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MagNA Pure 96 Instrument

High-throughput robotic workstation for fully automated purification of nucleic acids from up to 96 samples.

For in vitro diagnostic use.

Product No. Pack Size
06541089001 1 instrument, control unit and accessories

To order these products, please contact your Roche order management team.

The MagNA Pure 96 System purifies DNA, RNA, and viral nucleic acids from a wide range of starting materials using magnetic glass particle technology.
 

Preprogrammed software protocols are tailored to a diversity of sample requirements. Resulting nucleic acids are suitable for a broad range of downstream applications.
 

The MagNA Pure 96 System effortlessly processes up to 96 samples in less than one hour using barcoded, prefilled trays with ready-to-use reagents.
 

Experience seamless data management using intuitive features, such as the link to a laboratory information management system (LIMS).

 

 

 

Feature:

 

Benefit:

Speed and throughput

  • Approximately 1 hour for processing a 96 sample run
  • Multiple sample types can be processed in one run

Quick time-to-result for clinicians and patients

Convenience
  • Only two kits needed to cover various diagnostic applications
  • Few reagents and consumables needed
  • Connectivity (LIMS and bidirectional file sharing)

Minimized error risk
Low training effort & hands-on time

Robustness

  • Insusceptible against tested substance interferences
  • Very stable against cross contamination

Confidence & trust in your results

Performance
  • Broad range of sample materials
  • Excellent precision, purification efficiency and linear range
  • Minimal lot-to-lot variation

One solution for various needs

Service and support

  • Globally, approximately 3,500 employees at Roche Diagnostics are dedicated to service and support
  • Roche is a leading supplier of IVD products. No other company has more experience in molecular diagnostics

Minimum downtime
Cost effectiveness

The MagNA Pure 96 System is a high-throughput instrument for automated nucleic acid purification. Typical run duration is less than one hour for 96 standard volume samples. Applications are very flexible, due to a diversity of software purification protocols using barcoded, prefilled trays with ready-to-use reagents.

MagNA Pure 96 kits are designed profiting from our more than ten year expertise in magnetic glass particle technology. We have streamlined the purification process to a very small number of reagents, consumables and steps.

Optimized preprogrammed purification protocols ensure routine sample handling and high quality, reproducible nucleic acid yields. Choose the appropriate purification protocol for your sample material and target nucleic acid:

 Target: Bacterial, fungal and viral NA
Protocol name Input sample type and sample volumes Elution   volume Run time**
(h:mm)
 Whole  blood Plasma   (citrate)   Plasma   (EDTA)    Serum  Swabs  BAL  Sputum   CSF   Urine   Stool   Cultured  cells Fresh-frozen  tissue   FFPET 
Pathogen Universal 2001 200 μL 200 μL 200 μL 200 μL 200 μL 200 μL 200 μL 200 μL 200 μL 200 μL       50,
100 μL
1:00
Viral NA Plasma SV1     50, 100,
200 μL
                    50,
100 μL
1:00
Viral NA Plasma ext lys SV1
Protocol optimized with external lysis reagent
100, 200 μL 100, 200 μL 100, 200 μL 100, 200 μL                   50,
100 μL
1:00
Viral NA Universal SV1 50, 100, 200 μL 50, 100, 200 μL   50, 100, 200 μL                   50,
100 μL
1:00
Pathogen Universal 5002
500 μL
500 μL
500 μL 500μL 500 μL 500 μL 500 μL 500 μL 500 μL 500 μL       50,
100 μL
1:30
Pathogen Universal 10002
1000 μL 1000 μL 1000 μL 1000 μL 1000 μL 1000 μL 1000 μL 1000 μL 1000 μL 1000 μL       50,
100 μL
1:40
Viral NA Plasma LV2       500 μL
                    50,
100 μL
1:10
Viral NA Plasma LV 10002       1000 μL
                    50,
100 μL
1:20

Viral NA Plasma ext lys LV2
Protocol optimized with external lysis reagent

  500 μL
500 μL
500 μL
                  50,
100 μL
1:10
Viral NA Universal LV2   500 μL
  500 μL
                  50,
100 μL
1:30
Viral NA Universal LV 10002     1000 μL
  1000 μL
                  50,
100 μL
1:30

1 MagNA Pure 96 DNA and Viral NA Small Volume Kit (cat. no. 06 543 588 001)
2 MagNA Pure 96 DNA and Viral NA Large Volume Kit (cat. No. 06 374 891 001)

 *BAL - Bronchoalveolar Lavage
 **These times are approximate and should only be used as a guidance.

