For Research Use Only. Not for use in diagnostic procedures. Others S.T.A.R. Buffer RUO S.T.A.R. Buffer 3.6.8.9.4.4 Stool Transport and Recovery buffer for stabilization, transport, and recovery of nucleic acids in stool specimens. S.T.A.R. Buffer 04038377018942 300 mL (3 × 100 mL) true S.T.A.R. 03335208001 60 preparations Reagents, kits en S.T.A.R. Buffer (Stool Transport and Recovery Buffer) is designed for the stabilization, transport, and recovery of nucleic acids from stool specimens for subsequent molecular analysis. S.T.A.R. Buffer is fully compatible with manual and automated extraction methods, such as isolation with the MagNA Pure LC Instrument or High Pure Extraction Kits.Use of the S.T.A.R. Buffer supports the reliability and sensitivity of the Nucleic Acid Detection from stool specimens by: Binding of inhibitory substances from stoolInactivation of NucleasesStabilization of Nucleic Acid during transport at room temperatureCompatibility to manual and automated extrac- tion methods (e.g. , HighPure, MagNA Pure LC)Research stool specimens are either analyzed for the presence of microbial specimens (bacteria, fungi, viruses, and parasites), or used for the isolation of genomic DNA (e.g ., detection of tumor cell DNA). However, stool specimens are known to contain a variety of enzyme inhibitors and nucleases that make it difficult to isolate and analyze nucleicacids from this highly vari- able specimen material. Therefore current procedures required that nucleic acids are either isolated directly after specimen collection, or the specimen is stored and shipped frozen. en For storage or transport, stool specimens are directly added to S.T.A.R. Buffer in a ratio of at least 1:3 (e.g ., for 1.5 mL of stool specimen [approx. 1.5 g] use 5 mL S.T.A.R. Buffer).S.T.A.R. Buffer is bacteriostatic and allows shipment and short term storage (up to 5days) of stool specimens at 15-25°C.For longer shipment and storage (more than 5 days) freeze the stool specimen in S.T.A.R. Buffer at -15 to -25°C.Add S.T.A.R. Buffer in a 1:3 ratio to specimens which are isolated right after the sampling procedure, in order to remove or inactivate PCR inhibitors.Note: Due to the bacteriostaticproperties of the S.T.A.R. Buffer, stool specimen can not be used for culture after transport in S.T.A.R. Buffer