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With its 50-fold concentrated mixture of Transcriptor and Taq DNA Polymerase, and a 5-fold reaction buffer containing dNTPs and MgCl2, the formulation is ideal for the detection of viral RNA. The proprietary reaction buffer allows fast and convenient \"hot start\" RT-PCR without heat pre-activation of the Taq DNA Polymerase. The kit is optimized for HybProbe probes and hydrolysis probes, as well as for Universal ProbeLibrary (UPL) probes, and does not require optimization of MgCl2 concentration.", "Country": "XG", "Code": "Applications", "Name": "Applications" }, { "Language": "en", "Value": "
Typical Run Time
The RealTime ready RNA Virus Master can be used for fast RT-PCR protocols with run times of 25 - 50 min using a LightCycler® 480 System or a LightCycler® Carousel-Based System.", "Country": "XG", "Code": "Assay Time", "Name": "Assay Time" }, { "Language": "en", "Value": "The RealTime ready RNA Virus Master is designed for fast, high sensitive and specific real-time one-step RT-PCR analysis of viral RNA.", "Country": "XG", "Code": "Product Description", "Name": "Product Description" }, { "Language": "en", "Value": "
Vial / BottleCapLabelFunctionCatalog NumberContent
1redEnzyme Blend, 50x conc.
 
Contains Transcriptor RT, Taq DNA Polymerase and proprietary additives. No activation at +95°C is necessary.
 
05 619 416 0011 vial, 40 μl 
05 992 877 00110 vials, 40 μl each
2colorlessReaction Buffer, 5x conc.Contains RT-PCR Reaction Buffer, dATP, dCTP, dGTP and dUTP, MgCl2, and proprietary additives.
Keep vial 2 away from light!
05 619 416 0011 vial, 400 μl 
05 992 877 00110 vials, 400 μl each
3colorlessWater, PCR gradeTo adjust the final reaction volume.05 619 416 0011 vial, 1 ml
05 992 877 00110 vials,1 ml each
", "Country": "XG", "Code": "Content", "Name": "Content" } ] } } ] }

RealTime ready RNA Virus Master

Easy-to-use reaction mix for one-step RT-PCR using with the LightCycler® 480 System or the LightCycler® Carousel-Based System

RealTime ready RNA Virus Master

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