For life science research only. Not for use in diagnostic procedures. Others RealTime ready Cell Lysis Buffer LSR RealTime ready Cell Lysis Buffer 3.8.1.5.8.2 RealTime ready Cell Lysis Buffer 04038377029177 1 bottle RTr Cell Lysis Buffer 07248431001 200 ml5,000 reactions of 40 μl final volume each Reagents, kits en The RealTime ready Cell Lysis Buffer is designed for the lysis of cultured cells. Cell lysates can then be directly used for RT-qPCR or cDNA synthesis followed by a qPCR step. The quality of RNA isolated is suitable for relative quantification of mRNA by qPCR. The RealTime ready Cell Lysis Buffer is designed for rapid, high-performance gene expression studies using cultured cells in RT-qPCR or qPCR applications. The buffer uses an advanced one-step protocol for lysing 3 to 30,000 cells within 5 minutes at room temperature. The resulting lysate can be used directly for downstream real-time PCR applications, eliminating the time-consuming RNA purification step and ensuring minimal loss of RNA. With this large pack size and easy-to-use protocol, the RealTime ready Cell Lysis Buffer can be easily adapted to high throughput experiments while minimizing waste materials. In addition, this buffer easily integrates into automated cell-based gene expression workflows. en RealTime ready Cell Lysis Buffer is an easy-to-use reagent for the lysis of cultured cells prior to reverse transcription. The buffer is designed for 5,000 lysis reactions, with a final volume of 40 μl each, or for 50,000 lysis reactions, with a final volume of 4 μl each. en RealTime ready Cell Lysis Buffer is an easy-to-use reagent for lysing cultured cells in 96-/ 384-/1536-well plates, prior to reverse transcription or qPCR. This product is designed specifically for gene expression assays using a LightCycler® Master Mix on the LightCycler® 96 System (96-well format), LightCycler® 480 System (96- or 384-well format), or the LightCycler® 1536 System (1536-well format).The isolation of RNA from experimental samples is the first step in gene expression studies. RNA isolation can be a quite time-consuming and intensive process, especially when dealing with samples with small amounts of material/cells or when dealing with a high number of samples. The RealTime ready Cell Lysis Buffer is designed to quickly lyse cultured cells prior to reverse transcription, without purifying the RNA, thus ensuring minimal loss of RNA and simplification of the whole gene expression workflow. en RealTime ready Cell Lysis Buffer is an easy-to-use reagent for the lysis of cultured cells prior to reverse transcription.