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< 6 M Guanidine hydrochloride, < 50% EtOH, < 30 mM Tris HCl
for removing impurities
Container 2 - Wash Buffer I
80 ml
< 6 M Guanidine hydrochloride, < 50% EtOH, < 30 mM Tris HCl
for removing impurities
Container 3 - Lysis/Binding Buffer
80 ml
< 6 M Guanidine SCN, < 30% Triton X-100, < 60 mM Tris HCl
for cell/virus lysis and binding of nucleic acids
Reagent Tray 2 (3 trays per kit) Container 1 - empty Container 2 - Proteinase K
15 ml
2% Proteinase K, 50% Glycerol
for digestion of proteins
Container 3 - Elution Buffer
80 ml
< 60 mM Tris-HCl buffer
for elution of nucleic acids
Container 4 - Wash Buffer III
160 ml
< 20 mM Na-acetate buffer
for removing impurities
Bottle 1 - Magnetic Glass Particles
6 bottles, 18 ml each
MGP suspension containing isopropanol
for binding nucleic acids
The MagNA Pure 96 System Fluid (Internal/External) serves as Wash Buffer II for this kit. All kit components are ready-to-use.",
"Country": "XG",
"Code": "Content",
"Name": "Content"
},
{
"Language": "en",
"Value": "The nucleic acid isolation procedure is based on the MagNA Pure Magnetic Glass Particle (MGP) Technology. The key steps of a MagNA Pure 96 NA isolation procedure are:
Sample material is lysed, nucleic acids are released, and nucleases are denatured.
Nucleic acids bind to the silica surface of the added MGPs due to the chaotropic salt conditions and the high ionic strength of the lysis/binding buffer.
MGPs with bound nucleic acids are magnetically separated from the residual lysed sample.
Unbound substances, such as proteins, cell debris, and PCR inhibitors are removed by several washing steps.
Purified nucleic acids are eluted from the MGPs.
",
"Country": "XG",
"Code": "Principle",
"Name": "Principle"
},
{
"Language": "en",
"Value": "The MagNA Pure 96 DNA and Viral NA Small Volume Kit is specifically designed to isolate:
Nucleic acid from up to 200 μl whole blood, plasma, or serum.
Bacterial, fungal, and viral nucleic acid from 200 μl sample material or lysate of human origin.
Nucleic acid from up to 5 × 105 cultured cells.
Nucleic acid from up to 5 mg fresh-frozen tissue, or from 1 - 10 μm sections from formalin-fixed, paraffin-embedded tissue.
The isolated nucleic acids meet the quality standards required for highly sensi- tive quantitative PCR/RT-PCR analysis and next-generation sequencing.",
"Country": "XG",
"Code": "Product Description",
"Name": "Product Description"
},
{
"Language": "en",
"Value": "Ready-to-use reagents in two prefilled sealed trays for purifying nucleic acids from up to 200 μL sample volumes using the MagNA Pure 96 Instrument.",
"Country": "XG",
"Code": "Product Characteristics",
"Name": "Product Characteristics"
}
]
}
}
]
}
MagNA Pure 96 DNA and Viral NA Small Volume Kit
Ready-to-use reagents in two prefilled sealed trays for purifying nucleic acids from up to 200 μL sample volumes using the MagNA Pure 96 Instrument.