For life science research only. Not for use in diagnostic procedures. Others LightCycler Red 640-N-hydroxysuccinimide ester LSR LightCycler® Red 640-N-hydroxysuccinimide ester 15 70051125019 This product is sold for use by purchaser only. The sale or distribution of this product or any product derived from the use of this product to any third party is expressly excluded. Especially excluded is the commercial use or sale of oligonucleotides or other products labeled with this product. 3.5.5.2.1.1 12015161001 LC RED 640-NHS ester LightCycler Red 640-N-hydroxysuccinimide ester 04038377016467 Reagents, kits 1 vial for 5 x 50 nmol oligonucleotides Hydroxysuccinimide ester for labeling the 5' amino terminus of oligonucleotides with LightCycler® Red 640. en The LightCycler® Red 640-NHS ester is used for labeling the 5' end of the downstream probe when HybProbe probes are used as detection format with the respective LightCycler® System. In addition, the 3' end of the probe has to be phosphorylated to avoid elongation during PCR. en Spectral CharacteristicsLightCycler® Red 640 shows an excitation maximum at 625 nm and an emission maximum at 640 nm (in 2 mM Tris-buffer, pH 8.3). Fig. 3: LightCycler® Red 640 excitation and emission spectra.Labeling Principle5'-amino-substituted-3'-phosphorylated oligonucleotides react with the LightCycler® Red 640-NHS ester in a sodium borate buffer/dimethylformamide (DMF) mixture at pH 8.5 - 9.0. en