{ "ProductData": { "ID": "3.6.8.9.4.4", "ProductType": "Others", "BrandName": "S.T.A.R. Buffer", "ProductNameAddition": "Stool Transport and Recovery buffer for stabilization, transport, and recovery of nucleic acids in stool specimens.", "RegulatoryDisclaimer1": "For Research Use Only. Not for use in diagnostic procedures.", "DisclaimerGroup1": "RUO", "RegulatoryDisclaimer2": null, "DisclaimerGroup2": null, "RegulatoryDisclaimer3": null, "SampleType": [ "Feces" ], "LicenseDisclaimers": [ ], "RelatedLinks": "", "Clone": "", "ControlTissue": [ "" ], "ISOtypes": "", "Species": [ "" ], "StainLocalization": [ "" ], "ProductNameGlobal": "S.T.A.R. 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Vial / BottleCapLabelFunction / DescriptionContent
1whiteS.T.A.R. BufferFor storage or transport of stool specimens
  • 3 bottles of 100 ml
", "Language": "en", "Country": "XG", "Code": "Content" }, { "Name": "Product Description", "Value": "S.T.A.R. Buffer (Stool Transport and Recovery Buffer) is designed for the stabilization, transport, and recovery of nucleic acids from stool specimens for subsequent molecular analysis. S.T.A.R. Buffer is fully compatible with manual and automated extraction methods, such as isolation with the MagNA Pure LC Instrument or High Pure Extraction Kits.

Use of the S.T.A.R. Buffer supports the reliability and sensitivity of the Nucleic Acid Detection from stool specimens by: 
  • Binding of inhibitory substances from stool
  • Inactivation of Nucleases
  • Stabilization of Nucleic Acid during transport at room temperature
  • Compatibility to manual and automated extrac- tion methods (e.g. , HighPure, MagNA Pure LC)

Research stool specimens are either analyzed for the presence of microbial specimens (bacteria, fungi, viruses, and parasites), or used for the isolation of genomic DNA (e.g ., detection of tumor cell DNA). However, stool specimens are known to contain a variety of enzyme inhibitors and nucleases that make it difficult to isolate and analyze nucleicacids from this highly vari- able specimen material. Therefore current procedures required that nucleic acids are either isolated directly after specimen collection, or the specimen is stored and shipped frozen.", "Language": "en", "Country": "XG", "Code": "Product Description" }, { "Name": "Applications", "Value": "For storage or transport, stool specimens are directly added to S.T.A.R. Buffer in a ratio of at least 1:3 (e.g ., for 1.5 mL of stool specimen [approx. 1.5 g] use 5 mL S.T.A.R. Buffer).

S.T.A.R. Buffer is bacteriostatic and allows shipment and short term storage (up to 5days) of stool specimens at 15-25°C.

For longer shipment and storage (more than 5 days) freeze the stool specimen in S.T.A.R. Buffer at -15 to -25°C.

Add S.T.A.R. Buffer in a 1:3 ratio to specimens which are isolated right after the sampling procedure, in order to remove or inactivate PCR inhibitors.

