{ "ProductData": { "RelatedLinks": "", "RegulatoryDisclaimer1": "For general laboratory use.", "RegulatoryDisclaimer2": "", "StainLocalization": [ "" ], "ProductType": "Others", "DisclaimerGroup2": null, "ProductNameGlobal": "LightCycler EvoScript RNA SYBR Green I Master", "DisclaimerGroup1": "GPR", "ControlTissue": [ "" ], "BrandName": "LightCycler® EvoScript RNA SYBR® Green I Master", "LicenseDisclaimers": [ { "Order": "67", "Identifier": "1280837236338", "LongText": "SYBR Green I is manufactured by Molecular Probes, Inc., and is provided under license from Molecular Probes, Inc., for direct research use for PCR, where the dye is present during the PCR." } ], "Isotypes": "", "RegulatoryDisclaimer3": "", "ID": "3.5.9.5.3.5", "ProductNameAddition": "Easy-to-use reaction mix for one-step RT-qPCR using the LightCycler® 480 or the LightCycler® 96 Real-Time PCR Systems", "Species": [ "" ], "Clone": "" }, "ProductImageDetails": { "ImagePath": "https://pim-media.roche.com/Images/Article_07800177001_im_en.png", "ImageType": "Image main" }, "Product2Taxomony": { "Product2TaxomonyReferences": [ { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeName": "LightCycler® 96 and 480 System Kits", "NodeID": "06-0020" }, { "StructureSystemIdentifier": "OWP_Product_Types", "StructureSystemName": "Product Types", "NodeName": "Assays Reagents and Strips", "NodeID": "20-000-00" }, { "StructureSystemIdentifier": "OWP_Family", "StructureSystemName": "Product Families", "NodeName": "LightCycler", "NodeID": "230" }, { "StructureSystemIdentifier": "OWP_Techniques", "StructureSystemName": "Techniques", "NodeName": "PCR/qPCR", "NodeID": "210-00" }, { "StructureSystemIdentifier": "Lab_Type", "StructureSystemName": "Lab Types", "NodeName": "Research Lab", "NodeID": "150-00" }, { "StructureSystemIdentifier": "Product_Solutions", "StructureSystemName": "Product Solutions", "NodeName": "Molecular", "NodeID": "100" }, { "StructureSystemIdentifier": "Applications", "StructureSystemName": "Applications", "NodeName": "Gene Expression Analysis", "NodeID": "08-00-00" }, { "StructureSystemIdentifier": "Lab_Type", "StructureSystemName": "Lab Types", "NodeName": "LifeScience", "NodeID": "170-00" }, { "StructureSystemIdentifier": "Health_Topics", "StructureSystemName": "Health Topics", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Pathogens", "StructureSystemName": "Pathogens", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Disease_Areas", "StructureSystemName": "Disease Areas", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" } ] }, "Product2Taxonomy": { "Product2TaxonomyReferences": [ { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeName": "LightCycler® 96 and 480 System Kits", "NodeID": "06-0020" }, { "StructureSystemIdentifier": "OWP_Product_Types", "StructureSystemName": "Product Types", "NodeName": "Assays Reagents and Strips", "NodeID": "20-000-00" }, { "StructureSystemIdentifier": "OWP_Family", "StructureSystemName": "Product Families", "NodeName": "LightCycler", "NodeID": "230" }, { "StructureSystemIdentifier": "OWP_Techniques", "StructureSystemName": "Techniques", "NodeName": "PCR/qPCR", "NodeID": "210-00" }, { "StructureSystemIdentifier": "Lab_Type", "StructureSystemName": "Lab Types", "NodeName": "Research Lab", "NodeID": "150-00" }, { "StructureSystemIdentifier": "Product_Solutions", "StructureSystemName": "Product Solutions", "NodeName": "Molecular", "NodeID": "100" }, { "StructureSystemIdentifier": "Applications", "StructureSystemName": "Applications", "NodeName": "Gene Expression Analysis", "NodeID": "08-00-00" }, { "StructureSystemIdentifier": "Lab_Type", "StructureSystemName": "Lab Types", "NodeName": "LifeScience", "NodeID": "170-00" }, { "StructureSystemIdentifier": "Health_Topics", "StructureSystemName": "Health