To get better results, Roche provides various External Lysis Buffers: MagNA Pure cfNA Buffer Set ( Cat. No. 07 794 398 001), MagNA Pure 96 External Lysis Buffer (Cat. No. 06 374 913 001), MagNA Pure 96 Bacterial Lysis Buffer (Cat. No. 06 374 921 001), and MagNA Pure 96 DNA Tissue Lysis Buffer (Cat. No. 06 640 702 001). Please refer to the Instructions for Use manual for more details.
  Target: Genomic DNA
 Protocol name  Input sample type and sample volumes Elution   volume Run time**
(h:mm) 
 Whole blood Plasma (citrate)  Plasma (EDTA)  Serum  Swabs  BAL  Sputum   CSF   Urine   Stool   Cultured cells Fresh-frozen tissue  FFPET 
DNA Blood ds SV1, 4
Protocol optimized for double-stranded DNA and NGS
200 μL                         50,
100 μL
1:00
DNA Blood SV1 50, 100, 200 μL
                        50,
100 μL
1:00
DNA Blood ext lys SV1
Protocol optimized with external lysis reagent
50, 100, 200 μL
50, 100, 200 μL
50, 100, 200 μL
50, 100, 200 μL
                  50,
100 μL
0:50
DNA Cells SV1                     Up to 5x105 cultured cells in 200 μL PBS     100 μL 1:00
DNA Tissue SV1                     Up to 5mg in 200 μL     100, 200 μL 1:20
DNA FFPE SV1                         Lysate in 200 μL 50, 100 μL 1:00
DNA Blood LV2 500 μL                         100,
200 μL
1:20
DNA Blood LV 10002 1000 μL                         100,
200 μL
1:30
DNA Blood ext lys LV2
Protocol optimized with external lysis reagent
500 μL
500 μL
500 μL
500 μL
                  100,
200 μL
0:50

DNA Cells LV1

                    Up to 1x106 cultured cells in 200 μL PBS     100,
200 μL
1:20
DNA Tissue LV2                     Up to 25 mg
in 200 μL
    200 μL 1:20

 1 MagNA Pure 96 DNA and Viral NA Small Volume Kit (cat. no. 06 543 588 001)
 2
MagNA Pure 96 DNA and Viral NA Large Volume Kit (cat. No. 06 374 891 001)
 4 New Protocols

 *BAL - Bronchoalveolar Lavage
 **These times are approximate and should only be used as a guidance.
To get better results, Roche provides various External Lysis Buffers: MagNA Pure cfNA Buffer Set ( Cat. No. 07 794 398 001), MagNA Pure 96 External Lysis Buffer (Cat. No. 06 374 913 001), MagNA Pure 96 Bacterial Lysis Buffer (Cat. No. 06 374 921 001), and MagNA Pure 96 DNA Tissue Lysis Buffer (Cat. No. 06 640 702 001). Please refer to the Instructions for Use manual for more details.
 Target: Cell-free NA
 Protocol name  Input sample type and sample volumes Elution   volume Run time**
(h:mm)
 Whole  blood Plasma (citrate)  Plasma  (EDTA)  Serum   Swabs  BAL  Sputum   CSF   Urine   Stool   Cultured  cells Fresh-frozen tissue  FFPET 
cfNA ss 20002 
Protocol optimized for single-stranded DNA
    2000 μL
                    50,
100 μL
2:00
cfNA ss 40002 
Protocol optimized for single-stranded DNA
    4000 μL
                    50,
100 μL
2:20
cfNA ds 20002 
Protocol optimized for double-stranded DNA
    2000 μL
                    100,
200 μL
2:20
cfNA ds 40002 
Protocol optimized for double-stranded DNA
    4000 μL
                    100,
200 μL
2:50
MagNA Pure 96 DNA and Viral NA Large Volume Kit (cat. No. 06 374 891 001)

*BAL - Bronchoalveolar Lavage
**These times are approximate and should only be used as a guidance.

