{ "ProductData": { "RelatedLinks": "", "RegulatoryDisclaimer1": "For life science research only. Not for use in diagnostic procedures.", "RegulatoryDisclaimer2": "", "StainLocalization": [ "" ], "ProductType": "Others", "DisclaimerGroup2": null, "ProductNameGlobal": "LightCycler Red 640-N-hydroxysuccinimide ester", "DisclaimerGroup1": "LSR", "ControlTissue": [ "" ], "BrandName": "LightCycler® Red 640-N-hydroxysuccinimide ester", "LicenseDisclaimers": [ { "Order": "15", "Identifier": "70051125019", "LongText": "This product is sold for use by purchaser only. The sale or distribution of this product or any product derived from the use of this product to any third party is expressly excluded. Especially excluded is the commercial use or sale of oligonucleotides or other products labeled with this product." } ], "Isotypes": "", "RegulatoryDisclaimer3": "", "ID": "3.5.5.2.1.1", "ProductNameAddition": "For labeling a minimum of 5 × 50 nmol oligonucleotides", "Species": [ "" ], "Clone": "" }, "ProductImageDetails": { "ImagePath": "https://pim-media.roche.com/Images/Article_12015161001_im_en.png", "ImageType": "Image main" }, "Product2Taxomony": { "Product2TaxomonyReferences": [ { "StructureSystemIdentifier": "OWP_Product_Types", "StructureSystemName": "Product Types", "NodeName": "Auxiliaries", "NodeID": "95-000-00" }, { "StructureSystemIdentifier": "OWP_Family", "StructureSystemName": "Product Families", "NodeName": "LightCycler", "NodeID": "230" }, { "StructureSystemIdentifier": "Product_Solutions", "StructureSystemName": "Product Solutions", "NodeName": "Molecular", "NodeID": "100" }, { "StructureSystemIdentifier": "OWP_Techniques", "StructureSystemName": "Techniques", "NodeName": "Labeling and Detection", "NodeID": "320-00" }, { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeName": "LightCycler® Red", "NodeID": "06-0074" }, { "StructureSystemIdentifier": "Applications", "StructureSystemName": "Applications", "NodeName": "Probe Labeling", "NodeID": "33-00-00" }, { "StructureSystemIdentifier": "Lab_Type", "StructureSystemName": "Lab Types", "NodeName": "LifeScience", "NodeID": "170-00" }, { "StructureSystemIdentifier": "Disease_Areas", "StructureSystemName": "Disease Areas", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Pathogens", "StructureSystemName": "Pathogens", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Health_Topics", "StructureSystemName": "Health Topics", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" } ] }, "Product2Taxonomy": { "Product2TaxonomyReferences": [ { "StructureSystemIdentifier": "OWP_Product_Types", "StructureSystemName": "Product Types", "NodeName": "Auxiliaries", "NodeID": "95-000-00" }, { "StructureSystemIdentifier": "OWP_Family", "StructureSystemName": "Product Families", "NodeName": "LightCycler", "NodeID": "230" }, { "StructureSystemIdentifier": "Product_Solutions", "StructureSystemName": "Product Solutions", "NodeName": "Molecular", "NodeID": "100" }, { "StructureSystemIdentifier": "OWP_Techniques", "StructureSystemName": "Techniques", "NodeName": "Labeling and Detection", "NodeID": "320-00" }, { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeName": "LightCycler® Red", "NodeID": "06-0074" }, { "StructureSystemIdentifier": "Applications", "StructureSystemName": "Applications", "NodeName": "Probe Labeling", "NodeID": "33-00-00" }, { "StructureSystemIdentifier": "Lab_Type", "StructureSystemName": "Lab Types", "NodeName": "LifeScience", "NodeID": "170-00" }, { "StructureSystemIdentifier": "Disease_Areas", "StructureSystemName": "Disease Areas", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Pathogens", "StructureSystemName": "Pathogens", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Health_Topics", "StructureSystemName": "Health Topics", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" } ] }, "Product2Materials": { "P2MaterialReferences": [ { "MaterialNum": "12015161001", "MaterialDescription": "LC RED 640-NHS ester", "RegisteredProductName": "LightCycler Red 640-N-hydroxysuccinimide ester", "GTIN": "04038377016467", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "1 vial", "PackSizeDescPIM360": "for 5 x 50 nmol oligonucleotides", "MaterialAnnotation": "", "ReadyForUse": "", "OrderInformation": "" } ] }, "Product2Products": { "Product2ProductReference": [ { "ProductID": "INS_409", "BrandName": "LightCycler® 2.0 Instrument", "ProductNameAddition": "", "ReferenceType": "Instrument", "Classification": [ { "IdentifierofStructureSystem": "1", "NameofStructureSystem": "GPCH", "StructureNodeID": "416", "StructureGroupPath": "Life Science->RLS qPCR & NAP Systems->LightCycler 2.0", "StructureGroupName": "LightCycler 2.0" }, { "IdentifierofStructureSystem": "OWP_Family", "NameofStructureSystem": "Product Families", "StructureNodeID": "230", "StructureGroupPath": "LightCycler", "StructureGroupName": "LightCycler" }, { "IdentifierofStructureSystem": "OWP_Product_Types", "NameofStructureSystem": "Product Types", "StructureNodeID": "10-000-00", "StructureGroupPath": "Analyzer Instruments and Systems", "StructureGroupName": "Analyzer Instruments and