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Target material	Sample material
Genomic DNA	200, 500 or 1,000 μL whole blood up to 1 x 106 of cultured cells up to 5 mg of fresh-frozen tissue up to 6 mm3 formalin-fixed, paraffin-embedded tissue (FFPET)
Bacterial, fungal or viral NA	200, 500 or 1000 μL of plasma, serum, whole blood, bronchoalveolar lavage (BAL), nasopharyngeal/nasal swabs, stool, and urine.
Human cell-free NA	2,000 or 4,000 μL of plasma

The isolated and purified nucleic acids meet the quality standards required for highly sensitive quantitative PCR/RT-PCR analysis and next-generation sequencing.", "Country": "XG", "Code": "Applications", "Name": "Applications" }, { "Language": "en", "Value": "The MagNA Pure 24 System is an automated nucleic acid purification system consisting of the MagNA Pure 24 instrument, software, and consumables and reagents. The MagNA Pure 24 System is intended for use by professional users for the purification of nucleic acids from biological samples for in vitro diagnostic purposes.
The MagNA Pure 24 Total NA Isolation Kit is for use with the MagNA Pure 24 System.", "Country": "XG", "Code": "Intended Use", "Name": "Intended Use" }, { "Language": "en", "Value": "Warnings and precautions

• All human sourced material and all resulting waste should be handled as if infectious, using good laboratory procedures as outlined in Biosafety in Microbiological and Biomedical Laboratories and in the CLSI Document M29-A4^{1) 2)}.
• Only personnel proficient in handling infectious materials and the use of the MagNA Pure 24 System should perform the procedures described in this Instructions for Use.
• As the sensitivity and titer of potential pathogens in the sample material can vary, the operator must optimize pathogen inactivation and follow the appropriate measures according to local safety regulations.
• Closely follow procedures and guidelines provided to ensure that the nucleic acid isolation and purification is performed correctly. Any deviation from the procedures and guidelines may affect optimal purification performance.
• Use only reagents provided in this kit and buffers recommended in the Instructions for Use. Substitutions may introduce RNases.
• Use only supplied or specified required consumables to ensure optimal nucleic acid isolation and purification performance.

Reagent handling

• Several buffers in the MagNA Pure 24 Total NA Isolation Kit contain dangerous or hazardous compounds. Do not allow reagents to touch the skin, eyes, or mucous membranes. If contact does occur, wash the affected area immediately with large amounts of water. If the reagents are spilled, dilute the spill with water before wiping it up.
• Do not allow reagents containing guanidine thiocyanate (Lysis Buffer) to contact sodium hypochlorite (bleach) solution or acids. These mixtures can produce a highly toxic gas. This precaution is particularly important to be aware of when cleaning the processing station adapter, the liquid waste insert, tip waste container, and the reagent tip park. For more details regarding the cleaning maintenance, refer to the MagNA Pure 24 User Assistance.
• Before use, visually inspect the reagent cassettes to ensure that there are no signs of leakage. If there is any evidence of leakage, do not use that material for nucleic acid isolation and purification.
• Avoid microbial and nuclease contamination of reagents once they are opened.
• Immediately after use, cap all reagent bottles with their dedicated caps for reuse on the same instrument and store them according to their Instructions for Use.

Good laboratory practice

• Wear protective disposable gloves, laboratory coats, and eye protection when handling samples and reagents. Gloves must be changed between handling samples and reagents to prevent contamination.
• False positive results may occur if carryover of samples is not adequately controlled during sample handling and processing.
• Do not eat, drink, or smoke in the laboratory work area.
• Do not pipette by mouth.
• Wash hands thoroughly after handling samples and reagents, and after removing gloves.
• RNase-contaminated reagents and reaction vessels will degrade template RNA. Follow these guidelines to minimize the risk of contamination:
• Avoid touching surfaces or materials that could cause RNase carryover.
• Discard pipette tips in sealed containers to prevent airborne contamination.
• Clean, disinfect, and decontaminate work areas and instruments, including pipettes, with appropriate commercially available reagents.
• Use a work area specifically designated for RNA work. If possible, use reaction vessels and pipetters dedicated only for work with template RNA.
• If spillage occurs on the instrument, follow the cleaning instructions in the MagNA Pure 24 User Assistance.

Waste handling

• Safety Data Sheets (SDS) are available online at www.dialog.roche.com, or upon request from the local Roche office.

