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High-Performance Real-Time PCR with LightCycler® Systems

Get precise, consistent results, for any throughput or budget, with our range of instruments, assays, reagents and disposables

High performance real-time PCR:

More than a decade of continuous qPCR innovation with the LightCycler® Systems.

Roche continues to innovate, and with that comes a dedication to the scientists who use the LightCycler® system for their experimental needs. Benefit from Roche's experience in real-time PCR and achieve precise, consistent results with a full range of instruments, reagents, assays, and disposables to fit any laboratory throughput or budget. 

Choose the LightCycler® System that's right for you

 

 

LightCycler® 96 Instrument

See qPCR in a whole new way

Generate data you can trust.

Ideal for all levels of experience, the LightCycler® 96 Instrument provides an ideal combination of temperature homogeneity and data reproducibility. Comprehensive programming options, a large touchscreen, and intuitive analysis software that is accessible to any user make it easy to obtain publication-ready results.
 

Learn More

Discover which real-time PCR System is right for you.

The tools for your research start here.

  LightCycler® 2.0* System LightCycler® 96
System
LightCycler® 480 System LightCycler® 1536 System
Reactions per Run 32 96 96/384 1536
PCR Reactions Volume 20 µl 10 - 50 µl 5-20 µl ⁄ 10 - 100 µl 0.5 - 2 µl
Run Time <30 min <40 min <40 min (384) ⁄ <60 min (96) <50 min
Disposables Glass capillaries 96-multiwell plates
8-tube strips
96/384-multiwell plates
8-tube strips
1536-multiwell
plates
Multiplexing Capability 5x 4x 6x 2x
Automation-Capable No No Yes Yes
Light Source Blue LED  High-Intensity LED  High-Intensity white LED Xenon Lamp
         

* LightCycler® 2.0 Instrument conforms to the European directive for in vitro diagnostic medical devices 98/79/EC. For in vitro diagnostic use.

Order qPCR instruments

Order qPCR instruments

Request a quote for a real-time PCR system.
 

Request Quote
Order qPCR reagents

Order qPCR reagents

Optimized reagents for each application.
 

More Info
Order qPCR assays

Order qPCR assays

Easily design your qPCR assays or function-tested custom qPCR panels.

More Info

Innovative qPCR Instruments.

Generate results you can trust.

Amplify your success in real-time PCR applications with the LightCycler® Instruments. Choose the system that best suits your needs, with flexible sample volumes and throughputs ranging from 32 to 1,536 samples per run. All of our instruments provide outstanding temperature uniformity and homogeneity, as well as unbiased data capture, enabling you to quickly generate results that are accurate and reproducible.

LightCycler® 96 System

LightCycler® 96 System

See qPCR in a whole new way.
96-well throughput

Ideal for all levels of experience, the LightCycler® 96 Instrument provides an optimal combination of temperature homogeneity and data reproducibility. Comprehensive programming options, a large touchscreen, and intuitive analysis software make it easy to obtain publication-ready results.

 

Learn More

LightCycler® 480 System

LightCycler® 480 System

Versatile for experienced users.
96- to 384-well throughput

The LightCycler® 480 System is a high-performance, flexible throughput qPCR platform (interchangeable 96- or 384-well blocks) that provides various methods for gene detection, gene expression analysis, genetic variation analysis, and pathogen detection.

 

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LightCycler® 1536 System

LightCycler® 1536 System

High throughput - redefined.
1536-well throughput

The LightCycler® 1536 Instrument is a rapid, plate-based, thermal block cycler with integrated real-time, online detection capabilities. Take advantage of the first plate-based real-time qPCR instrument that provides 1,536 data points in a single PCR run in less than 50 minutes.

 

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LightCycler®  2.0 System

LightCycler® 2.0 System

A proven standard of excellence.
32-well throughput


This LightCycler® Carousel-Based System has repeatedly set the standard for accurate and fast real-time PCR. It was the first system to introduce hybridization probes, true melting curve analysis, automated absolute quantification, relative quantification with efficiency correction, and more.

LightCycler® 2.0 Instrument conforms to the European directive for in vitro diagnostic medical devices 98/79/EC. For in vitro diagnostic use.

Learn More

qPCR Reagents

Designed to maximize the performance of your system.

