For life science research only. Not for use in diagnostic procedures. Others Transcriptor First Strand cDNA Synthesis Kit LSR Transcriptor First Strand cDNA Synthesis Kit 3.6.8.39.1.2 04 897 030 001 4 897 030 001 04897030001 4897030001 04897030001 Transcriptor cDNA Synth. Kit 200 react. Transcriptor First Strand cDNA Synthesis Kit 04038377024554 Reagents, kits 1 kit 200 reactions false 04 379 012 001 4 379 012 001 04379012001 4379012001 04379012001 Transcriptor First Strand cDNA Synt. Kit Transcriptor First Strand cDNA Synthesis Kit 04038377020686 Reagents, kits 1 kit 50 reactions, including 10 control reactions false 04 896 866 001 4 896 866 001 04896866001 4896866001 04896866001 Transcriptor cDNA Synth. Kit 100 react. Transcriptor First Strand cDNA Synthesis Kit 04038377024547 Reagents, kits 1 kit 100 reactions false The Transcriptor First Strand cDNA Synthesis Kit is designed to reverse transcribe RNA (mRNA, total RNA, viral RNA, and in vitro transcribed RNA) from a variety of sources for the following applications:Study gene expression levels, via two-step RT-PCR, using qualitative RT-PCR on conventional thermal cyclers or quantitative RT-PCR on the LightCycler® Carousel-Based System, the LightCycler® 480 System, or other real-time PCR instruments.Generate cDNA libraries with large and full-length inserts.Clone genes of interest.The kit contains all components required for cDNA reactions for use with conventional thermal cyclers and real-time PCR instruments. In addition, the 50-reaction pack size includes 10 control reactions. en The Transcriptor First Strand cDNA Synthesis Kit provides all reagents required for first-strand cDNA synthesis reactions up to 14kb on all real-time instruments and conventional thermal cyclers. Three different priming methods can be used. The enclosed anchored-oligo(dT)18 primer is designed to bind at the beginning of the poly(A) tail and guarantees full-length cDNA synthesis.Transcriptor Reverse Transcriptase - the core component of the kit - has RNA-directed DNA polymerase activity, DNA-dependent DNA polymerase activity, unwinding activity, and RNase H activity that digests RNA in RNA:DNA hybrids. The latter circumvents the need to perform an additional time-consuming RNase H incubation step after reverse transcription. This shortens the reaction time and reduces costs.Thermostable Protector RNase Inhibitor is included in the kit to protect RNA from degradation at high reaction temperatures. en Using the Transcriptor First Strand cDNA Synthesis Kit, RNA is reverse transcribed into single-stranded cDNA, which can be used directly for subsequent PCR with gene-specific primers on conventional thermal block cyclers and real-time PCR instruments (e.g. the LightCycler® Carousel-Based System, the LightCycler® 480 Instrument or other real-time PCR instruments), or for other downstream applications.Transcriptor Reverse Transcriptase is a recombinant reverse transcriptase expressed in E. coli. The enzyme has RNA-directed DNA polymerase activity, DNA-dependent DNA polymerase activity, unwinding activity and, very importantly, RNase H activity that degrades RNA in RNA:DNA hybrids. Thus, there is no need to perform an additional time-consuming RNase H incubation step after reverse transcription, significantly shortening the reaction time. Single-stranded RNA as well as ssDNA are accepted as template and are reverse transcribed in the presence of a primer.Transcriptor Reverse Transcriptase is recommended for RT-PCR because of its high sensi-tivity in connection with very high thermostability: The enzyme is able to synthesize long cDNA products (up to 14 kb) and can be used at temperatures up to +65°C. Due to its high thermostability, Transcriptor Reverse Transcriptase is recommended for GC-rich templates with high secondary structure without the need to include additives in the reaction.The kit provides all reagents required for performing first strand cDNA synthesis reactions from RNA. For priming, three different primer systems may be used as shown in Figure 5. Two cDNA synthesis primers are already provided with the kit: random hexamer primers and an anchored-oligo(dT)18 primer. The latter is designed to bind at the very beginning of the poly(A) tail to generate full-length cDNA and to prevent priming from internal sites of the poly(A) tail. Although 5'-ends of long mRNAs can be underrepresented, this priming method is preferred for most applications.. The use of random hexamer primers provides priming throughout the length of RNA for uniform representation of all RNA sequences and, in addition, allows reverse transcription of RNA molecules that do not have a poly(A) tail. en

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Transcriptor First Strand cDNA Synthesis Kit

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