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"Product Description" }, { "Language": "en", "Value": "The LightCycler^® Red 640-N-hydroxysuccinimide ester labels 5'-amino oligonucleotides that are used in combination with 3'-fluorescein-labeled oligonucleotides (LightCycler^® Fluorescein CPG) when using HybProbe probes for detection.", "Country": "XG", "Code": "Applications", "Name": "Applications" }, { "Language": "en", "Value": "

• DNA synthesizer
• Standard reagents for oligonucleotide synthesis (tetrazol, etc.)
• Absolute, amine-free dimethylformamide (DMF)
• 5'-Amino modifier
• 3'-Phosphate CPG support
• Standard phosphoramidites
• 0.1 M sodium borate buffer (pH 8.5)

• 3 M sodium acetate buffer (pH 8.5)
• Ice-cold absolute ethanol

• HPLC
• Vacuum centrifuge
• POROS OligoR3 separation medium (PerSeptive Biosystems, Inc., 4.6 × 50 mm column). This separation medium is recommended to obtain optimal purification results.
• 100 mM Triethylammoniumacetate (pH 6.9)
• Acetonitrile

• Photometer

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5'-amino-substituted-3'-phosphorylated oligonucleotides react with the LightCycler^® Red 640-NHS ester in a sodium borate buffer/dimethylformamide (DMF) mixture at pH 8.5 - 9.0.", "Country": "XG", "Code": "Principle", "Name": "Principle" }, { "Language": "en", "Value": "

Vial/Bottle	Label	Function/Description	Content
1	LightCycler® Red 640 NHS-ester for ≥250 nmol oligonuc.	Contains a sufficient amount of LightCycler® Red 640-NHS ester for labeling a minimum of 5 × 50 nmol oligonucleotides. The reagent is supplied as a blue solid.	1 vial

", "Country": "XG", "Code": "Content", "Name": "Content" } ] } } ] }