Rely on Roche proprietary PCR enzymes and formulations to consistently deliver exceptional sensitivity and specificity for your real-time PCR assays.
Choose the reagent that's best for your experiments on:

LightCycler® Instruments

Non-Roche qPCR instruments

 

LightCycler® Multiplex Master Mix

LightCycler® Multiplex Masters

Made to discover more.

Bring your qPCR to the next level with the new 5x concentrated multiplex master mixes. Detect a greater number of targets per reaction, work more efficiently, and maximize your valuable samples.

 

Learn More

EvoScript_LightCycler

LightCycler® EvoScript Masters

Witness the evolution of RT-qPCR.

Sensitive, specific and one-vial EvoScript master mixes turn even the smallest clues into measurably better results, ensuring no evidence gets lost in transcription.

 

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Reagent Selection Guide

Reagent Selection Guide

Your online search assistant

Not sure which kit is best for you? With the new Roche Reagent Selection guide, you can easily find which real-time PCR reagents are right for your applications.

 

Launch the Guide

qPCR Reagents for Roche Instruments

  FastStart Essential DNA Probes Master FastStart Essential DNA Green Master LightCycler® 480 Probes Master LightCycler® 480 SYBR® Green I Master LightCycler® 480 RNA Master Hydrolysis Probes LightCycler® 480 HRM Master LightCycler® Multiplex RNA Virus Master LightCycler® Multiplex DNA Master
Applications Gene expression Gene expression Gene expression Gene expression Gene expression High resolution melting Multiplex pathogen detection Multiplex pathogen detection
Starting Material DNA DNA DNA DNA RNA DNA Viral RNA Pathogen DNA
Chemistry Hydrolysis probes SYBR® Green I    Hydrolysis probes SYBR® Green I Hydrolysis probes ResoLight Dye Hydrolysis probes Hydrolysis probes
Instrument Capability LightCycler® 96 Instrument LightCycler® 96 Instrument LightCycler® 480 Instrument LightCycler® 480 Instrument LightCycler® 96 & LightCycler® 480 Instruments LightCycler® 96 & LightCycler® 480 Instruments LightCycler® 96 & LightCycler® 480 Instruments LightCycler® 96 & LightCycler® 480 Instruments

qPCR Reagents for Non-Roche Instruments

Roche Master Mix FastStart Universal Probe Master Mix (ROX) FastStart Universal SYBR® Green Master Mix (ROX) FastStart TaqMan Probe Master FastStart SYBR® Green Master EagleTaq Universal Master Mix (ROX)
Applications Gene expression Gene expression Gene expression Gene expression
Gene expression, Genotyping
Starting Material DNA DNA DNA
DNA DNA
Chemistry Hydrolysis probes SYBR® Green I    Hydrolysis probes SYBR® Green I Hydrolysis probes
ROX Yes Yes No No Yes
Instrument Capability non-Roche instruments requiring ROX such as ViiA7, StepOne Plus, ABI 7500, ABI 7900 HT. non-Roche instruments requiring ROX such as ViiA7, StepOne Plus, ABI 7500, ABI 7900 HT.
non-Roche instruments not requiring ROX such as Bio-Rad CFX 96 Touch, RotorGene Q. non-Roche instruments not requiring ROX such as Bio-Rad CFX 96 Touch, RotorGene Q. non-Roche instruments requiring ROX such as ViiA7, StepOne Plus, ABI 7500, ABI 7900 HT.
LightCycler® 1536 System Reagents

LightCycler® 1536 System Reagents

Downscale reagent volumes and accelerate the workflow.

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LightCycler® Carousel System Reagents

LightCycler® Carousel System Reagents

Optimal reagents for rapid reactions in capillaries.
 

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Reverse Transcription Kits

Reverse Transcription Kits

For robust and unbiased cDNA synthesis.
 

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Genomic Workflow Publication Database

Genomics Workflow Publications

The Genomic Workflow Publication Database is your source for relevant, reliable information specific to your research applications.

Explore Database
Universal ProbeLibrary

Universal ProbeLibrary

Easily design your qPCR assay to analyze over 5 million transcripts of any sequenced organism.


Configure Assay
RealTime ready

RealTime ready

Function-tested qPCR single assays or custom panels, for human, mouse or rat targets of your choice.
 

Configure Panel

qPCR Assays and Panels

Easy to design. Ready to run.

