For life science research only. Not for use in diagnostic procedures. Others LightCycler RNA Amplification Kit SYBR Green I LSR LightCycler® RNA Amplification Kit SYBR Green I 67 1280837236338 SYBR Green I is manufactured by Molecular Probes, Inc., and is provided under license from Molecular Probes, Inc., for direct research use for PCR, where the dye is present during the PCR. 3.5.5.1.2.1 12015137001 LC RNA Amplification Kit, SYBR Green I LightCycler RNA Amplification Kit SYBR Green I 04038377016443 Reagents, kits 1 kit 96 reactions of 20 μl final volume each false Generation of PCR products can be detected by measurement of the SYBR Green I fluorescence signal. SYBR Green I dye (already included in the Reaction Mix of the LightCycler® RNA Amplification Kit SYBR Green I) will emit a fluorescence signal (530 nm) only when intercalated into the DNA double helix. Therefore during PCR, the increase in SYBR Green I fluorescence is directly proportional to the amount of double-stranded DNA generated.Specificity and sensitivity of amplification reactions detected with SYBR Green I dye is greatly enhanced by combining amplification with a Melting Curve analysis. In Melting Curve analysis, the reaction mixture is slowly heated to 95°C, which causes melting of double-stranded DNA and a corresponding decrease of SYBR Green I fluorescence. The instrument continuously monitors this fluorescence decrease and displays it as melting peaks. Each melting peak represents the characteristic melting temperature of a particular DNA product (where the DNA is 50% double-stranded and 50% single-stranded). If PCR generated only one amplicon, Melting Curve analysis will show only one melting peak. If primer-dimers or other nonspecific products are present, they will be shown as additional melting peaks.How this Product WorksThe LightCycler® RNA Amplification Kit SYBR Green I is designed specifically for the SYBR Green I detection format using the LightCycler® Carousel-Based System. It is used to perform one-step RT-PCR in 20 μl glass capillaries. Amplification and online monitoring of the template RNA is achieved by a combined procedure on the LightCycler® Carousel-Based System Instruments. The results are interpreted directly after completing the PCR. The amplicon is detected by measurement of the SYBR Green I fluorescence signal.The LightCycler® RNA Amplification Kit SYBR Green I provides convenience, high performance, reproducibility, and minimizes contamination risk. All you need to supply is template RNA, PCR primers, and additional MgCl2 (if necessary).In principle, the LightCycler® RNA Amplification Kit SYBR Green I can be used for the amplification and detection of every RNA target. However, you would need to optimize each amplification protocol to the reaction conditions of the LightCycler® Carousel-Based System.The amplicon size should not exceed 1 kb in length. For optimal results, select a product length of 700 bp or less.The performance of the kit described in this Instructions for Use is guaranteed only when it is used with the LightCycler® Carousel-Based System. en The LightCycler® RNA Amplification Kit SYBR Green I is designed for use in life science research. The kit provides reagents, including RT-PCR enzyme mix, reaction mix, MgCl2, Resolution Solution, and Water, PCR Grade for one-step RT-PCR in glass capillaries using the LightCycler® Carousel-Based System and SYBR Green I as detection format.In addition, the kit can be used with Uracil-DNA Glycosylase, heat labile to prevent carryover contamination during PCR. en Easy-to-use Reaction Mix for One-Step RT-PCR, using SYBR Green I with the LightCycler® Carousel-Based System. en