For life science research only. Not for use in diagnostic procedures. Others RealTime ready Cell Lysis Kit LSR RealTime ready Cell Lysis Kit 3.8.1.5.8.1 05943523001 RealTime ready Cell Lysis Kit RealTime ready Cell Lysis Kit 04038377027197 Reagents, kits 1 kit 500 lysis reactions with a final reaction volume of 40 μL each 06366821001 RealTime ready Cell Lysis Kit, 50 rx RealTime ready Cell Lysis Kit 04038377027647 Reagents, kits 1 kit 50 lysis reactions with a final reaction volume of 40 μL each RealTime ready Cell Lysis Kit is designed for the lysis of cultured cells. Cell lysates can then be directly used for cDNA synthesis, using a reverse transcription kit for cDNA synthesis*, followed by an appropriate PCR reagent for use on the LightCycler® Instruments or standard thermal block cyclers. The quality of RNA isolated is suitable for relative quantification of mRNA by RT-PCR, especially on the LightCycler® Instruments.Cell lysates can be processed immediately for RT-PCR, or stored frozen at −15 to −25°C, or stored at +2 to +8°C for a maximum of 2 days. en How this Product WorksRealTime ready Cell Lysis Kit is an easy-to-use reagent for lysing cultured cells in 96- and 384-well plates, prior to reverse transcription. This product is designed specifically for gene expression assays using the Universal ProbeLibrary and a LightCycler® Master mix on the LightCycler® 480 System (96- or 384-well format), LightCycler® 96 Instrument or LightCycler® 1536 Instrument.All that is required are the cells, RT-PCR reagents, primers and Universal ProbeLibrary probe.Test PrincipleThe isolation of RNA from experimental samples is the first step in gene expression studies. RNA isolation can be a quite time-consuming and intensive process, especially when dealing with samples with small amounts of material/cells.The RealTime ready Cell Lysis Kit is designed to quickly lyse cultured cells, prior to reverse transcription, without purifying the RNA, thus ensuring minimal loss of RNA and simplification of the whole gene expression workflow.The basic steps of using the RealTime ready Cell Lysis Kit and subsequent RT-PCR are:1. Cultured cells, seeded in a 96-, or 384-well plate are washed with ice-cold PBS.2. Cells are lysed by the addition of the Cell Lysis Reagent and incubation at room temperature. RNA is released from the lysed cells and protected from degradation, due to the presence of Protector RNase Inhibitor in the Cell Lysis Reagent.3. Cell lysates are reverse transcribed into cDNA with Transcriptor Reverse Transcription. Genomic DNA from the cell lysate is optionally degraded by the addition of Thermolabile Nuclease (provided in the RealTime ready Cell Lysis Kit) to the reverse transcription reaction and a 10 min incubation at 29°C, prior to the reverse transcription step.4. cDNA is then amplified using e.g. LightCycler® 480 Probes Master, or RealTime ready DNA Probes Master and the Universal ProbeLibrary. en