MagNA Pure 96 Instrument

High-throughput robotic workstation for fully automated purification of nucleic acids from up to 96 samples.

For in vitro diagnostic use.

Product No.	Pack Size
06541089001	1 instrument, control unit and accessories

To order these products, please contact your Roche order management team.

Keywords: Automated Isolation & Purification, MagNA Pure 96 Instrument, Instruments

The MagNA Pure 96 System purifies DNA, RNA, and viral nucleic acids from a wide range of starting materials using magnetic glass particle technology.

Preprogrammed software protocols are tailored to a diversity of sample requirements. Resulting nucleic acids are suitable for a broad range of downstream applications.

The MagNA Pure 96 System effortlessly processes up to 96 samples in less than one hour using barcoded, prefilled trays with ready-to-use reagents.

Experience seamless data management using intuitive features, such as the link to a laboratory information management system (LIMS).

Feature:	Benefit:
Speed and throughput Approximately 1 hour for processing a 96 sample run Multiple sample types can be processed in one run	Quick time-to-result for clinicians and patients
Convenience Only two kits needed to cover various diagnostic applications Few reagents and consumables needed Connectivity (LIMS and bidirectional file sharing)	Minimized error risk Low training effort & hands-on time
Robustness Insusceptible against tested substance interferences Very stable against cross contamination	Confidence & trust in your results
Performance Broad range of sample materials Excellent precision, purification efficiency and linear range Minimal lot-to-lot variation	One solution for various needs
Service and support Globally, approximately 3,500 employees at Roche Diagnostics are dedicated to service and support Roche is a leading supplier of IVD products. No other company has more experience in molecular diagnostics	Minimum downtime Cost effectiveness

The MagNA Pure 96 System is a high-throughput instrument for automated nucleic acid purification. Typical run duration is less than one hour for 96 standard volume samples. Applications are very flexible, due to a diversity of software purification protocols using barcoded, prefilled trays with ready-to-use reagents.

MagNA Pure 96 kits are designed profiting from our more than ten year expertise in magnetic glass particle technology. We have streamlined the purification process to a very small number of reagents, consumables and steps.

Optimized preprogrammed purification protocols ensure routine sample handling and high quality, reproducible nucleic acid yields. Choose the appropriate purification protocol for your sample material and target nucleic acid:

Target: Bacterial, fungal and viral NA
Protocol name	Input sample type and sample volumes													Elution volume	Run time** (h:mm)
Protocol name	Whole blood	Plasma (citrate)	Plasma (EDTA)	Serum	Swabs	BAL^*	Sputum	CSF	Urine	Stool	Cultured cells	Fresh-frozen tissue	FFPET	Elution volume	Run time** (h:mm)
Pathogen Universal 200¹	200 μL	200 μL	200 μL	200 μL	200 μL	200 μL	200 μL	200 μL	200 μL	200 μL				50, 100 μL	1:00
Viral NA Plasma SV¹			50, 100, 200 μL											50, 100 μL	1:00
Viral NA Plasma ext lys SV¹ Protocol optimized with external lysis reagent	100, 200 μL	100, 200 μL	100, 200 μL	100, 200 μL										50, 100 μL	1:00
Viral NA Universal SV¹	50, 100, 200 μL	50, 100, 200 μL		50, 100, 200 μL										50, 100 μL	1:00
Pathogen Universal 500²	500 μL	500 μL	500 μL	500μL	500 μL	500 μL	500 μL	500 μL	500 μL	500 μL				50, 100 μL	1:30
Pathogen Universal 1000²	1000 μL	1000 μL	1000 μL	1000 μL	1000 μL	1000 μL	1000 μL	1000 μL	1000 μL	1000 μL				50, 100 μL	1:40
Viral NA Plasma LV²			500 μL											50, 100 μL	1:10
Viral NA Plasma LV 1000²			1000 μL											50, 100 μL	1:20
Viral NA Plasma ext lys LV² Protocol optimized with external lysis reagent		500 μL	500 μL	500 μL										50, 100 μL	1:10
Viral NA Universal LV²		500 μL		500 μL										50, 100 μL	1:30
Viral NA Universal LV 1000²		1000 μL		1000 μL										50, 100 μL	1:30
¹MagNA Pure 96 DNA and Viral NA Small Volume Kit (cat. no. 06 543 588 001) ² MagNA Pure 96 DNA and Viral NA Large Volume Kit (cat. No. 06 374 891 001)								^BAL - Bronchoalveolar Lavage ^*These times are approximate and should only be used as a guidance.
To get better results, Roche provides various External Lysis Buffers: MagNA Pure cfNA Buffer Set ( Cat. No. 07 794 398 001), MagNA Pure 96 External Lysis Buffer (Cat. No. 06 374 913 001), MagNA Pure 96 Bacterial Lysis Buffer (Cat. No. 06 374 921 001), and MagNA Pure 96 DNA Tissue Lysis Buffer (Cat. No. 06 640 702 001). Please refer to the Instructions for Use manual for more details.