Topics", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Pathogens", "StructureSystemName": "Pathogens", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Disease_Areas", "StructureSystemName": "Disease Areas", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" } ] }, "Product2Materials": { "P2MaterialReferences": [ { "MaterialNum": "07800177001", "MaterialDescription": "LC EvoScript RNA SYBR Green Master 1000", "RegisteredProductName": "LightCycler EvoScript RNA SYBR Green I Master", "GTIN": "04038377029580", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "1 kit", "PackSizeDescPIM360": "1,000 reactions of 20 µl final volume each", "MaterialAnnotation": "", "ReadyForUse": "", "OrderInformation": "" }, { "MaterialNum": "07800134001", "MaterialDescription": "LC EvoScript RNA SYBR Green Master (200)", "RegisteredProductName": "LightCycler EvoScript RNA SYBR Green I Master", "GTIN": "04038377029573", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "1 kit", "PackSizeDescPIM360": "200 reactions of 20 µl final volume each", "MaterialAnnotation": "", "ReadyForUse": "", "OrderInformation": "" } ] }, "Product2Products": { "Product2ProductReference": [ { "ProductID": "INS_445", "BrandName": "LightCycler® 480 Instrument II", "ProductNameAddition": "", "ReferenceType": "Instrument", "Classification": [ { "IdentifierofStructureSystem": "1", "NameofStructureSystem": "GPCH", "StructureNodeID": "417", "StructureGroupPath": "Life Science->RLS qPCR & NAP Systems->LightCycler 480", "StructureGroupName": "LightCycler 480" }, { "IdentifierofStructureSystem": "OWP_Family", "NameofStructureSystem": "Product Families", "StructureNodeID": "230", "StructureGroupPath": "LightCycler", "StructureGroupName": "LightCycler" }, { "IdentifierofStructureSystem": "OWP_Product_Types", "NameofStructureSystem": "Product Types", "StructureNodeID": "10-000-00", "StructureGroupPath": "Analyzer Instruments and Systems", "StructureGroupName": "Analyzer Instruments and Systems" }, { "IdentifierofStructureSystem": "OWP_Techniques", "NameofStructureSystem": "Techniques", "StructureNodeID": "210-00", "StructureGroupPath": "PCR/qPCR", "StructureGroupName": "PCR/qPCR" }, { "IdentifierofStructureSystem": "OWP_Techniques", "NameofStructureSystem": "Techniques", "StructureNodeID": "300-00", "StructureGroupPath": "RT-PCR/qRT-PCR", "StructureGroupName": "RT-PCR/qRT-PCR" }, { "IdentifierofStructureSystem": "Lab_Type", "NameofStructureSystem": "Lab Types", "StructureNodeID": "170-00", "StructureGroupPath": "LifeScience", "StructureGroupName": "LifeScience" }, { "IdentifierofStructureSystem": "Lab_Type", "NameofStructureSystem": "Lab Types", "StructureNodeID": "160-00", "StructureGroupPath": "Sequencing", "StructureGroupName": "Sequencing" }, { "IdentifierofStructureSystem": "Applications", "NameofStructureSystem": "Applications", "StructureNodeID": "99-00-00", "StructureGroupPath": "NOT APPLICABLE", "StructureGroupName": "NOT APPLICABLE" }, { "IdentifierofStructureSystem": "Disease_Areas", "NameofStructureSystem": "Disease Areas", "StructureNodeID": "99-00-00", "StructureGroupPath": "NOT APPLICABLE", "StructureGroupName": "NOT APPLICABLE" }, { "IdentifierofStructureSystem": "Pathogens", "NameofStructureSystem": "Pathogens", "StructureNodeID": "99-00-00", "StructureGroupPath": "NOT APPLICABLE", "StructureGroupName": "NOT APPLICABLE" }, { "IdentifierofStructureSystem": "Health_Topics", "NameofStructureSystem": "Health Topics", "StructureNodeID": "99-00-00", "StructureGroupPath": "NOT APPLICABLE", "StructureGroupName": "NOT APPLICABLE" }, { "IdentifierofStructureSystem": "Product_Solutions", "NameofStructureSystem": "Product Solutions", "StructureNodeID": "055", "StructureGroupPath": "Laboratory developed testing", "StructureGroupName": "Laboratory