To get better results, Roche provides various External Lysis Buffers: MagNA Pure cfNA Buffer Set ( Cat. No. 07 794 398 001), MagNA Pure 96 External Lysis Buffer (Cat. No. 06 374 913 001), MagNA Pure 96 Bacterial Lysis Buffer (Cat. No. 06 374 921 001), and MagNA Pure 96 DNA Tissue Lysis Buffer (Cat. No. 06 640 702 001). Please refer to the Instructions for Use manual for more details.
 Target: Total RNA
Protocol name  Input sample type and sample volumes Elution volume Run time**
(h:mm)
 Whole  blood Plasma   (citrate)   Plasma  (EDTA) Serum  Swabs BAL*  Sputum   CSF   Urine  Stool  Cultured cells Fresh-frozen tissue   FFPET 
Cellular RNA LV3
                    Up to 1x106 cultured cells in 200 μL PBS     50, 100, 200 μL 1:30
RNA Blood LV 4003 400 μL                         50, 100, 200 μL 1:30
RNA Blood LV 8003 800 μL                         100,
200 μL
1:30
RNA PAXgene LV3 PAXgene pellet in 400 μL PBS                         100,
200 μL
1:30
RNA PAXgene Half Tube LV3 Half PAXgene pellet in 200 μL PBS                         50, 
100 μL
1:30
RNA Tissue FF Standard LV3                       Up to 10 mg in 350 μL   50, 100, 200 μL 1:30

RNA Tissue FF High LV3

                      Up to 25 mg in 700 μL   100, 200 μL 1:30

RNA Tissue FFPE LV3

                        1x 5-10μm section 50,
100 μL
1:30
MagNA Pure 96 Cellular RNA Large Volume Kit (cat no. 05 467 535 001)

*BAL - Bronchoalveolar Lavage
**These times are approximate and should only be used as a guidance.

To get better results, Roche provides various External Lysis Buffers: MagNA Pure cfNA Buffer Set ( Cat. No. 07 794 398 001), MagNA Pure 96 External Lysis Buffer (Cat. No. 06 374 913 001), MagNA Pure 96 Bacterial Lysis Buffer (Cat. No. 06 374 921 001), and MagNA Pure 96 DNA Tissue Lysis Buffer (Cat. No. 06 640 702 001). Please refer to the Instructions for Use manual for more details.
 Target: Liquid handling
 Protocol name  Input sample type and sample volumes Elution volume Run time**
(h:mm)
 Whole  blood Plasma (citrate)  Plasma  (EDTA)  Serum   Swabs   BAL*  Sputum   CSF   Urine   Stool   Cultured cells Fresh-frozen tissue  FFPET 
Sample Transfer to the processing cartridge     N/A
Archiving Plate Transfer
For archiving purposes 
5 μL to 200 μL   N/A
PCR Setup with Mixing 5 μL to 200 μL
  N/A
PCR Setup without Mixing 5 μL to 200 μL
  N/A

 *BAL=Bronchoalveolar Lavage
**These times are approximate and should only be used as a guidance.

To get better results, Roche provides various External Lysis Buffers: MagNA Pure cfNA Buffer Set ( Cat. No. 07 794 398 001), MagNA Pure 96 External Lysis Buffer (Cat. No. 06 374 913 001), MagNA Pure 96 Bacterial Lysis Buffer (Cat. No. 06 374 921 001), and MagNA Pure 96 DNA Tissue Lysis Buffer (Cat. No. 06 640 702 001). Please refer to the Instructions for Use manual for more details.
Discover effortless nucleic acid isolation


Magnetism is used for automated nucleic acid isolation performed by the MagNA Pure 96 System from Roche. The high throughput MagNA Pure 96 Instrument performs automated nucleic acid purification from samples up to 1,000 µL, pipetted into a 96-well processing cartridge.

Magnetic glass particles (MGPs) are used to isolate RNA, DNA and total nucleic acids (tNA) from a variety of different sample materials. The MagNA Pure 96 System permits automated nucleic acid purification that matches the high speed, accuracy and reliability of LightCycler® Real-Time PCR Systems.
 

Key features of the MagNA Pure 96 System are:

 

Sample Number:

1 to 96 reactions per run

Sample volume:

  • 50 to 500 µL with 96 reactions per run
  • 1000 µL with 48 reactions per run

Maximum number of sample types within one run:

10 (whole blood, plasma, serum, urine, swabs, stool, sputum, BAL, CSF, bacterial cultures)

Run time for 96 reactions:

  • Approx. 50-60 minutes for 200 µL sample volumes
  • Approx. 80-90 minutes for 500 µL sample volumes

Reagent type:

Prefilled, ready-to-use

LIMS connectivity:

Yes

21CFR part 11 (subsection B) compatibility:

Yes

IVD compliance:

Instrument complies with the requirements of IVD directive 98/79/EC.

 

The MagNA Pure 96 System is currently on software version 3.0.
If you need assistance to update your system software, please contact your local Roche representative.