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"Code": "Storage Conditions (Product)", "Name": "Storage Conditions (Product)" }, { "Language": "en", "Value": "Easy-to-use reaction mix for reverse transcription", "Country": "XG", "Code": "Product Characteristics", "Name": "Product Characteristics" }, { "Language": "en", "Value": "
StepTime [min]
RT reaction setup time30
RT reaction30
PCR reaction setup time30
PCR reaction120
Total Time3 hours 30 minutes
", "Country": "XG", "Code": "Assay Time", "Name": "Assay Time" }, { "Language": "en", "Value": "
Vial / Bottle Cap Label Function / Description Catalog Number Content
1 blue EvoScript Reverse Transcriptase,
Reverse Transcriptase
Contains Protector RNase Inhibitor and
Enzyme Blend, 10x conc.
07 912 315 001 1 vial,
100 μl
07 912 323 001 4 vials,
100 μl each
2 red EvoScript Reverse Transcriptase,
Reaction Buffer
Contains dNTPs, Mg(OAc)2,
​​​​​​​and salts, 5x conc.
07 912 315 001 1 vial,
200 μl
07 912 323 001 4 vials,
200 μl each
3 colorless EvoScript Reverse Transcriptase,
Water, PCR Grade
To adjust the final reaction volume. 07 912 315 001 1 vial,
1 ml
07 912 323 001 4 vials,
1 ml each
", "Country": "XG", "Code": "Content", "Name": "Content" }, { "Language": "en", "Value": "The EvoScript Reverse Transcriptase is designed for highly sensitive and convenient cDNA synthesis in two-step real-time RT-qPCR and is compatible with the LightCycler® 480 System, LightCycler® 96 System, or other real-time PCR instruments. The 2-vial composition is ideally suited for reverse transcription, requiring only the addition of gene-specific primers and target RNA.", "Country": "XG", "Code": "Applications", "Name": "Applications" }, { "Language": "en", "Value": "The EvoScript Reverse Transcriptase provides a convenient solution for time-saving cDNA synthesis for use in two-step, real-time RT-PCR. All reagents needed for cDNA synthesis, except gene-specific RT primer, but including nucleotides, buffers, and enzyme blend are supplied in only two vials, minimizing pipetting efforts. The enzyme blend included in this kit has a broad temperature range and is suitable for high temperature reverse transcription. The product is tested for purified cellular RNA, mRNA, and cell lysates generated using the RealTime ready Cell Lysis Buffer* which further accelerates fast real-time PCR directly on cell lysates. If large amounts of cDNA are required, the reaction can be upscaled without influencing the product yield.
The EvoScript Reverse Transcriptase enables reliable cDNA synthesis over a wide dynamic range even for GC-rich templates, and is ideal for high-throughput quantitative RT-PCR analysis.", "Country": "XG", "Code": "Principle", "Name": "Principle" } ] } } ] }

EvoScript Reverse Transcriptase

Easy-to-use reaction mix for reverse transcription

EvoScript Reverse Transcriptase

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