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  1. The reverse transcription activity will be suppressed at temperatures significantly below 60°C.
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Vial / BottleCapLabelFunction / DescriptionCatalog NumberContent
1blueLightCycler® EvoScript RNA SYBR® Green I Master,
Master, 5x conc.
Contains enzymes for reverse transcription
and PCR,RT-qPCR Reaction Buffer, dATP,
dCTP, dGTP, and dUTP,
Mg(OAc)2, SYBR® Green I dye,
and proprietary additives.
07 800 134 0012 vials,
440 μl each
07 800 177 00110 vials,
440 μl each
2colorlessLightCycler® EvoScript RNA SYBR® Green I Master,
Water, PCR Grade
To adjust the final reaction volume.07 800 134 0013 vials,
1 ml each
07 800 177 0012 vials,
7.25 ml each
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vials:
  • Vial 1: LightCycler® EvoScript RNA SYBR® Green I Master, 5x conc.
  • Vial 2: Water, PCR Grade
The kit also provides sufficient numbers of vials containing Water, PCR Grade to ensure that fresh (unopened) vials can be used. This minimizes the risk of contamination of RT-qPCR reaction mixes with RNases and other substances.
The resulting RT-qPCR reaction mix also has a hot start Taq DNA Polymerase, nucleotides, and additives ensuring a hot start amplification system with high specificity. The mix contains an optimized concentration of Mg(OAc)2, eliminating the need for additional adjustments. For greater convenience, the RT-qPCR mixture can be used for both RNA and/or DNA templates in parallel.", "Language": "en", "Country": "XG", "Code": "Principle" }, { "Name": "Product Description", "Value": "The LightCycler® EvoScript RNA SYBR® Green I Master as a 1-vial composition, is designed for sensitive, high-specificity, high-precision one-step RT-qPCR especially for gene expression assays.", "Language": "en", "Country": "XG", "Code": "Product Description" }, { "Name": "Assay Time", "Value": "105 minutes (includes a 15 minute reverse transcription step and 45 cycles of PCR)", "Language": "en", "Country": "XG", "Code": "Assay Time" }, { "Name": "Product Purpose", "Value": "The LightCycler® EvoScript RNA SYBR® Green I Master as a 1-vial composition, is designed for sensitive, high-specificity, high-precision one-step RT-qPCR especially for gene expression assays.", "Language": "en", "Country": "XG", "Code": "Product Purpose" } ] } } ] }

LightCycler® EvoScript RNA SYBR® Green I Master

GPR For general laboratory use.
LightCycler<sup>®</sup> EvoScript RNA SYBR<sup>®</sup> Green I Master

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