Target: Genomic DNA
Protocol name	Input sample type and sample volumes													Elution volume	Run time^** (h:mm)
Protocol name	Whole blood	Plasma (citrate)	Plasma (EDTA)	Serum	Swabs	BAL^*	Sputum	CSF	Urine	Stool	Cultured cells	Fresh-frozen tissue	FFPET	Elution volume	Run time^** (h:mm)
DNA Blood ds SV^{1, 4} Protocol optimized for double-stranded DNA and NGS	200 μL													50, 100 μL	1:00
DNA Blood SV¹	50, 100, 200 μL													50, 100 μL	1:00
DNA Blood ext lys SV¹ Protocol optimized with external lysis reagent	50, 100, 200 μL	50, 100, 200 μL	50, 100, 200 μL	50, 100, 200 μL										50, 100 μL	0:50
DNA Cells SV¹											Up to 5x10⁵ cultured cells in 200 μL PBS			100 μL	1:00
DNA Tissue SV¹											Up to 5mg in 200 μL			100, 200 μL	1:20
DNA FFPE SV¹													Lysate in 200 μL	50, 100 μL	1:00
DNA Blood LV²	500 μL													100, 200 μL	1:20
DNA Blood LV 1000²	1000 μL													100, 200 μL	1:30
DNA Blood ext lys LV² Protocol optimized with external lysis reagent	500 μL	500 μL	500 μL	500 μL										100, 200 μL	0:50
DNA Cells LV¹											Up to 1x10⁶ cultured cells in 200 μL PBS			100, 200 μL	1:20
DNA Tissue LV²											Up to 25 mg in 200 μL			200 μL	1:20
¹MagNA Pure 96 DNA and Viral NA Small Volume Kit (cat. no. 06 543 588 001)²MagNA Pure 96 DNA and Viral NA Large Volume Kit (cat. No. 06 374 891 001) ⁴ New Protocols								BAL - Bronchoalveolar Lavage *These times are approximate and should only be used as a guidance.
To get better results, Roche provides various External Lysis Buffers: MagNA Pure cfNA Buffer Set ( Cat. No. 07 794 398 001), MagNA Pure 96 External Lysis Buffer (Cat. No. 06 374 913 001), MagNA Pure 96 Bacterial Lysis Buffer (Cat. No. 06 374 921 001), and MagNA Pure 96 DNA Tissue Lysis Buffer (Cat. No. 06 640 702 001). Please refer to the Instructions for Use manual for more details.

Target: Cell-free NA
Protocol name	Input sample type and sample volumes													Elution volume	Run time** (h:mm)
Protocol name	Whole blood	Plasma (citrate)	Plasma (EDTA)	Serum	Swabs	BAL^*	Sputum	CSF	Urine	Stool	Cultured cells	Fresh-frozen tissue	FFPET	Elution volume	Run time** (h:mm)
cfNA ss 2000² Protocol optimized for single-stranded DNA			2000 μL											50, 100 μL	2:00
cfNA ss 4000² Protocol optimized for single-stranded DNA			4000 μL											50, 100 μL	2:20
cfNA ds 2000² Protocol optimized for double-stranded DNA			2000 μL											100, 200 μL	2:20
cfNA ds 4000² Protocol optimized for double-stranded DNA			4000 μL											100, 200 μL	2:50
²MagNA Pure 96 DNA and Viral NA Large Volume Kit (cat. No. 06 374 891 001)								BAL - Bronchoalveolar Lavage *These times are approximate and should only be used as a guidance.
To get better results, Roche provides various External Lysis Buffers: MagNA Pure cfNA Buffer Set ( Cat. No. 07 794 398 001), MagNA Pure 96 External Lysis Buffer (Cat. No. 06 374 913 001), MagNA Pure 96 Bacterial Lysis Buffer (Cat. No. 06 374 921 001), and MagNA Pure 96 DNA Tissue Lysis Buffer (Cat. No. 06 640 702 001). Please refer to the Instructions for Use manual for more details.