• Discard all materials that have come in contact with samples and reagents in accordance with country, state, and local regulations.
• Wear protective disposable gloves, laboratory coats, and eye protection when discarding samples and kit reagents.
• To discard the reagents from the containers, follow the procedure below:

1. Pierce the foil in the corner of one reagent container in the reagent cassette with a rigid plastic consumable, such as a serological pipette.
2. Fold back the foil and discard the liquid into a designated waste container.
3. Repeat steps 1 and 2 until all containers are empty.

", "Country": "XG", "Code": "Safety Information", "Name": "Safety Information" }, { "Language": "en", "Value": "The nucleic acid isolation procedure is based on the proven MagNA Pure Magnetic Glass Particle (MGP) Technology.
The key steps of this isolation procedure are:

1. Sample material is lysed, nucleic acids are released, and nucleases are denatured.
2. Nucleic acids bind to the silica surface of the added MGP due to the chaotropic salt conditions and the high ionic strength of the lysis/binding buffer.
3. MGP with bound nucleic acids are magnetically separated from the residual lysed sample.
4. Unbound substances, such as proteins, cell debris, and PCR inhibitors are removed by several washing steps.
5. Purified nucleic acids are eluted from the MGP.

", "Country": "XG", "Code": "Principle", "Name": "Principle" }, { "Language": "en", "Value": "Each kit is comprised of 3 sealed reagent cassettes and 12 magnetic glass particle (MGP) tubes. All kit components are ready-to-use.

One reagent cassette can be used for up to 6 individual runs on the same instrument within 28 days. For storage after first use, seal the reagent cassettes using the MagNA Pure Sealing Foil.

MGP tubes are for single use only.", "Country": "XG", "Code": "Content", "Name": "Content" }, { "Language": "en", "Value": "

Number of isolations	Sample material
3 x 32 isolations	Small volume: Up to 200 μL plasma, serum, whole blood, bronchoalveolar lavage (BAL), nasopharyngeal/nasal swabs, stool, urine, up to 5 x 105 cultured cells, and up to 5 mg of fresh-frozen tissue.
3 x 24 isolations	Large volume: 500 μL or 1000 μL of plasma, serum, whole blood, bronchoalveolar lavage (BAL), nasopharyngeal/nasal swabs, stool, urine, and up to 1 x 106 cultured cells. Up to 6 mm3 formalin-fixed, paraffin-embeded tissue, corresponding to 6 FFPET sections of 4 or 5 μm.
3 x 24 isolations	Extra-large volume: 2,000 μL or 4,000 μL of plasma. To process extra-large sample volumes, additional reagents are required.

", "Country": "XG", "Code": "Properties", "Name": "Properties" }, { "Language": "en", "Value": "Universal reagent kit in pre-filled and ready-to-use cassettes for isolating nucleic acids using the MagNA Pure 24 Instrument.", "Country": "XG", "Code": "Product Characteristics", "Name": "Product Characteristics" }, { "Language": "en", "Value": "The MagNA Pure 24 Total Nucleic Acid Kit eliminates unnecessary complexity by enabling efficient extraction of nucleic acids from 10 human sample types. Rely on this simplified reagent concept and choose one of the pre-optimized and pre-programmed protocols to extract up to 24 samples in just over an hour, plus:

• Mix batch sample types and primary tubes within one protocol
• Run sample volume inputs ranging from 200 µL to 4 mL
• Reuse reagent cassettes for later use by sealing them
• Let the system identify and track reagent usage with on-board barcode scanning
• Expand your applications with a multitude of compatible external lysis buffers
• Extract 8 samples in less than 30 minutes with the fast protocol

MagNA Pure 24 Total NA Isolation Kit contains prefilled and ready-to-use reagent cassettes, that can be easily loaded into their stations, and single use magnetic glass particles (MGPs) vials, that can be easily loaded into the reagent racks. Additional MGPs and external lysis buffers can be ordered separately to enhance your extraction workflow. A variety of targets from a wide range of sample types can be extracted efficiently on the MagNA Pure 24 System using one kit and one protocol in the same run. The different protocols are optimized for the following human sample materials:

• Whole Blood
• Plasma
• Serum
• Bronchoalveolar lavage
• Swab
• Urine
• Stool
• Fresh/Frozen Tissue

", "Country": "XG", "Code": "Product Description", "Name": "Product Description" } ] } } ] }