rdoe_global.search
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"Chapters": [ { "Language": "en", "Value": "The RNA Process Control purification step has various run times from 20 minutes (High Pure) up to 50 – 60 minutes for a typical run on the MagNA Pure 96 Instrument with 96 samples, depending on the protocol. The RNA Process Control detection can be used with a fast RT-qPCR protocol with run times of less than 65 minutes using the LightCycler® Multiplex RNA Virus Master on the LightCycler® 480 Instrument II or LightCycler® 96 Instrument.", "Country": "XG", "Code": "Typical run time", "Name": "Typical run time" }, { "Language": "en", "Value": "Store the kit at −15 to −25°C.", "Country": "XG", "Code": "Storage Conditions (Product)", "Name": "Storage Conditions (Product)" }, { "Language": "en", "Value": "Each component of the the RNA Process Control Kit LSR is function tested using the MagNA Pure 96 Instrument for nucleic acid purification and the LightCycler® 480 Instrument II for one-step RT-qPCR. ", "Country": "XG", "Code": "Quality Control", "Name": "Quality Control" }, { "Language": "en", "Value": "Suitable concentrations of hydrolysis probes range from 0.05 to 0.5 μM (final concentration in RT-qPCR). The recommended starting concentration is 0.25 μM each.
The optimal probe concentration is the lowest concentration that results in the lowest quantification cycle value (Cq) and adequate fluorescence dynamics for a given target concentration.
For a hydrolysis probe hybridization complex, the Tm of the hydrolysis probe has to be higher than the Tm of the primers.
", "Country": "XG", "Code": "Probe", "Name": "Probe" }, { "Language": "en", "Value": "The RNA Process Control Kit LSR contains a nuclease-resistant RNA that can be added at various steps during the nucleic acid purification of viral RNA detection workflows. Successful detection of this control RNA serves as a positive control, verifying the functionality of the RNA purification as well as the detection using RT-qPCR.", "Country": "XG", "Code": "Product Description", "Name": "Product Description" }, { "Language": "en", "Value": "Nuclease-resistant Control RNA, Control RNA Detection Mix (Cy5-labeled probe and primers), and LightCycler® Multiplex RNA Virus Master for monitoring the complete process of virus detection, from sample preparation to RT-qPCR.", "Country": "XG", "Code": "Positioning", "Name": "Positioning" }, { "Language": "en", "Value": "
Vial / BottleCapLabelFunction / DescriptionContent
1purpleRNA Process Control Kit LSR, RNA Process Control, conc.Nuclease-resistant RNA concentrate in storage buffer.6 vials, 10 μl each
2colorlessRNA Process Control Kit LSR, RNA Process Control DiluentRNA Process Control Diluent3 bottles, 17 ml each
3blueRNA Process Control Kit LSR,
RT Enzyme Solution, 200x conc.		LightCycler® Multiplex RNA Virus Master3 vials, 28 μl each
4redRNA Process Control Kit LSR,
RT-qPCR Reaction Mix, 5x conc.		LightCycler® Multiplex RNA Virus Master3 vials, 880 μl each
5yellowRNA Process Control Kit LSR, RNA Process Control Detection Assay, 20x conc.Primer/Probe Mix for detection of the RNA Process Control.6 vials, 120 μl each
6whiteRNA Process Control Kit LSR, Water, PCR GradeTo adjust the final reaction volume.12 vials, 1 ml each
", "Country": "XG", "Code": "Content", "Name": "Content" }, { "Language": "en", "Value": "The RNA Process Control Kit LSR is a tool to control for potential failures of sample preparation, amplification, detection, and handling errors. The product is intended for use with a variety of sample materials (e.g., blood, serum, stool, urine) and a variety of viral RNA targets.
The RNA Process Control Kit LSR is intended for life science research only, not for use in diagnostic procedures. ", "Country": "XG", "Code": "Applications", "Name": "Applications" }, { "Language": "en", "Value": "
Background Information
Two major outcomes can occur during a typical detection workflow for viral RNA targets. The sample can be either positive or negative. Whereas a positive detection also verifies the functionality of workflow components, a negative test outcome could be due either to a true negative sample or to a failure of critical workflow components and thus be a false negative. To rule out the possibility of workflow failures, a positive control that is similar to the target material but inert to the target detection is supplied in this kit and can be used in the workflow.

