{ "ProductData": { "ID": "3.5.9.5.3.5", "ProductType": "Others", "BrandName": "LightCycler^® EvoScript RNA SYBR^® Green I Master", "ProductNameAddition": "", "RegulatoryDisclaimer1": "For general laboratory use.", "DisclaimerGroup1": "GLU", "RegulatoryDisclaimer2": null, "DisclaimerGroup2": null, "RegulatoryDisclaimer3": null, "SampleType": [ "RNA" ], "LicenseDisclaimers": [ { "Identifier": "1280837236338", "LongText": "SYBR Green I is manufactured by Molecular Probes, Inc., and is provided under license from Molecular Probes, Inc., for direct research use for PCR, where the dye is present during the PCR.", "Order": "67" } ], "RelatedLinks": "", "Clone": "", "ControlTissue": [ "" ], "ISOtypes": "", "Species": [ "" ], "StainLocalization": [ "" ], "ProductNameGlobal": "LightCycler EvoScript RNA SYBR Green I Master" }, "ProductImageDetails": { "ImagePath": "https://pim-media.roche.com/Images/Article_07800177001_im_en.png", "ImageType": "Image main" }, "Product2Taxonomy": { "Product2TaxonomyReferences": [ { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeID": "06-0020", "StructureNodeStatus": "Active", "NodeName": "LightCycler 96 and 480 System Kits" }, { "StructureSystemIdentifier": "OWP_Product_Types", "StructureSystemName": "Product Types", "NodeID": "20-000-00", "StructureNodeStatus": "Active", "NodeName": "Assays Reagents and Strips" }, { "StructureSystemIdentifier": "OWP_Family", "StructureSystemName": "Product Families", "NodeID": "230", "StructureNodeStatus": "Active", "NodeName": "LightCycler" }, { "StructureSystemIdentifier": "OWP_Techniques", "StructureSystemName": "Techniques", "NodeID": "210-00", "StructureNodeStatus": "Active", "NodeName": "PCR/qPCR" }, { "StructureSystemIdentifier": "Lab_Type", "StructureSystemName": "Lab Types", "NodeID": "150-00", "StructureNodeStatus": "Active", "NodeName": "Research Lab" }, { "StructureSystemIdentifier": "Product_Solutions", "StructureSystemName": "Product Solutions", "NodeID": "100", "StructureNodeStatus": "Active", "NodeName": "Molecular" }, { "StructureSystemIdentifier": "Applications", "StructureSystemName": "Applications", "NodeID": "08-00-00", "StructureNodeStatus": "Active", "NodeName": "Gene Expression Analysis" }, { "StructureSystemIdentifier": "Lab_Type", "StructureSystemName": "Lab Types", "NodeID": "170-00", "StructureNodeStatus": "Inactive", "NodeName": "" }, { "StructureSystemIdentifier": "Health_Topics", "StructureSystemName": "Health Topics", "NodeID": "99-00-00", "StructureNodeStatus": "Inactive", "NodeName": "" }, { "StructureSystemIdentifier": "Pathogens", "StructureSystemName": "Pathogens", "NodeID": "99-00-00", "StructureNodeStatus": "Inactive", "NodeName": "" }, { "StructureSystemIdentifier": "Disease_Areas", "StructureSystemName": "Disease Areas", "NodeID": "99-00-00", "StructureNodeStatus": "Inactive", "NodeName": "" } ] }, "Product2Materials": { "P2MaterialReferences": [ { "MaterialNum": "07800177001", "MaterialDescription": "LC EvoScript RNA SYBR Green Master 1000", "RegisteredProductName": "LightCycler EvoScript RNA SYBR Green I Master", "GTIN": "04038377029580", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "1 kit", "PackSizeDescPIM360": "1,000 reactions of 20 µl final volume each", "MaterialAnnotation": "", "ReadyForUse": "", "OrderInformation": "" }, { "MaterialNum": "07800134001", "MaterialDescription": "LC EvoScript RNA SYBR Green Master (200)", "RegisteredProductName": "LightCycler EvoScript RNA SYBR Green I Master", "GTIN": "04038377029573", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "1 kit", "PackSizeDescPIM360": "200 reactions of 20 µl final volume each", "MaterialAnnotation": "", "ReadyForUse": "", "OrderInformation": "" } ] }, "Product2Products": { "Product2ProductReference": [ ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Name": "Storage Conditions (Product)", "Value": "Store the kit at −15 to −25°C.", "Language": "en", "Country": "XG", "Code": "Storage Conditions (Product)" }, { "Name": "Mg2+ Concentration", "Value": "The master mix is optimized with a fixed concentration of Mg(OAc)₂, which works with nearly all primer combinations. There is no need for adjustment.", "Language": "en", "Country": "XG", "Code": "Mg2+ Concentration" }, { "Name": "Typical run time", "Value": "When using the recommended thermal profile on the LightCycler^® 480 System or LightCycler^® 96 System, the LightCycler^® EvoScript RNA SYBR^® Green I Master run time is approximately 105 minutes. The RT-qPCR thermal profile includes a 15 minute reverse transcription step and 45 cycles of PCR.", "Language": "en", "Country": "XG", "Code": "Typical run time" }, { "Name": "Applications", "Value": "The LightCycler^® EvoScript RNA SYBR^® Green I Master is designed for sensitive, high-specificity, high-precision one-step RT-qPCR especially for gene expression assays.
The 1-vial composition is ideally suited for easy reaction assembly, requiring only the addition of primers and target RNA. The proprietary reaction buffer allows a convenient hot start RT-qPCR. In addition, the enzymes for reverse transcription and for PCR feature hot start for premium specificity⁽¹⁾. The master mix is optimized for SYBR^® Green I applications and does not require optimization with Mg(OAc)₂.

