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LightCycler® FastStart DNA MasterPLUS HybProbe

Easy-to-use hot start reaction mix for PCR using the LightCycler® Carousel-Based System.

For general laboratory use.

Product No. Pack Size
03515575001 96 x 20 µL reactions
Product No. Pack Size
03515567001 480 x 20 µL reactions
Product No. Pack Size
03752178001 1920 x 20 µL reactions

To order these products, please contact your Roche order management team.

Easy-to-use Hot Start Reaction Mix for PCR using HybProbe probes with the LightCycler® Carousel-Based System.

This  master mixe offers convenience and ease-of-use because the addition of MgCl2 to the reaction mixture is not necessary, thus avoiding time-consuming optimization steps.
The new buffer formulation results in increased PCR robustness.


  • LightCycler® FastStart Enzyme
  • LightCycler® FastStart Reaction Mix
  • Water, PCR Grade

LightCycler® FastStart DNA MasterPLUS HybProbe is an easy-to-use hot start reaction mixes for highly sensitive PCR applications in glass capillaries, using HybProbe probes as detection format and an ideal master mix for performing two-step RT-PCR for gene expression analysis.
Note: LightCycler® FastStart DNA MasterPLUS HybProbe can be used in conjunction with Uracil DNA Glycosylase, heat-labile for carryover prevention during PCR.

The hot start feature of the master mix is provided by FastStart Taq DNA Polymerase, a chemically modified Taq DNA polymerase, which is inactive at room temperature and becomes activated at high temperatures (i.e., a preincubation step at +95°C for 5 – 10 minutes).
The unique LightCycler® HybProbe format is based on the principle of fluorescence resonance energy transfer (FRET). Two sequence-specific oligonucleotide probes, labeled with different dyes (donor and acceptor), are added to the reaction mix in addition to PCR primers. HybProbe probes hybridize to the target sequences on the amplified DNA fragment during the annealing phase in a head-totail arrangement, thereby bringing the two dyes into close proximity. The donor dye (fluorescein) is excited by the blue light LED source and the energy emitted by the donor dye excites the acceptor dye attached to the second HybProbe probe, which then emits fluorescent light at a different wavelength. The amount of fluorescence is directly proportional to the amount of target DNA generated during the PCR process.
All LightCycler® Master Mixes have been specifically developed to function in glass capillaries and provide optimal performance and sensitivity.

Product Citations:
Hatch, E. et al (2006) A rapid genotyping method for the vitamin K epoxide reductase complex subunit 1 (VKORC1) gene. J Thromb Haemost 4, 1158-9.
Skogseth H. et al (2006) Urokinase plasminogen activator receptor (uPAR) expression is reduced by tyrosine kinase inhibitors. APMIS 114, 307-13.
Burggraf, S. et al (2004) Simple Technique for Internal Control of Real-Time Amplification Assays. Clinical Chemistry 50:5, 819-825.
Prior, F. et al (2006) Accurate Determination of Zygosity in Transgenic Rice by Real-time PCR Does Not Require Standard Curves or Efficiency Correction. Transgenic Research 15(2), 261-265.
Ohl, F. et al (2006) Identification and Validation of Suitable Endogenous Reference Genes for Gene Expression Studies of Human Bladder Cancer. Journal of Urology 175, 1915-1920.


Function test: The LightCycler® FastStart DNA MasterPLUS HybProbe is function tested using the LightCycler® Carousel-Based System Instruments.