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With its 50-fold concentrated mixture of Transcriptor and Taq DNA Polymerase, and a 5-fold reaction buffer containing dNTPs and MgCl₂, the formulation is ideal for the detection of viral RNA. The proprietary reaction buffer allows fast and convenient \"hot start\" RT-PCR without heat pre-activation of the Taq DNA Polymerase. The kit is optimized for HybProbe probes and hydrolysis probes, as well as for Universal ProbeLibrary (UPL) probes, and does not require optimization of MgCl₂ concentration.", "Language": "en", "Country": "XG", "Code": "Applications" }, { "Name": "Assay Time", "Value": "The RealTime ready RNA Virus Master can be used for fast RT-PCR protocols with run times of 25 - 50 min using a LightCycler^® 480 System or a LightCycler^® Carousel-Based System.", "Language": "en", "Country": "XG", "Code": "Assay Time" }, { "Name": "Product Description", "Value": "The RealTime ready RNA Virus Master is designed for fast, high sensitive and specific real-time one-step RT-PCR analysis of viral RNA.", "Language": "en", "Country": "XG", "Code": "Product Description" }, { "Name": "Content", "Value": "

Vial / Bottle	Cap	Label	Function	Catalog Number	Content
1	red	Enzyme Blend, 50x conc.	Contains Transcriptor RT, Taq DNA Polymerase and proprietary additives. No activation at +95°C is necessary.	05 619 416 001	1 vial, 40 μl
05 992 877 001	10 vials, 40 μl each
2	colorless	Reaction Buffer, 5x conc.	Contains RT-PCR Reaction Buffer, dATP, dCTP, dGTP and dUTP, MgCl2, and proprietary additives. Keep vial 2 away from light!	05 619 416 001	1 vial, 400 μl
05 992 877 001	10 vials, 400 μl each
3	colorless	Water, PCR grade	To adjust the final reaction volume.	05 619 416 001	1 vial, 1 ml
05 992 877 001	10 vials,1 ml each

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