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Characteristics", "Name": "Product Characteristics" }, { "Language": "en", "Value": "

Step	Time [min]
RT reaction setup time	30
RT reaction	35
PCR reaction setup time	30
PCR reaction	60
Total Time	2 hours 35 minutes

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Vial / Bottle	Cap	Label	Function / Description	Catalog Number	Content
1	blue	EvoScript Universal cDNA Master, Enzyme Mix	Contains Enzyme Blend for reverse transcription and Protector RNase Inhibitor, 10x conc.	07 912 374 001	1 vial, 100 μl
07 912 439 001	2 vials, 100 μl each
07 912 455 001	4 vials, 100 μl each
2	red	EvoScript Universal cDNA Master, Reaction Buffer	Contains random primers, anchored oligo(dT)18, dNTP, and Mg(OAc)2, 5x conc.	07 912 374 001	1 vial, 200 μl
07 912 439 001	2 vials, 200 μl each
07 912 455 001	4 vials, 200 μl each
3	colorless	EvoScript Universal cDNA Master, Water, PCR Grade	To adjust the final reaction volume.	07 912 374 001	1 vial, 1 ml
07 912 439 001	2 vials, 1 ml each
07 912 455 001	4 vials, 1 ml each

", "Country": "XG", "Code": "Content", "Name": "Content" }, { "Language": "en", "Value": "The EvoScript Universal cDNA Master provides a convenient solution for time-saving cDNA Synthesis for use in two-step, real-time RT-qPCR. All reagents needed for cDNA synthesis, including primers, nucleotides, buffers, and enzymes are supplied in only two vials, minimizing pipetting efforts. The enzyme blend included in this kit has a broad temperature range and is suitable for high temperature reverse transcription. The amount of random primer and oligo(dT)₁₈ primer included in the EvoScript Universal cDNA Master Reaction Buffer enables high cDNA yields from all regions of the RNA template. The product is tested for purified cellular RNA, mRNA, and cell lysates generated using the RealTime ready Cell Lysis Buffer* which further accelerates fast real-time PCR directly on cell lysates. If large amounts of cDNA are required, the reaction can be upscaled without influencing the product yield.
The EvoScript Universal cDNA Master enables reliable cDNA synthesis over a wide dynamic range even for GC-rich templates, and is ideal for high-throughput quantitative RT-PCR analysis. The mix contains an optimized concentration of Mg(OAc)₂, eliminating the need for additional adjustments.", "Country": "XG", "Code": "Principle", "Name": "Principle" } ] } } ] }