For life science research only. Not for use in diagnostic procedures. Others MagNA Pure 96 Cellular RNA Large Volume Kit LSR MagNA Pure 96 Cellular RNA Large Volume Kit 3.8.2.3.2.6 05467535001 MagNA Pure 96 Cellular RNA LV Kit MagNA Pure 96 Cellular RNA Large Volume Kit 04038377026312 Reagents, kits 1 kit 3 sets3 x 96 isolations false The MagNA Pure 96 Cellular RNA Large Volume Kit is used together with the MagNA Pure 96 Instrument to purify high-quality, intact RNA from up to 288 samples. Isolated RNA can be eluted in 50, 100, or 200 μl (depending on the Elution Volume selected in the MagNA Pure 96 Software). Purified RNA meets the quality standards required for sensitive and quantitative RT-PCR using the LightCycler® Instruments.The RNA isolation procedure is based on the proven MagNA Pure Magnetic Glass Particle Technology. The principle steps of a MagNA Pure 96 RNA isolation procedure are:The sample material is lysed, nucleic acids are released, and nucleases are denatured.The nucleic acids bind to the silica surface of the added MGP due to the chaotropic salt conditions and the high ionic strength of the lysis/binding buffer.Genomic DNA is removed by incubation with DNase.MGP with bound RNA are magnetically separated from the residual lysed sample.Unbound substances, such as proteins, cell debris, and PCR inhibitors are removed by several washing steps.Purified RNA is eluted from the MGP. en Purification of total RNA from:Up to 1 × 106 cultured cells400 μl whole blood or a half PAXgene tube800 μl whole blood or a whole PAXgene tube with a batch of 48Up to 25 mg fresh-frozen tissue5 mg tissue stabilized in RNAlater1 – 10 μm sections from formalin-fixed paraffin-embedded tissueFor detailed package insert information, as well as Roche and customer provided applications, please visit us at: www.magnapure.com and www.magnapure96.roche.com. Purification protocol will also be available at the above listed websites.  en The MagNA Pure 96 Cellular RNA Large Volume Kit is specifically designed to isolate highly purified cellular RNA from cultured cells, or whole blood stabilized with the RNA/DNA Stabilization Reagent for Blood/Bone Marrow, or whole blood stored in PAXgene Blood RNA tubes, or from fresh-frozen tissue, or tissue stabilized in RNAlater, or from 1 - 10 μm sections from formalin-fixed, paraffin-embedded tissue, using the MagNA Pure 96 Instrument.Purified RNA can be used for RT-PCR with LightCycler® Instruments or standard thermal block cyclers.The kit is designed to process up to:3 × 96 isolations from up to 1 × 106 cultured cells.3 × 96 isolations of 400 μl whole blood or a half PAXgene tube.3 × 48 isolations of 800 μl whole blood or a whole PAXgene tube.3 × 48 isolations from up to 25 mg fresh-frozen tissue.3 × 96 isolations from up to 20 mg fresh-frozen tissue or tissue stabilized in RNAlater.3 × 96 isolations from 1-10 μm sections, depending on the pretreatment from formalin-fixed or paraffin-embedded tissue. en