MagNA Pure 96 DNA and Viral NA Large Volume Kit

Ready-to-use reagents in two prefilled sealed trays for purifying nucleic acids from up to 4,000 μL sample volume using the MagNA Pure 96 Instrument.
For in vitro diagnostic use.

Product No. Pack Size
06374891001 288 isolations

To order these products, please contact your Roche order management team.

Keywords: Kits & Reagents

Purification of genomic DNA from:

  • Up to 1000 µl whole blood (with or without external lysis option)
  • Up to 1x106 culture cells
  • Up to 25 mg fresh tissue

Purification of Viral DNA and RNA, and bacterial and fungal DNA from:

  • Up to 500 µl mammalian whole blood, serum, plasma, urine, sputum, swabs, stool, bronchoalveolar lavage (BAL), cerebrospinal fluid (CSF), and bacterial cultures (with or without external lysis option)
  • Up to 1,000 µl mammalian whole blood, serum, plasma, urine, swabs, bronchoalveolar lavage (BAL), cerebrospinal fluid (CSF), and bacterial cultures using a batch of 48
  • For cfNA protocols, we use 2,000 or 4,000 µL of plasma

For more detailed product information, please contact your local Roche representative.
To isolate DNA and viral nucleic acids from smaller volume samples of up to 200 µl, choose the MagNA Pure 96 DNA and Viral NA Small Volume Kit.

  • Treat all samples like urgent samples using short purification times of  70 to 100 minutes for 96 large volume samples.
  • Obtain high yields of pure genomic DNA or viral DNA and RNA.
  • Experience flexible automated purification from a broad range of sample materials.
  • Save time during instrument setup using a minimal number of ready-to-use reagents.
  • Minimize pipetting steps and contamination risk using reagents prefilled in containers.

Contents

Three sets of two different prefilled reagent trays, each containing the following buffers:

  • 3 x Reagent Tray 1 (Wash Buffer I, Lysis/Binding Buffer)
  • 3 x Reagent Tray 2 (Proteinase K, Elution Buffer, Wash Buffer III)
    and
  • 6 x Bottle 1 with Magnetic Glass Particles (MGPs, 18 ml each) and self-sealable lid

Each set can be used up to two times. 

The kit is used to purify genomic DNA from up to 500 µL mammalian whole blood, plasma and serum or up to 1,000 µL whole blood, plasma, serum using a batch of 48 samples, up to 25 mg fresh-frozen tissue or up to 1 x 106 cultured cells using the MagNA Pure 96 System. It can also be used to purify viral DNA and RNA, and bacterial and fungal DNA from up to 500 µl mammalian whole blood, serum, plasma, urine, sputum, swabs, stool, bronchoalveolar lavage (BAL), cerebrospinal fluid (CSF), and bacterial cultures, or from up to 1000 µl mammalian whole blood, serum, plasma, urine, swabs, bronchoalveolar lavage (BAL), cerebrospinal fluid (CSF), and bacterial cultures using a batch of 48. External lysis is optional. Purified nucleic acids can be used directly for PCR and RT-PCR using LightCycler® 96 System, the LightCycler® 480 System, and other standard thermal block and real-time PCR cyclers.

Detection of bacterial, fungal and viral NA in routine microbiological sample types on the MagNA Pure 96 System article. Download here.

Package inserts

Access package inserts through your country’s Roche Diagnostics website.