Note: Due to the bacteriostaticproperties of the S.T.A.R. Buffer, stool specimen can not be used for culture after transport in S.T.A.R. Buffer", "Language": "en", "Country": "XG", "Code": "Applications" }, { "Name": "Additional Equipment and Reagent Required", "Value": "
  • Sterile disposable polypropylene tube
  • Disposable spatula
  • Filter tips
  • Vortex mixer
  • Benchtop centrifuge
  • (Optional) Chloroform (p.A. grade, or better)
  • For nucleic acid purification, the following kits are recommended:
    - MagNA Pure 96 DNA and Viral NA Small Volume Kit
    - MagNA Pure 96 DNA and Viral NA Large Volume Kit
    - MagNA Pure 24 Total NA Isolation Kit
    - High Pure PCR Template Preparation Kit
    - High Pure Viral Nucleic Acid Kit
", "Language": "en", "Country": "XG", "Code": "Additional Equipment and Reagent Required" }, { "Name": "Applications (IFU)", "Value": "S.T.A.R. Buffer (Stool Transport and Recovery Buffer) is designed for the stabilization, transport, and recovery of nucleic acids from stool specimens for subsequent molecular analysis. S.T.A.R. Buffer is fully compatible with manual and automated extraction methods, such as isolation with the MagNA Pure 24 and 96 Instruments or High Pure Extraction Kits.
S.T.A.R. Buffer is added in a ratio of at least 1:3 to specimens which are isolated right after the sampling procedure, in order to remove or inactivate PCR inhibitors.", "Language": "en", "Country": "XG", "Code": "Applications (IFU)" }, { "Name": "Sample Materials", "Value": "Human or vertebrate animal stool, approx. 1.5 g per specimen (approx. the spoon size of a standard stool collection tube).", "Language": "en", "Country": "XG", "Code": "Sample Materials" }, { "Name": "Safety Information", "Value": "
Laboratory procedures
  • Handle all samples as if potentially infectious, using safe laboratory procedures. As the sensitivity and titer of potential pathogens in the sample material varies, the operator must optimize pathogen inactivation by the Lysis / Binding Buffer or take appropriate measures, according to local safety regulations.
  • Do not eat, drink or smoke in the laboratory work area.
  • Do not pipette by mouth.
  • Wear protective disposable gloves, laboratory coats and eye protection, when handling samples and kit reagents.
  • Do not contaminate the reagents with bacteria, virus, or nucleases. Use disposable pipets and nuclease free pipet tips only, to remove aliquots from reagent bottles. Use the general precautions described in the literature. Wash hands thoroughly after handling samples and reagents.
  • Finish each phase of the PCR/RT-PCR workflow before proceeding to the next phase. For example, you should finish PCR/RT-PCR sample preparation before starting PCR/RT-PCR set-up. Sample preparation, PCR/RT-PCR setup and the PCR/RT-PCR run itself should also be performed in separate locations.
Waste handling
  • Discard unused reagents and waste in accordance with country, federal, state, and local regulations.
  • Safety Data Sheets (SDS) are available online on dialog.roche.com, or upon request from the local Roche office.
", "Language": "en", "Country": "XG", "Code": "Safety Information" }, { "Name": "Product Purpose", "Value": "S.T.A.R. Buffer (Stool Transport and Recovery Buffer) is designed for the stabilization, transport, and recovery of nucleic acids from stool specimens for subsequent molecular analysis. S.T.A.R. Buffer is fully compatible with manual and automated extraction methods, such as isolation with the MagNA Pure LC Instrument or High Pure Extraction Kits.

Use of the S.T.A.R. Buffer supports the reliability and sensitivity of the Nucleic Acid Detection from stool specimens by: 
  • Binding of inhibitory substances from stool
  • Inactivation of Nucleases
  • Stabilization of Nucleic Acid during transport at room temperature
  • Compatibility to manual and automated extrac- tion methods (e.g. , HighPure, MagNA Pure LC)

Research stool specimens are either analyzed for the presence of microbial specimens (bacteria, fungi, viruses, and parasites), or used for the isolation of genomic DNA (e.g ., detection of tumor cell DNA). However, stool specimens are known to contain a variety of enzyme inhibitors and nucleases that make it difficult to isolate and analyze nucleicacids from this highly vari- able specimen material. Therefore current procedures required that nucleic acids are either isolated directly after specimen collection, or the specimen is stored and shipped frozen.", "Language": "en", "Country": "XG", "Code": "Product Purpose" } ] } } ] }

S.T.A.R. Buffer

Stool Transport and Recovery buffer for stabilization, transport, and recovery of nucleic acids in stool specimens; compatible with extraction using the MagNA Pure LC Instrument

RUO For Research Use Only. Not for use in diagnostic procedures.
S.T.A.R. Buffer

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