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Container 1 - Wash Buffer I

• 160 ml
• < 6 M Guanidine hydrochloride, < 50% EtOH, < 30 mM Tris HCl
• for removing impurities

Container 2 - Wash Buffer I

• 80 ml
• < 6 M Guanidine hydrochloride, < 50% EtOH, < 30 mM Tris HCl
• for removing impurities

Container 3 - Lysis/Binding Buffer

• 80 ml
• < 6 M Guanidine SCN, < 30% Triton X-100, < 60 mM Tris HCl
• for cell/virus lysis and binding of nucleic acids

Reagent Tray 2 (3 trays per kit)
Container 1 - empty
Container 2 - Proteinase K

• 15 ml
• 2% Proteinase K, 50% Glycerol
• for digestion of proteins

Container 3 - Elution Buffer

• 80 ml
• < 60 mM Tris-HCl buffer
• for elution of nucleic acids

Container 4 - Wash Buffer III

• 160 ml
• < 20 mM Na-acetate buffer
• for removing impurities

Bottle 1 - Magnetic Glass Particles

• 6 bottles, 18 ml each
• MGP suspension containing isopropanol
• for binding nucleic acids

The MagNA Pure 96 System Fluid (Internal/External) serves as Wash Buffer II for this kit.
All kit components are ready-to-use.", "Country": "XG", "Code": "Content", "Name": "Content" }, { "Language": "en", "Value": "The nucleic acid isolation procedure is based on the MagNA Pure Magnetic Glass Particle (MGP) Technology.
The key steps of a MagNA Pure 96 NA isolation procedure are:

1. Sample material is lysed, nucleic acids are released, and nucleases are denatured.
2. Nucleic acids bind to the silica surface of the added MGPs due to the chaotropic salt conditions and the high ionic strength of the lysis/binding buffer.
3. MGPs with bound nucleic acids are magnetically separated from the residual lysed sample.
4. Unbound substances, such as proteins, cell debris, and PCR inhibitors are removed by several washing steps.
5. Purified nucleic acids are eluted from the MGPs.

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• Nucleic acid from up to 200 μl whole blood, plasma, or serum.
• Bacterial, fungal, and viral nucleic acid from 200 μl sample material or lysate of human origin.
• Nucleic acid from up to 5 × 10⁵ cultured cells.
• Nucleic acid from up to 5 mg fresh-frozen tissue, or from 1 - 10 μm sections from formalin-fixed, paraffin-embedded tissue.

The isolated nucleic acids meet the quality standards required for highly sensi- tive quantitative PCR/RT-PCR analysis and next-generation sequencing.", "Country": "XG", "Code": "Product Description", "Name": "Product Description" }, { "Language": "en", "Value": "Ready-to-use reagents in two prefilled sealed trays for purifying nucleic acids from up to 200 μL sample volumes using the MagNA Pure 96 Instrument.", "Country": "XG", "Code": "Product Characteristics", "Name": "Product Characteristics" } ] } } ] }