In order to prevent any cross-reaction with sample-derived nucleic acids or target-specific detection systems, the RNA Process Control Assay amplicon has been designed to have no significant homologies to any other known sequence. The RNA Process Control Detection Assay primers and probe are added in a low concentration to further lower any possible competition effects in multiplex reactions.
The RNA Process Control concentrate is adjusted carefully to achieve a robust Cq value within one specified workflow. Different sample materials and workflows may require adjusted dilution of the RNA Process Control concentrate.

The RNA Process Control Kit LSR is designed for the detection of an endogenous heterologous control (RNA Process Control) during a sample purification procedure and is compatible with: 
  • either a MagNA Pure System together with a dedicated reagent kit (for automated isolation),
  • or a High Pure Nucleic Acid Isolation Kit (for manual isolation).

How this Product Works
The RNA Process Control Kit LSR provides a non-competitive internal control to monitor nucleic acid purification and detection processes in order to prevent false-negative results. The RNA Process Control working solution contains a constant amount of nuclease-resistant RNA(1) that can be added to a large variety of sample materials without impairing the purification of other sample intrinsic nucleic acid(2), such as from RNA viruses.

  1. The strictly monitored production and quality processes in conjunction with the easy-to-use workflow ensure that constant amounts of the control RNA are added to each and every sample.
  2. The RNA Process Control has a unique and completely artificial sequence that makes primer competition with any other target parameters less likely. The primer and the probe of the RNA Process Control are designed to only amplify and detect the unique sequence. The Cy5-labeled probe ensures that other target-specific assays with common dyes such as FAM, Yellow 555, HEX, or Red 610 can be combined in multiplex setups.

Test Principle
The control is added to the sample material and co-purified with all other sample endogenous nucleic acids. After purification, the RNA Process Control as well as any target-specific parameter is detected in a RT-qPCR reaction.
  • In monoplex reactions in single wells, the RNA Process Control must be positive.
  • In multiplex reactions, the RNA Process Control detection must be positive in all target-negative samples. In target-positive samples, the RNA Process Control may be out-competed by the target-specific assay and a negative control result is allowed.
Any failure of the RNA Process Control Detection Assay in target-negative samples indicates an erroneous purification/detection workflow and the sample must be retested.

The key steps in the process are:
  1. RNA Process Control working solution is added to the sample material.
  2. The protective coat of the internal control is lysed during the extraction process, thus enabling co-purification of the control RNA with other sample endogenous nucleic acids.
  3. The sample eluates are used for RT-qPCR reactions.
    – The RNA Process Control Detection Assay specifically detects the internal control.
    – Lab-developed assays assess the status of other targets in the sample material.
Since the RNA Process Control is added to all samples, its successful detection proves the correct sample processing as well as the functionality of the generic detection reagents.", "Country": "XG", "Code": "Principle", "Name": "Principle" }, { "Language": "en", "Value": "Two major outcomes can occur during a typical detection workflow for viral RNA targets. The sample can be either positive or negative. Whereas a positive detection also verifies the functionality of workflow components, a negative test outcome could be due either to a true negative sample or to a failure of critical workflow components and thus be a false negative. To rule out the possibility of workflow failures, a positive control that is similar to the target material but inert to the target detection is supplied in this kit and can be used in the workflow.

In order to prevent any cross-reaction with sample-derived nucleic acids or target-specific detection systems, the RNA Process Control Assay amplicon has been designed to have no significant homologies to any other known sequence. The RNA Process Control Detection Assay primers and probe are added in a low concentration to further lower any possible competition effects in multiplex reactions.
The RNA Process Control concentrate is adjusted carefully to achieve a robust Cq value within one specified workflow. Different sample materials and workflows may require adjusted dilution of the RNA Process Control concentrate.