1. The reverse transcription activity will be suppressed at temperatures significantly below 60°C.

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Vial / Bottle	Cap	Label	Function / Description	Catalog Number	Content
1	blue	LightCycler® EvoScript RNA SYBR® Green I Master, Master, 5x conc.	Contains enzymes for reverse transcription and PCR,RT-qPCR Reaction Buffer, dATP, dCTP, dGTP, and dUTP, Mg(OAc)2, SYBR® Green I dye, and proprietary additives.	07 800 134 001	2 vials, 440 μl each
07 800 177 001	10 vials, 440 μl each
2	colorless	LightCycler® EvoScript RNA SYBR® Green I Master, Water, PCR Grade	To adjust the final reaction volume.	07 800 134 001	3 vials, 1 ml each
07 800 177 001	2 vials, 7.25 ml each

", "Language": "en", "Country": "XG", "Code": "Content" }, { "Name": "Principle", "Value": "The LightCycler^® EvoScript RNA SYBR^® Green I Master consists of 2 different
vials:

• Vial 1: LightCycler^® EvoScript RNA SYBR^® Green I Master, 5x conc.
• Vial 2: Water, PCR Grade

The kit also provides sufficient numbers of vials containing Water, PCR Grade to ensure that fresh (unopened) vials can be used. This minimizes the risk of contamination of RT-qPCR reaction mixes with RNases and other substances.
The resulting RT-qPCR reaction mix also has a hot start Taq DNA Polymerase, nucleotides, and additives ensuring a hot start amplification system with high specificity. The mix contains an optimized concentration of Mg(OAc)₂, eliminating the need for additional adjustments. For greater convenience, the RT-qPCR mixture can be used for both RNA and/or DNA templates in parallel.", "Language": "en", "Country": "XG", "Code": "Principle" }, { "Name": "Product Description", "Value": "The LightCycler^® EvoScript RNA SYBR^® Green I Master as a 1-vial composition, is designed for sensitive, high-specificity, high-precision one-step RT-qPCR especially for gene expression assays.", "Language": "en", "Country": "XG", "Code": "Product Description" }, { "Name": "Assay Time", "Value": "105 minutes (includes a 15 minute reverse transcription step and 45 cycles of PCR)", "Language": "en", "Country": "XG", "Code": "Assay Time" }, { "Name": "Product Purpose", "Value": "The LightCycler^® EvoScript RNA SYBR^® Green I Master as a 1-vial composition, is designed for sensitive, high-specificity, high-precision one-step RT-qPCR especially for gene expression assays.", "Language": "en", "Country": "XG", "Code": "Product Purpose" } ] } } ] }