For life science research only. Not for use in diagnostic procedures. Others RNA Process Control Kit LSR LSR RNA Process Control Kit LSR 3.5.9.5.2.80 07958293001 RNA Process Control Kit LSR RNA Process Control Kit LSR 07613336103128 Reagents, kits 1 kit 6 × 96 control reactions and 600 RT-qPCR reactions of 20 µl final volume each false The RNA Process Control Kit LSR contains a nuclease-resistant RNA that can be added at various steps during the nucleic acid purification of viral RNA detection workflows. Successful detection of this control RNA serves as a positive control, verifying the functionality of the RNA purification as well as the detection using RT-qPCR. en Nuclease-resistant Control RNA, Control RNA Detection Mix (Cy5-labeled probe and primers), and LightCycler® Multiplex RNA Virus Master for monitoring the complete process of virus detection, from sample preparation to RT-qPCR. en The RNA Process Control Kit LSR is a tool to control for potential failures of sample preparation, amplification, detection, and handling errors. The product is intended for use with a variety of sample materials (e.g., blood, serum, stool, urine) and a variety of viral RNA targets.The RNA Process Control Kit LSR is intended for life science research only, not for use in diagnostic procedures. en Background InformationTwo major outcomes can occur during a typical detection workflow for viral RNA targets. The sample can be either positive or negative. Whereas a positive detection also verifies the functionality of workflow components, a negative test outcome could be due either to a true negative sample or to a failure of critical workflow components and thus be a false negative. To rule out the possibility of workflow failures, a positive control that is similar to the target material but inert to the target detection is supplied in this kit and can be used in the workflow.In order to prevent any cross-reaction with sample-derived nucleic acids or target-specific detection systems, the RNA Process Control Assay amplicon has been designed to have no significant homologies to any other known sequence. The RNA Process Control Detection Assay primers and probe are added in a low concentration to further lower any possible competition effects in multiplex reactions.The RNA Process Control concentrate is adjusted carefully to achieve a robust Cq value within one specified workflow. Different sample materials and workflows may require adjusted dilution of the RNA Process Control concentrate.The RNA Process Control Kit LSR is designed for the detection of an endogenous heterologous control (RNA Process Control) during a sample purification procedure and is compatible with: either a MagNA Pure System together with a dedicated reagent kit (for automated isolation),or a High Pure Nucleic Acid Isolation Kit (for manual isolation).How this Product WorksThe RNA Process Control Kit LSR provides a non-competitive internal control to monitor nucleic acid purification and detection processes in order to prevent false-negative results. The RNA Process Control working solution contains a constant amount of nuclease-resistant RNA(1) that can be added to a large variety of sample materials without impairing the purification of other sample intrinsic nucleic acid(2), such as from RNA viruses.The strictly monitored production and quality processes in conjunction with the easy-to-use workflow ensure that constant amounts of the control RNA are added to each and every sample.The RNA Process Control has a unique and completely artificial sequence that makes primer competition with any other target parameters less likely. The primer and the probe of the RNA Process Control are designed to only amplify and detect the unique sequence. The Cy5-labeled probe ensures that other target-specific assays with common dyes such as FAM, Yellow 555, HEX, or Red 610 can be combined in multiplex setups.Test PrincipleThe control is added to the sample material and co-purified with all other sample endogenous nucleic acids. After purification, the RNA Process Control as well as any target-specific parameter is detected in a RT-qPCR reaction.In monoplex reactions in single wells, the RNA Process Control must be positive.In multiplex reactions, the RNA Process Control detection must be positive in all target-negative samples. In target-positive samples, the RNA Process Control may be out-competed by the target-specific assay and a negative control result is allowed.Any failure of the RNA Process Control Detection Assay in target-negative samples indicates an erroneous purification/detection workflow and the sample must be retested.The key steps in the process are:RNA Process Control working solution is added to the sample material.The protective coat of the internal control is lysed during the extraction process, thus enabling co-purification of the control RNA with other sample endogenous nucleic acids.The sample eluates are used for RT-qPCR reactions.– The RNA Process Control Detection Assay specifically detects the internal control.– Lab-developed assays assess the status of other targets in the sample material.Since the RNA Process Control is added to all samples, its successful detection proves the correct sample processing as well as the functionality of the generic detection reagents. en