "Product Description" }, { "Name": "Applications", "Value": "The LightCycler^® Red 640-N-hydroxysuccinimide ester labels 5'-amino oligonucleotides that are used in combination with 3'-fluorescein-labeled oligonucleotides (LightCycler^® Fluorescein CPG) when using HybProbe probes for detection.", "Language": "en", "Country": "XG", "Code": "Applications" }, { "Name": "Additional Equipment and Reagent Required", "Value": "

• DNA synthesizer
• Standard reagents for oligonucleotide synthesis (tetrazol, etc.)
• Absolute, amine-free dimethylformamide (DMF)
• 5'-Amino modifier
• 3'-Phosphate CPG support
• Standard phosphoramidites
• 0.1 M sodium borate buffer (pH 8.5)

• 3 M sodium acetate buffer (pH 8.5)
• Ice-cold absolute ethanol

• HPLC
• Vacuum centrifuge
• POROS OligoR3 separation medium (PerSeptive Biosystems, Inc., 4.6 × 50 mm column). This separation medium is recommended to obtain optimal purification results.
• 100 mM Triethylammoniumacetate (pH 6.9)
• Acetonitrile

• Photometer

", "Language": "en", "Country": "XG", "Code": "Additional Equipment and Reagent Required" }, { "Name": "Principle", "Value": "LightCycler^® Red 640 shows an excitation maximum at 625 nm and an emission maximum at 640 nm (in 2 mM Tris-buffer, pH 8.3). Fig. 3: LightCycler® Red 640 excitation and emission spectra.
5'-amino-substituted-3'-phosphorylated oligonucleotides react with the LightCycler^® Red 640-NHS ester in a sodium borate buffer/dimethylformamide (DMF) mixture at pH 8.5 - 9.0.", "Language": "en", "Country": "XG", "Code": "Principle" }, { "Name": "Content", "Value": "

Vial/Bottle	Label	Function/Description	Content
1	LightCycler® Red 640 NHS-ester for ≥250 nmol oligonuc.	Contains a sufficient amount of LightCycler® Red 640-NHS ester for labeling a minimum of 5 × 50 nmol oligonucleotides. The reagent is supplied as a blue solid.	1 vial

", "Language": "en", "Country": "XG", "Code": "Content" }, { "Name": "Product Purpose", "Value": "Hydroxysuccinimide ester for labeling the 5' amino terminus of oligonucleotides with LightCycler^® Red 640.", "Language": "en", "Country": "XG", "Code": "Product Purpose" } ] } } ] }