For life science research only. Not for use in diagnostic procedures. Others LightCycler RNA Master SYBR Green I LSR LightCycler® RNA Master SYBR Green I 67 1280837236338 SYBR Green I is manufactured by Molecular Probes, Inc., and is provided under license from Molecular Probes, Inc., for direct research use for PCR, where the dye is present during the PCR. 3.5.5.1.2.5 03064760001 LC RNA Master SYBR Green I LightCycler RNA Master SYBR Green I 04038377017310 Reagents, kits 1 kit 96 reactions of 20 μl final volume each false LightCycler® RNA Master SYBR Green I is designed for use in research. When combined withthe LightCycler® Carousel-Based System, this kit uses a hot start RT-PCR protocol to provide very sensitive detection and quantification of defined RNA sequences (if suitable RT-PCR primers are supplied). The kit is especially suitable for difficult RNA populations, as the elevated incubation temperature during the reverse transcription step will help to overcome secondary structures. The hot start feature will minimize mispriming during the initial phase of the reaction and therefore overall sensitivity of RT-PCR is increased.In addition, the kit can be used with Uracil-DNA Glycosylase, heat-labile to prevent carryover contamination during PCR. en Easy-to-use SYBR Green I reaction mix for one-step RT-PCR, using the LightCycler® Carousel-Based System. en