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RealTime ready Cell Lysis Buffer

Easy-to-use reagent for cell lysis, prior to real-time RT-PCR applications.

For life science research only. Not for use in diagnostic procedures.

Product No. Pack Size
07248431001 5000 reactions

To order these products, please contact your Roche order management team.

RealTime ready Cell Lysis Buffer is an easy-to-use reagent for the lysis of cultured cells prior to reverse transcription.

  • Increase convenience:
    Purify RNA using a simple one-step cell lysis procedure instead of cumbersome multi-step protocols.
    Easily store and reduce handling steps for large scale projects with this bulk pack size.
  • Speed time to result:
    Lyse samples in just 5 minutes at room temperature without further steps, unlike other lysis methods.
  • Benefit from broad applicability:
    Scale up from 3 to 30,000 cultured cells for real-time PCR applications in 96- to 1536-well formats, with or without automation.
  • Minimize RNA Loss
    Minimize RNA loss by utilizing the whole cell lysate as a starting material for RT-qPCR, when compared to column-based purification methods.
  • Minimize waste 
    Limit the waste materials to be managed by the lab with a bulk format of 5000 reactions in one bottle

Contents

Ready-to-use Cell Lysis Buffer (200 ml)

The RealTime ready Cell Lysis Buffer is designed for the lysis of cultured cells. Cell lysates can then be directly used for RT-qPCR or cDNA synthesis followed by a qPCR step. The quality of RNA isolated is suitable for relative quantification of mRNA by qPCR. The RealTime ready Cell Lysis Buffer is designed for rapid, high-performance gene expression studies using cultured cells in RT-qPCR or qPCR applications. The buffer uses an advanced one-step protocol for lysing 3 to 30,000 cells within 5 minutes at room temperature. The resulting lysate can be used directly for downstream real-time PCR applications, eliminating the time-consuming RNA purification step and ensuring minimal loss of RNA. With this large pack size and easy-to-use protocol, the RealTime ready Cell Lysis Buffer can be easily adapted to high throughput experiments while minimizing waste materials. In addition, this buffer easily integrates into automated cell-based gene expression workflows.


The RealTime ready Cell Lysis Buffer is optimal when used with the following products for downstream cDNA synthesis and real-time PCR analysis: Protector RNase Inhibitor, Transcriptor cDNA Synthesis Kits, LightCycler® Master Mixes, Universal ProbeLibrary probes, RealTime ready assays, and LightCycler® Instruments.

Quality


The RealTime ready Cell Lysis Buffer is tested to meet specifications of RT-qPCR.