The RNA Process Control Kit LSR is designed for the detection of an endogenous heterologous control (RNA Process Control) during a sample purification procedure and is compatible with: 
  • either a MagNA Pure System together with a dedicated reagent kit (for automated isolation),
  • or a High Pure Nucleic Acid Isolation Kit (for manual isolation).

How this Product Works
The RNA Process Control Kit LSR provides a non-competitive internal control to monitor nucleic acid purification and detection processes in order to prevent false-negative results. The RNA Process Control working solution contains a constant amount of nuclease-resistant RNA(1) that can be added to a large variety of sample materials without impairing the purification of other sample intrinsic nucleic acid(2), such as from RNA viruses.

  1. The strictly monitored production and quality processes in conjunction with the easy-to-use workflow ensure that constant amounts of the control RNA are added to each and every sample.
  2. The RNA Process Control has a unique and completely artificial sequence that makes primer competition with any other target parameters less likely. The primer and the probe of the RNA Process Control are designed to only amplify and detect the unique sequence. The Cy5-labeled probe ensures that other target-specific assays with common dyes such as FAM, Yellow 555, HEX, or Red 610 can be combined in multiplex setups.

Test Principle
The control is added to the sample material and co-purified with all other sample endogenous nucleic acids. After purification, the RNA Process Control as well as any target-specific parameter is detected in a RT-qPCR reaction.
  • In monoplex reactions in single wells, the RNA Process Control must be positive.
  • In multiplex reactions, the RNA Process Control detection must be positive in all target-negative samples. In target-positive samples, the RNA Process Control may be out-competed by the target-specific assay and a negative control result is allowed.
Any failure of the RNA Process Control Detection Assay in target-negative samples indicates an erroneous purification/detection workflow and the sample must be retested.

The key steps in the process are:
  1. RNA Process Control working solution is added to the sample material.
  2. The protective coat of the internal control is lysed during the extraction process, thus enabling co-purification of the control RNA with other sample endogenous nucleic acids.
  3. The sample eluates are used for RT-qPCR reactions.
    – The RNA Process Control Detection Assay specifically detects the internal control.
    – Lab-developed assays assess the status of other targets in the sample material.
Since the RNA Process Control is added to all samples, its successful detection proves the correct sample processing as well as the functionality of the generic detection reagents.", "Country": "XG", "Code": "Background Information", "Name": "Background Information" }, { "Language": "en", "Value": "The RNA Process Control purification step has various run times from 20 minutes (High Pure) up to 50 – 60 minutes for a typical run on the MagNA Pure 96 Instrument with 96 samples, depending on the protocol. The RNA Process Control detection can be used with a fast RT-qPCR protocol with run times of less than 65 minutes using the LightCycler® Multiplex RNA Virus Master on the LightCycler® 480 Instrument II or LightCycler® 96 Instrument.", "Country": "XG", "Code": "Assay Time", "Name": "Assay Time" } ] } } ] }

RNA Process Control Kit LSR

Includes RNA Process Control, Control Assay, and multiplex one-step RT-qPCR master mix for monitoring the whole workflow, from nucleic acid purification to RT-qPCR.

RNA Process Control Kit LSR
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Instruments

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    ...
    DNA Process Control Kit
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    LightCycler<sup>®</sup> 480 SYBR Green I Master
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    LightCycler® 480 Multiwell Plate 96 white, 4 bar codes
    LightCycler<sup>®</sup> 8-Tube Strip Adapter Plate
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    LightCycler<sup>®</sup> 480 Multiwell Plate 96, clear
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    LightCycler<sup>®</sup> RNA Master HybProbe
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    MagNA Pure 24 Piercing Tool
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    MagNA Pure 24 Down Holder Frame
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    MagNA Pure 24 Liquid Waste Insert
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    MagNA Pure 24 MGP Set
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    MagNA Pure 96 System Fluid (Internal)
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    MagNA Pure 96 Reagent Rack
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    MagNA Pure External Lysis Buffer
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    MagNA Pure 96 Drop Catcher
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    RealTime ready Cell Lysis Buffer
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    FLOW Software Additional License
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