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5,000 reactions of 40 μl final volume each", "MaterialAnnotation": "", "ReadyForUse": "", "OrderInformation": "" } ] }, "Product2Products": { "Product2ProductReference": [ ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Name": "Storage Conditions (Product)", "Value": "Store the buffer at +2 to +8°C.", "Language": "en", "Country": "XG", "Code": "Storage Conditions (Product)" }, { "Name": "Additional Equipment and Reagent Required", "Value": "
  • Standard laboratory equipment
  • Pipettes and nuclease free, aerosol-preventative tips
  • Phosphate Buffered Saline (PBS) for washing the cells
  • Protector RNase Inhibitor:
  • Protector RNase Inhibitor 2,000 U; Cat. No. 03335399001
  • Protector RNase Inhibitor 10,000 U; Cat. No. 03335402001
", "Language": "en", "Country": "XG", "Code": "Additional Equipment and Reagent Required" }, { "Name": "Product Characteristics", "Value": "Easy-to-use reagent for cell lysis, prior to real-time RT-PCR applications.", "Language": "en", "Country": "XG", "Code": "Product Characteristics" }, { "Name": "Content", "Value": "
Vial / BottleFunction / DescriptionContent
1RealTime ready Cell Lysis Buffer200 ml easy-to-use Lysis Buffer
", "Language": "en", "Country": "XG", "Code": "Content" }, { "Name": "Sample Materials", "Value": "To obtain optimal results in downstream procedures, especially in real-time RT-PCR assays on the LightCycler® Instruments, do not process samples larger than this reagent is designed to handle.

The optimal amount of sample material is as follows:
  • For adherent cells only: Seed an appropriate number of adherent cells and grow them overnight.
  • For suspension cells only: Cells can be lysed directly in their cell culture media, or first pelleted and optionally resuspended in PBS before lysis.
  • The maximal cell number and the volume of RealTime ready Cell Lysis Buffer depends on the type of culture plate used, but one lysis reaction can be done on a range of 3 to 3 × 104 cells.

Tissue Culture Plate TypeNumber of Cells per Lysis Reaction
(or per well)
96-wellUp to 30,000(1)
384-wellUp to 5,000
1536-wellUp to 2,000
  1. Depending on the cell size, up to 105 cells can be used. Although, higher concentrations may result in loss of signal, as the maximal number of adherent cells is restricted by cell density.
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The quality of RNA isolated is suitable for relative quantification of mRNA by Transcriptor One-Step RT-PCR Kit, especially on the LightCycler® Instruments.", "Language": "en", "Country": "XG", "Code": "Background Information" }, { "Name": "Quality Control", "Value": "For lot-specific certificates of analysis, see section Contact and Support.", "Language": "en", "Country": "XG", "Code": "Quality Control" }, { "Name": "Principle", "Value": "RealTime ready Cell Lysis Buffer is an easy-to-use reagent for lysing cultured cells in 96-/ 384-/1536-well plates, prior to reverse transcription or qPCR. This product is designed specifically for gene expression assays using a LightCycler® Master Mix on the LightCycler® 96 System (96-well format), LightCycler® 480 System (96- or 384-well format), or the LightCycler® 1536 System (1536-well format).

The isolation of RNA from experimental samples is the first step in gene expression studies. RNA isolation can be a quite time-consuming and intensive process, especially when dealing with samples with small amounts of material/cells or when dealing with a high number of samples. The RealTime ready Cell Lysis Buffer is designed to quickly lyse cultured cells prior to reverse transcription, without purifying the RNA, thus ensuring minimal loss of RNA and simplification of the whole gene expression workflow.", "Language": "en", "Country": "XG", "Code": "Principle" }, { "Name": "Product Description", "Value": "RealTime ready Cell Lysis Buffer is an easy-to-use reagent for the lysis of cultured cells prior to reverse transcription.", "Language": "en", "Country": "XG", "Code": "Product Description" }, { "Name": "Assay Time", "Value": "Total Time for Cell Lysis: 5 min
Optional wash step adds 5 min to the lysis protocol.", "Language": "en", "Country": "XG", "Code": "Assay Time" }, { "Name": "Product Purpose", "Value": "RealTime ready Cell Lysis Buffer is an easy-to-use reagent for the lysis of cultured cells prior to reverse transcription.", "Language": "en", "Country": "XG", "Code": "Product Purpose" } ] } } ] }

RealTime ready Cell Lysis Buffer

Easy-to-use reagent for cell lysis, prior to real-time RT-PCR applications.

LSR For life science research only. Not for use in diagnostic procedures.
RealTime ready Cell Lysis Buffer

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