Systems" }, { "IdentifierofStructureSystem": "Lab_Type", "NameofStructureSystem": "Lab Types", "StructureNodeID": "150-00", "StructureGroupPath": "Research Lab", "StructureGroupName": "Research Lab" }, { "IdentifierofStructureSystem": "Product_Solutions", "NameofStructureSystem": "Product Solutions", "StructureNodeID": "100", "StructureGroupPath": "Molecular", "StructureGroupName": "Molecular" }, { "IdentifierofStructureSystem": "OWP_Techniques", "NameofStructureSystem": "Techniques", "StructureNodeID": "210-00", "StructureGroupPath": "PCR/qPCR", "StructureGroupName": "PCR/qPCR" }, { "IdentifierofStructureSystem": "OWP_Techniques", "NameofStructureSystem": "Techniques", "StructureNodeID": "300-00", "StructureGroupPath": "RT-PCR/qRT-PCR", "StructureGroupName": "RT-PCR/qRT-PCR" }, { "IdentifierofStructureSystem": "Applications", "NameofStructureSystem": "Applications", "StructureNodeID": "99-00-00", "StructureGroupPath": "NOT APPLICABLE", "StructureGroupName": "NOT APPLICABLE" }, { "IdentifierofStructureSystem": "Pathogens", "NameofStructureSystem": "Pathogens", "StructureNodeID": "99-00-00", "StructureGroupPath": "NOT APPLICABLE", "StructureGroupName": "NOT APPLICABLE" }, { "IdentifierofStructureSystem": "Disease_Areas", "NameofStructureSystem": "Disease Areas", "StructureNodeID": "99-00-00", "StructureGroupPath": "NOT APPLICABLE", "StructureGroupName": "NOT APPLICABLE" }, { "IdentifierofStructureSystem": "Health_Topics", "NameofStructureSystem": "Health Topics", "StructureNodeID": "99-00-00", "StructureGroupPath": "NOT APPLICABLE", "StructureGroupName": "NOT APPLICABLE" }, { "IdentifierofStructureSystem": "Product_Solutions", "NameofStructureSystem": "Product Solutions", "StructureNodeID": "055", "StructureGroupPath": "Laboratory developed testing", "StructureGroupName": "Laboratory developed testing" } ] } ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Language": "en", "Value": "Store the product at −15 to −25°C.", "Country": "XG", "Code": "Storage Conditions (Product)", "Name": "Storage Conditions (Product)" }, { "Language": "en", "Value": "Hydroxysuccinimide ester for labeling the 5' amino terminus of oligonucleotides with LightCycler® Red 640.", "Country": "XG", "Code": "Product Description", "Name": "Product Description" }, { "Language": "en", "Value": "The LightCycler® Red 640-N-hydroxysuccinimide ester labels 5'-amino oligonucleotides that are used in combination with 3'-fluorescein-labeled oligonucleotides (LightCycler® Fluorescein CPG) when using HybProbe probes for detection.", "Country": "XG", "Code": "Applications", "Name": "Applications" }, { "Language": "en", "Value": "
For Oligonucleotide Labeling
  • DNA synthesizer
  • Standard reagents for oligonucleotide synthesis (tetrazol, etc.)
  • Absolute, amine-free dimethylformamide (DMF)
  • 5'-Amino modifier
  • 3'-Phosphate CPG support
  • Standard phosphoramidites
  • 0.1 M sodium borate buffer (pH 8.5)
For Ethanol Precipitation of Oligonucleotide
  • 3 M sodium acetate buffer (pH 8.5)
  • Ice-cold absolute ethanol
For Oligonucleotide Purification by HPLC
  • HPLC
  • Vacuum centrifuge
  • POROS OligoR3 separation medium (PerSeptive Biosystems, Inc., 4.6 × 50 mm column). This separation medium is recommended to obtain optimal purification results.
  • 100 mM Triethylammoniumacetate (pH 6.9)
  • Acetonitrile
Do not use Reversed Phase (RP) separation media because labeled oligonucleotides tend to stick irreversibly to the RP-material, resulting in lower yields of labeled oligonucleotides.
For Quality Control of HPLC-Purified Oligonucleotides
  • Photometer
", "Country": "XG", "Code": "Additional Equipment and Reagent Required", "Name": "Additional Equipment and Reagent Required" }, { "Language": "en", "Value": "
Spectral Characteristics
LightCycler® Red 640 shows an excitation maximum at 625 nm and an emission maximum at 640 nm (in 2 mM Tris-buffer, pH 8.3). Fig. 1: Emission spectra of fluorescent dyes.
Labeling Principle
5'-amino-substituted-3'-phosphorylated oligonucleotides react with the LightCycler® Red 640-NHS ester in a sodium borate buffer/dimethylformamide (DMF) mixture at pH 8.5 - 9.0.", "Country": "XG", "Code": "Principle", "Name": "Principle" }, { "Language": "en", "Value": "
Vial/Bottle Label Function/Description Content
1 LightCycler® Red 640 NHS-ester for ≥250 nmol oligonuc.
  • Contains a sufficient amount of LightCycler® Red 640-NHS ester for labeling a minimum of 5 × 50 nmol oligonucleotides.
  • The reagent is supplied as a blue solid.
1 vial
", "Country": "XG", "Code": "Content", "Name": "Content" } ] } } ] }

LightCycler® Red 640-N-hydroxysuccinimide ester

For labeling a minimum of 5 × 50 nmol oligonucleotides

LightCycler<sup><sup>®</sup></sup> Red 640-N-hydroxysuccinimide ester

Ordering Information

Overview

Technical Documents

Access Material Data Sheets, Certificates of Analysis, and other product documentation.

After clicking above, you will be redirected to eLabDoc, where you can choose your local country.

Instruments

...
    ...
    error errorMessage
    Sorry, we couldn't find the content you are looking for
    Please try again later