developed testing" }, { "IdentifierofStructureSystem": "Product_Solutions", "NameofStructureSystem": "Product Solutions", "StructureNodeID": "100", "StructureGroupPath": "Molecular", "StructureGroupName": "Molecular" }, { "IdentifierofStructureSystem": "Product_Solutions", "NameofStructureSystem": "Product Solutions", "StructureNodeID": "530", "StructureGroupPath": "Sequencing", "StructureGroupName": "Sequencing" } ] }, { "ProductID": "INS_2691", "BrandName": "LightCycler® 96 Instrument", "ProductNameAddition": "", "ReferenceType": "Instrument", "Classification": [ { "IdentifierofStructureSystem": "1", "NameofStructureSystem": "GPCH", "StructureNodeID": "6274", "StructureGroupPath": "Life Science->RLS qPCR & NAP Systems->LightCycler 96", "StructureGroupName": "LightCycler 96" }, { "IdentifierofStructureSystem": "OWP_Family", "NameofStructureSystem": "Product Families", "StructureNodeID": "230", "StructureGroupPath": "LightCycler", "StructureGroupName": "LightCycler" }, { "IdentifierofStructureSystem": "OWP_Product_Types", "NameofStructureSystem": "Product Types", "StructureNodeID": "10-000-00", "StructureGroupPath": "Analyzer Instruments and Systems", "StructureGroupName": "Analyzer Instruments and Systems" }, { "IdentifierofStructureSystem": "OWP_Techniques", "NameofStructureSystem": "Techniques", "StructureNodeID": "300-00", "StructureGroupPath": "RT-PCR/qRT-PCR", "StructureGroupName": "RT-PCR/qRT-PCR" }, { "IdentifierofStructureSystem": "OWP_Techniques", "NameofStructureSystem": "Techniques", "StructureNodeID": "210-00", "StructureGroupPath": "PCR/qPCR", "StructureGroupName": "PCR/qPCR" }, { "IdentifierofStructureSystem": "Product_Solutions", "NameofStructureSystem": "Product Solutions", "StructureNodeID": "530", "StructureGroupPath": "Sequencing", "StructureGroupName": "Sequencing" }, { "IdentifierofStructureSystem": "Lab_Type", "NameofStructureSystem": "Lab Types", "StructureNodeID": "170-00", "StructureGroupPath": "LifeScience", "StructureGroupName": "LifeScience" }, { "IdentifierofStructureSystem": "Product_Solutions", "NameofStructureSystem": "Product Solutions", "StructureNodeID": "055", "StructureGroupPath": "Laboratory developed testing", "StructureGroupName": "Laboratory developed testing" }, { "IdentifierofStructureSystem": "Applications", "NameofStructureSystem": "Applications", "StructureNodeID": "99-00-00", "StructureGroupPath": "NOT APPLICABLE", "StructureGroupName": "NOT APPLICABLE" }, { "IdentifierofStructureSystem": "Disease_Areas", "NameofStructureSystem": "Disease Areas", "StructureNodeID": "99-00-00", "StructureGroupPath": "NOT APPLICABLE", "StructureGroupName": "NOT APPLICABLE" }, { "IdentifierofStructureSystem": "Pathogens", "NameofStructureSystem": "Pathogens", "StructureNodeID": "99-00-00", "StructureGroupPath": "NOT APPLICABLE", "StructureGroupName": "NOT APPLICABLE" }, { "IdentifierofStructureSystem": "Health_Topics", "NameofStructureSystem": "Health Topics", "StructureNodeID": "99-00-00", "StructureGroupPath": "NOT APPLICABLE", "StructureGroupName": "NOT APPLICABLE" }, { "IdentifierofStructureSystem": "Product_Solutions", "NameofStructureSystem": "Product Solutions", "StructureNodeID": "100", "StructureGroupPath": "Molecular", "StructureGroupName": "Molecular" } ] } ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Language": "en", "Value": "Store the kit at −15 to −25°C.", "Country": "XG", "Code": "Storage Conditions (Product)", "Name": "Storage Conditions (Product)" }, { "Language": "en", "Value": "The master mix is optimized with a fixed concentration of Mg(OAc)2, which works with nearly all primer combinations. There is no need for adjustment.", "Country": "XG", "Code": "Mg2+ Concentration", "Name": "Mg2+ Concentration" }, { "Language": "en", "Value": "When using the recommended thermal profile on the LightCycler® 480 System or LightCycler® 96 System, the LightCycler® EvoScript RNA SYBR® Green I Master run time is approximately 105 minutes. The RT-qPCR thermal profile includes a 15 minute reverse transcription step and 45 cycles of PCR.", "Country": "XG", "Code": "Typical run time", "Name": "Typical run time" }, { "Language": "en", "Value": "The LightCycler® EvoScript RNA SYBR® Green I Master is designed for sensitive, high-specificity, high-precision one-step RT-qPCR especially for gene expression assays.
