Procedure | Time [min] |
---|---|
RT-PCR Setup | 15 |
Reverse Transcription | 20 |
LightCycler® Carousel-Based System PCR run | 25 |
Total Assay Time | 60 |
Vial/Bottle | Cap | Label | Function / Description | Content |
---|---|---|---|---|
1 | red | LightCycler® RNA Master SYBR Green I, LC RNA Master SYBR Green l, 2.7x conc. | Contains Tth DNA Polymerase, reaction buffer, dNTP mix (with dUTP instead of dTTP), and SYBR Green I. | 3 vials, 250 μl each |
2 | colorless | LightCycler® RNA Master SYBR Green I, Mn(OAc)2 stock solution, 50 mM | To adjust Mn(OAc)2 concentration in the reaction mix. | 1 vial, 1 ml |
3 | colorless | LightCycler® RNA Master SYBR Green I, Water, PCR Grade | To adjust the final reaction volume. | 2 vials, 1 ml each |
Easy-to-use reaction mix for one-step RT-PCR using the LightCycler® Carousel-Based System.