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• MagNA Pure 96 System Fluid (Internal) or (External)*
• MagNA Pure 96 Sealing Foil*
• MagNA Pure Tip 1000μL*
• MagNA Pure 96 Processing Cartridge*
• MagNA Pure 96 Output Plate*
• Standard laboratory equipment
• Pipettes and nuclease-free, aerosol-preventive tips to predispense samples into the MagNA Pure 96 Processing Cartridge: Standard length tips, or optional, extra-long tips of 10 cm length.
• Centrifuge for tubes (e.g., for PAXgene Blood RNA tubes)
• Phosphate buffered saline (PBS)
• Optional: RNA/DNA Stabilization Reagent for Blood/Bone Marrow*
• Optional, PAXgene Blood RNA tubes (Cat. No. 762165, available from PreAnalytix)
• Optional, new lids for PAXgene Blood RNA tubes (e.g., flexible Vacucap Closures (16 mm), available from VWR)
• Optional, MagNA Pure LC RNA Tissue Lysis Buffer/Refill* (for fresh-frozen tissue applications)
• Optional, MagNA Lyser Instrument* or similar device for homogenization of fresh-frozen tissue
• Optional, MagNA Lyser Green Beads*
• Optional, MagNA Pure DNA Tissue Lysis Buffer* for FFPE tissue applications
• Optional, MagNA Pure FFPET Buffer Set* for FFPE tissue applications
• Optional, Proteinase K (solution)* for FFPE tissue applications
• Optional, Xylol, for FFPE tissue applications
• Optional, Ethanol, for FFPE tissue applications
• Vortex mixer or multiple vortex mixer
• RNAlater

", "Country": "XG", "Code": "Additional Equipment and Reagent Required", "Name": "Additional Equipment and Reagent Required" }, { "Language": "en", "Value": "

MagNA Pure 96 Instrument setup	5 to 10 minutes
Instrument run time	75 to 90 minutes

", "Country": "XG", "Code": "Assay Time", "Name": "Assay Time" }, { "Language": "en", "Value": "The MagNA Pure 96 Cellular RNA Large Volume Kit is used together with the MagNA Pure 96 Instrument to purify high-quality, intact RNA from up to 288 samples. Isolated RNA can be eluted in 50, 100, or 200 μl (depending on the Elution Volume selected in the MagNA Pure 96 Software). Purified RNA meets the quality standards required for sensitive and quantitative RT-PCR using the LightCycler^® Instruments.

The RNA isolation procedure is based on the proven MagNA Pure Magnetic Glass Particle Technology. The principle steps of a MagNA Pure 96 RNA isolation procedure are:

1. The sample material is lysed, nucleic acids are released, and nucleases are denatured.
2. The nucleic acids bind to the silica surface of the added MGP due to the chaotropic salt conditions and the high ionic strength of the lysis/binding buffer.
3. Genomic DNA is removed by incubation with DNase.
4. MGP with bound RNA are magnetically separated from the residual lysed sample.
5. Unbound substances, such as proteins, cell debris, and PCR inhibitors are removed by several washing steps.
6. Purified RNA is eluted from the MGP.

", "Country": "XG", "Code": "Principle", "Name": "Principle" }, { "Language": "en", "Value": "

Component	Label	Function / Description	Content
Tray 1	Reagent Tray 1		3 trays
Container 1	Wash Buffer I	for removing impurities	1 container
Container 2	Wash Buffer II	for removing impurities	1 container
Tray 2	Reagent Tray 2		3 trays
Container 1	Lysis/Binding Buffer	for cell lysis and binding of RNA	1 container
Container 2	Proteinase K	for digestion of proteins	1 container
Container 3	Proteinase K Incubation Buffer	for digestion of proteins	1 container
Container 4	DNase Incubation Buffer	for digestion of DNA	1 container
Container 5	Elution Buffer	for elution of RNA	1 container
Bottle 1	Magnetic Glass Particles	MGP suspension (brownish to black solution) for binding RNA	6 bottles
Bottle 2	DNase	lyophilizate for digestion of DNA	6 glass vials
Bottle 3	DNase Incubation Buffer	for reconstituion of DNase	3 bottles

", "Country": "XG", "Code": "Content", "Name": "Content" }, { "Language": "en", "Value": "Purification of total RNA from:

• Up to 1 × 10⁶ cultured cells
• 400 μl whole blood or a half PAXgene tube
• 800 μl whole blood or a whole PAXgene tube with a batch of 48
• Up to 25 mg fresh-frozen tissue
• 5 mg tissue stabilized in RNAlater
• 1 – 10 μm sections from formalin-fixed paraffin-embedded tissue

For detailed package insert information, as well as Roche and customer provided applications, please visit us at: www.magnapure.com and www.magnapure96.roche.com. Purification protocol will also be available at the above listed websites.


 ", "Country": "XG", "Code": "Product Description", "Name": "Product Description" }, { "Language": "en", "Value": "The MagNA Pure 96 Cellular RNA Large Volume Kit is specifically designed to isolate highly purified cellular RNA from cultured cells, or whole blood stabilized with the RNA/DNA Stabilization Reagent for Blood/Bone Marrow, or whole blood stored in PAXgene Blood RNA tubes, or from fresh-frozen tissue, or tissue stabilized in RNAlater, or from 1 - 10 μm sections from formalin-fixed, paraffin-embedded tissue, using the MagNA Pure 96 Instrument.
Purified RNA can be used for RT-PCR with LightCycler^® Instruments or standard thermal block cyclers.

• 3 × 96 isolations from up to 1 × 10⁶ cultured cells.
• 3 × 96 isolations of 400 μl whole blood or a half PAXgene tube.
• 3 × 48 isolations of 800 μl whole blood or a whole PAXgene tube.
• 3 × 48 isolations from up to 25 mg fresh-frozen tissue.
• 3 × 96 isolations from up to 20 mg fresh-frozen tissue or tissue stabilized in RNAlater.
• 3 × 96 isolations from 1-10 μm sections, depending on the pretreatment from formalin-fixed or paraffin-embedded tissue.

", "Country": "XG", "Code": "Applications", "Name": "Applications" }, { "Language": "en", "Value": "Prefilled reagents for the isolation of cellular RNA from up to 1 × 10⁶ cultured cells, or up to 800 μl whole blood, or whole blood stabilized in PAXgene Blood RNA tubes, or up to 25 mg fresh- frozen tissue, or tissue stabilized in RNAlater, or from 1 - 10 μm sections from formalin-fixed, paraffin-embedded tissue, using the MagNA Pure 96 Instrument.", "Country": "XG", "Code": "Product Characteristics", "Name": "Product Characteristics" } ] } } ] }