The 1-vial composition is ideally suited for easy reaction assembly, requiring only the addition of primers and target RNA. The proprietary reaction buffer allows a convenient hot start RT-qPCR. In addition, the enzymes for reverse transcription and for PCR feature hot start for premium specificity(1). The master mix is optimized for SYBR® Green I applications and does not require optimization with Mg(OAc)2.
  1. The reverse transcription activity will be suppressed at temperatures significantly below 60°C.
", "Country": "XG", "Code": "Applications", "Name": "Applications" }, { "Language": "en", "Value": "
Vial / BottleCapLabelFunction / DescriptionCatalog NumberContent
1blueLightCycler® EvoScript RNA SYBR® Green I Master,
Master, 5x conc.
Contains enzymes for reverse transcription
and PCR,RT-qPCR Reaction Buffer, dATP,
dCTP, dGTP, and dUTP,
Mg(OAc)2, SYBR® Green I dye,
and proprietary additives.
07 800 134 0012 vials,
440 μl each
07 800 177 00110 vials,
440 μl each
2colorlessLightCycler® EvoScript RNA SYBR® Green I Master,
Water, PCR Grade
To adjust the final reaction volume.07 800 134 0013 vials,
1 ml each
07 800 177 0012 vials,
7.25 ml each
", "Country": "XG", "Code": "Content", "Name": "Content" }, { "Language": "en", "Value": "The LightCycler® EvoScript RNA SYBR® Green I Master consists of 2 different
vials:
  • Vial 1: LightCycler® EvoScript RNA SYBR® Green I Master, 5x conc.
  • Vial 2: Water, PCR Grade
The kit also provides sufficient numbers of vials containing Water, PCR Grade to ensure that fresh (unopened) vials can be used. This minimizes the risk of contamination of RT-qPCR reaction mixes with RNases and other substances.
The resulting RT-qPCR reaction mix also has a hot start Taq DNA Polymerase, nucleotides, and additives ensuring a hot start amplification system with high specificity. The mix contains an optimized concentration of Mg(OAc)2, eliminating the need for additional adjustments. For greater convenience, the RT-qPCR mixture can be used for both RNA and/or DNA templates in parallel.", "Country": "XG", "Code": "Principle", "Name": "Principle" }, { "Language": "en", "Value": "The LightCycler® EvoScript RNA SYBR® Green I Master as a 1-vial composition, is designed for sensitive, high-specificity, high-precision one-step RT-qPCR especially for gene expression assays.", "Country": "XG", "Code": "Product Description", "Name": "Product Description" }, { "Language": "en", "Value": "105 minutes (includes a 15 minute reverse transcription step and 45 cycles of PCR)", "Country": "XG", "Code": "Assay Time", "Name": "Assay Time" } ] } } ] }

LightCycler® EvoScript RNA SYBR® Green I Master

Easy-to-use reaction mix for one-step RT-qPCR using the LightCycler® 480 or the LightCycler® 96 Real-Time PCR Systems

LightCycler<sup><sup>®</sup></sup> EvoScript RNA SYBR<sup><sup>®</sup></sup> Green I Master

Ordering Information

Overview

Technical Documents

Access Material Data Sheets, Certificates of Analysis, and other product documentation.

After clicking above, you will be redirected to eLabDoc, where you can choose your local country.

Instruments

...
    ...
    error errorMessage
    Sorry, we couldn't find the content you are looking for
    Please try again later