Target: Total RNA
Protocol name	Input sample type and sample volumes													Elution volume	Run time** (h:mm)
Protocol name	Whole blood	Plasma (citrate)	Plasma (EDTA)	Serum	Swabs	BAL^*	Sputum	CSF	Urine	Stool	Cultured cells	Fresh-frozen tissue	FFPET	Elution volume	Run time** (h:mm)
Cellular RNA LV³											Up to 1x10⁶ cultured cells in 200 μL PBS			50, 100, 200 μL	1:30
RNA Blood LV 400³	400 μL													50, 100, 200 μL	1:30
RNA Blood LV 800³	800 μL													100, 200 μL	1:30
RNA PAXgene LV³	PAXgene pellet in 400 μL PBS													100, 200 μL	1:30
RNA PAXgene Half Tube LV³	Half PAXgene pellet in 200 μL PBS													50, 100 μL	1:30
RNA Tissue FF Standard LV³												Up to 10 mg in 350 μL		50, 100, 200 μL	1:30
RNA Tissue FF High LV³												Up to 25 mg in 700 μL		100, 200 μL	1:30
RNA Tissue FFPE LV³													1x 5-10μm section	50, 100 μL	1:30
³MagNA Pure 96 Cellular RNA Large Volume Kit (cat no. 05 467 535 001)								BAL - Bronchoalveolar Lavage *These times are approximate and should only be used as a guidance.
To get better results, Roche provides various External Lysis Buffers: MagNA Pure cfNA Buffer Set ( Cat. No. 07 794 398 001), MagNA Pure 96 External Lysis Buffer (Cat. No. 06 374 913 001), MagNA Pure 96 Bacterial Lysis Buffer (Cat. No. 06 374 921 001), and MagNA Pure 96 DNA Tissue Lysis Buffer (Cat. No. 06 640 702 001). Please refer to the Instructions for Use manual for more details.

Target: Liquid handling
Protocol name	Input sample type and sample volumes													Elution volume	Run time** (h:mm)
Protocol name	Whole blood	Plasma (citrate)	Plasma (EDTA)	Serum	Swabs	BAL*	Sputum	CSF	Urine	Stool	Cultured cells	Fresh-frozen tissue	FFPET	Elution volume	Run time** (h:mm)
Sample Transfer to the processing cartridge															N/A
Archiving Plate Transfer For archiving purposes	5 μL to 200 μL														N/A
PCR Setup with Mixing	5 μL to 200 μL														N/A
PCR Setup without Mixing	5 μL to 200 μL														N/A
^BAL=Bronchoalveolar Lavage *These times are approximate and should only be used as a guidance.
To get better results, Roche provides various External Lysis Buffers: MagNA Pure cfNA Buffer Set ( Cat. No. 07 794 398 001), MagNA Pure 96 External Lysis Buffer (Cat. No. 06 374 913 001), MagNA Pure 96 Bacterial Lysis Buffer (Cat. No. 06 374 921 001), and MagNA Pure 96 DNA Tissue Lysis Buffer (Cat. No. 06 640 702 001). Please refer to the Instructions for Use manual for more details.

Discover effortless nucleic acid isolation

Magnetism is used for automated nucleic acid isolation performed by the MagNA Pure 96 System from Roche. The high throughput MagNA Pure 96 Instrument performs automated nucleic acid purification from samples up to 1,000 µL, pipetted into a 96-well processing cartridge.

Magnetic glass particles (MGPs) are used to isolate RNA, DNA and total nucleic acids (tNA) from a variety of different sample materials. The MagNA Pure 96 System permits automated nucleic acid purification that matches the high speed, accuracy and reliability of LightCycler^® Real-Time PCR Systems.

Key features of the MagNA Pure 96 System are:

Sample Number:	1 to 96 reactions per run
Sample volume:	50 to 500 µL with 96 reactions per run 1000 µL with 48 reactions per run
Maximum number of sample types within one run:	10 (whole blood, plasma, serum, urine, swabs, stool, sputum, BAL, CSF, bacterial cultures)
Run time for 96 reactions:	Approx. 50-60 minutes for 200 µL sample volumes Approx. 80-90 minutes for 500 µL sample volumes
Reagent type:	Prefilled, ready-to-use
LIMS connectivity:	Yes
21CFR part 11 (subsection B) compatibility:	Yes
IVD compliance:	Instrument complies with the requirements of IVD directive 98/79/EC.

The MagNA Pure 96 System is currently on software version 3.0.
If you need assistance to update your system software, please contact your local Roche representative.

Associated Products

FLOW Primary Sample Handling Instrument Read more

MagNA Pure 96 DNA and Viral NA Small Volume Kit Read more

MagNA Pure Tip 1000 µL Read more

MagNA Pure 96 Processing Cartridge Read more

DNA Process Control Kit Read more

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MagNA Pure 96 Instrument

Feature:

Benefit:

Discover effortless nucleic acid isolation

Key features of the MagNA Pure 96 System are:

Associated Products