Choose from a variety of Roche probe-based qPCR assay solutions for your gene expression needs. Opt for universal probes for affordable and easy-to-design assays. Or select function-tested single assays and customized panels to skip design and optimization steps. Rely on Roche qPCR assay solutions to generate reliable and consistent real-time PCR results, along with complete assay information to facilitate compliance with the MIQE guidelines.

Choose the assay solution that's best for you

Lightmix for qPCR

LightMix Modular Pathogen Detection Assay*

Ready to use assays designed for multiplexing.

Choose from an extensive range of assays for pathogen detection. Compatibility with Roche reagents and instruments enables seamless assay integration into your workflow.

*Manufactured by TIB MOLBIOL GmBH, exclusively distributed by Roche

 

Create Your Panel

Universal Probe Library for qPCR

Universal ProbeLibrary

Cost-effective, probe-based assays.

Experience how fast and easy it is to design a specific, cost-effective, hydrolysis probe-based qPCR assay in 3 easy steps using our free online software.

 

Get Started

RealTime ready Assays

RealTime ready Assays

Function-tested probe-based assays.

Skip time-consuming assay design and optimization steps with the function-tested RealTime ready qPCR assays for gene expression for human, mouse, rat or other species. Receive ready-to-use probe-based assays, compatible with any real-time PCR instrument

 

Design Assay

RealTime ready Panel

RealTime ready Custom Panels

Function-tested qPCR assay Panels.

Create your own gene expression panels with your targets of choice on LightCycler® Plates (96- or 384-well). All assays are function-tested and pre-plated for the upmost convenience and quick time to results.

 

Configure Panel

Probe-based qPCR assay solutions available from Roche


Assay
LightMix
 Modular Assays
Universal
ProbeLibrary
Single Probes
Universal
ProbeLibrary Library
RealTime ready
Single Assays
RealTime ready
Focus Panels
RealTime ready
Custom Panels
Format Predesigned assays for detection of infectious
disease pathogens
(2 vials)
Single probe
(2 vials)
90 most common
probes for human
targets (90 vials)
Custom Assay,
single tube
Pre-configured,
pre-plated
(96- or 384-well)
Custom, pre-plated
(96- or 384-well)
Included
Components
Probe, Primers and
Positive Control
Probe 90 probes Probe and Primers Probes, Primers
and Plates
Probes, Primers
and Plates
Validation Function-tested In silico In silico Function-tested Function-tested Function-tested
Assay
Information
Protocol available
from Roche
Complete assay information provided with all Roche qPCR assays
Instrument Compatibility LightCycler® 480 and 
cobas z 480
qPCR Instruments
 detecting FAM
qPCR Instruments
detecting FAM
qPCR Instruments
detecting FAM
LightCycler® 480
LightCycler® 96
LightCycler® 480
LightCycler® 96
RealTime ready Pre-Amp cDNA Master and Pre-Amp Pirmer pools

Working with limited amounts of RNA?

Learn more about RealTime ready Pre-Amp cDNA Master and Pre-Amp pirmer pools.

Learn More
Reference Gene Assays

Reference Gene Assays

Easily multiplex with your target UPL assay.                                                                         

Find Assays
Reference Gene Panel

Reference Gene Panel

Identify the reference genes with the least variability for your test system.

Find Products

LightCycler® Consumables

Designed to maximize performance.

LightCycler® Multiwell Plates

LightCycler® Multiwell Plates

Optimal fit and performance.

Fully exploit the advanced thermal and optical characteristics of your LightCycler® 96 or 480 Systems with tailor-made LightCycler® Multiwell Plates to allow optimal heat transfer and optical compatibility.

 

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LightCycler® 8-Tube Strips

LightCycler® 8-Tube Strips

Ideal consumable for low throughput.

Use the LightCycler® 8-Tube Strips as plastics disposables that match exactly your LightCycler® 96 or 480 Systems block geometry for optimal fit and real-time PCR results.

 

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LightCycler® Capillaries

LightCycler® Capillaries

Optimized for Carousel Systems.

Maximize qPCR performance with optimal heat transfer by relying on the renowned LightCycler® Capillaries for all LightCycler® Carousel Systems.

 

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LightCycler® Multiwell Plates

Want to simplify plate tracking?

Rely on LightCycler® Multiwell Plates with 4 barcodes for tracking and automation.

 

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LightCycler® 1536 Multiwell Plates

LightCycler® 1536 Multiwell Plate

Increase your throughput with 1,536 data points per run.

Find Products