For life science research only. Not for use in diagnostic procedures. Others RealTime ready Cell Lysis Buffer LSR RealTime ready Cell Lysis Buffer 3.8.1.5.8.2 07248431001 RTr Cell Lysis Buffer RealTime ready Cell Lysis Buffer 04038377029177 Reagents, kits 1 bottle 200 ml5,000 reactions of 40 μl final volume each RealTime ready Cell Lysis Buffer is designed for the lysis of cultured cells. Cell lysates can then be directly used for RT-qPCR or cDNA synthesis followed by a qPCR step on the LightCycler® Instruments. The quality of RNA isolated is suitable for relative quantification of mRNA by qPCR, especially on the LightCycler® Instruments.Cell lysates can be processed immediately for RT-qPCR or cDNA synthesis, or stored frozen at -15 to -25°C, or stored at +2 to +8°C for a maximum of 2 days. en RealTime ready Cell Lysis Buffer is an easy-to-use reagent for lysing cultured cells in 96-/ 384-/1536-well plates, prior to reverse transcription or qPCR. This product is designed specifically for gene expression assays using a LightCycler® Master Mix on the LightCycler® 96 System (96-well format), LightCycler® 480 System (96- or 384-well format), or the LightCycler® 1536 System (1536-well format).The isolation of RNA from experimental samples is the first step in gene expression studies. RNA isolation can be a quite time-consuming and intensive process, especially when dealing with samples with small amounts of material/cells or when dealing with a high number of samples. The RealTime ready Cell Lysis Buffer is designed to quickly lyse cultured cells prior to reverse transcription, without purifying the RNA, thus ensuring minimal loss of RNA and simplification of the whole gene expression workflow. en RealTime ready Cell Lysis Buffer is an easy-to-use reagent for the lysis of cultured cells prior to reverse transcription. en